Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis
Variations in the concentration of glucoraphanin (GRA) and glucoerucin (GER), as well as the corresponding breakdown products, isothiocyanates (ITCs) and nitriles, were investigated during the growth of broccoli sprouts. The concentrations of GRA and GER decreased sharply from 33.66 µmol/g to 11.48...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/6/750 |
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| author | Jiansheng Wang Yusen Shen Xiaoguang Sheng Huifang Yu Mengfei Song Qiaomei Wang Honghui Gu |
| author_facet | Jiansheng Wang Yusen Shen Xiaoguang Sheng Huifang Yu Mengfei Song Qiaomei Wang Honghui Gu |
| author_sort | Jiansheng Wang |
| collection | DOAJ |
| description | Variations in the concentration of glucoraphanin (GRA) and glucoerucin (GER), as well as the corresponding breakdown products, isothiocyanates (ITCs) and nitriles, were investigated during the growth of broccoli sprouts. The concentrations of GRA and GER decreased sharply from 33.66 µmol/g to 11.48 µmol/g and 12.98 µmol/g to 8.23 µmol/g, respectively, after seed germination. From the third to the seventh day, both GRA and GER were maintained as relatively stable. The highest concentrations of sulforaphane (17.16 µmol/g) and erucin (12.26 µmol/g) were observed on the first day. Hereafter, the concentrations of nitrile hydrolyzed from GRA or GER were higher than those of the corresponding ITCs. Moreover, the ratio of sulforaphane to sulforaphane nitrile decreased from 1.35 to 0.164 from 1 d to 5 d, with a similar trend exhibited for erucin/erucin nitrile after 2 d. RNA-seq analysis showed that <i>BolMYB28</i> and <i>BolCYP83A1</i>, involved in aliphatic glucosinolate (GSL) biosynthesis, remained largely unexpressed until the third day. In contrast, the genes operating within the GSL-myrosinase hydrolysis pathway were highly expressed right from the beginning, with their expression levels increasing significantly after the third day. Additionally, we identified two <i>BolESPs</i> and six <i>BolNSPs</i> that might play important roles in promoting the production of nitriles during the development of broccoli sprouts. |
| first_indexed | 2024-04-24T17:53:58Z |
| format | Article |
| id | doaj.art-91c4744e1774415db3b32ebe733ec054 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T17:53:58Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-91c4744e1774415db3b32ebe733ec0542024-03-27T14:00:32ZengMDPI AGPlants2223-77472024-03-0113675010.3390/plants13060750Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome AnalysisJiansheng Wang0Yusen Shen1Xiaoguang Sheng2Huifang Yu3Mengfei Song4Qiaomei Wang5Honghui Gu6Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Department of Horticulture, Zhejiang University, Hangzhou 310058, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaVariations in the concentration of glucoraphanin (GRA) and glucoerucin (GER), as well as the corresponding breakdown products, isothiocyanates (ITCs) and nitriles, were investigated during the growth of broccoli sprouts. The concentrations of GRA and GER decreased sharply from 33.66 µmol/g to 11.48 µmol/g and 12.98 µmol/g to 8.23 µmol/g, respectively, after seed germination. From the third to the seventh day, both GRA and GER were maintained as relatively stable. The highest concentrations of sulforaphane (17.16 µmol/g) and erucin (12.26 µmol/g) were observed on the first day. Hereafter, the concentrations of nitrile hydrolyzed from GRA or GER were higher than those of the corresponding ITCs. Moreover, the ratio of sulforaphane to sulforaphane nitrile decreased from 1.35 to 0.164 from 1 d to 5 d, with a similar trend exhibited for erucin/erucin nitrile after 2 d. RNA-seq analysis showed that <i>BolMYB28</i> and <i>BolCYP83A1</i>, involved in aliphatic glucosinolate (GSL) biosynthesis, remained largely unexpressed until the third day. In contrast, the genes operating within the GSL-myrosinase hydrolysis pathway were highly expressed right from the beginning, with their expression levels increasing significantly after the third day. Additionally, we identified two <i>BolESPs</i> and six <i>BolNSPs</i> that might play important roles in promoting the production of nitriles during the development of broccoli sprouts.https://www.mdpi.com/2223-7747/13/6/750broccoli sproutglucosinolateRNA-seqsulforaphaneerucin |
| spellingShingle | Jiansheng Wang Yusen Shen Xiaoguang Sheng Huifang Yu Mengfei Song Qiaomei Wang Honghui Gu Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis Plants broccoli sprout glucosinolate RNA-seq sulforaphane erucin |
| title | Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis |
| title_full | Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis |
| title_fullStr | Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis |
| title_full_unstemmed | Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis |
| title_short | Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis |
| title_sort | unravelling glucoraphanin and glucoerucin metabolism across broccoli sprout development insights from metabolite and transcriptome analysis |
| topic | broccoli sprout glucosinolate RNA-seq sulforaphane erucin |
| url | https://www.mdpi.com/2223-7747/13/6/750 |
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