Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
Background: CDIPT (CDP-diacylglycerol–inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-07-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345814000414 |
| _version_ | 1818144935593902080 |
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| author | Changzhen Fu Hong Wang Yaokun Li Jiali Wang Gong Cheng Hongbao Wang Wucai Yang Linsen Zan |
| author_facet | Changzhen Fu Hong Wang Yaokun Li Jiali Wang Gong Cheng Hongbao Wang Wucai Yang Linsen Zan |
| author_sort | Changzhen Fu |
| collection | DOAJ |
| description | Background: CDIPT (CDP-diacylglycerol–inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells.
Results: In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality traits of Qinchuan cattle. Blood samples were obtained from 638 Qinchuan cattle aged from 18 to 24 months. DNA sequencing and PCR-restriction fragment length polymorphism (RFLP) were used to find CDIPT gene single nucleotide polymorphism (SNP). Three SNPs g.244T>C (NCBI: rs42069760), g.1496G>A and g.1514G>A were found in this study. g.244T>C located at 5′untranslated region (5′UTR) of exon 1 showed three genotypes: TT, TC and CC. g.1496G>A and g.1514G>A detected the first time were located in intron 3 and showed the same genotypes: GG, GA and AA.
Conclusions: Analysis results showed that these three SNPs were significantly associated with body measurement traits (BMTs) and meat quality traits (MQTs). We suggested that CDIPT gene may have potential effects on BMTs and MQTs and can be used for marker-assisted selection. |
| first_indexed | 2024-12-11T11:55:28Z |
| format | Article |
| id | doaj.art-91c852c503d84bceb2c260eb7533bdb0 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-11T11:55:28Z |
| publishDate | 2014-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-91c852c503d84bceb2c260eb7533bdb02022-12-22T01:08:13ZengElsevierElectronic Journal of Biotechnology0717-34582014-07-0117416216710.1016/j.ejbt.2014.04.001Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattleChangzhen Fu0Hong Wang1Yaokun Li2Jiali Wang3Gong Cheng4Hongbao Wang5Wucai Yang6Linsen Zan7College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR ChinaNational Beef Cattle Improvement Center, Yangling, Shaanxi 712100, PR ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR ChinaBackground: CDIPT (CDP-diacylglycerol–inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells. Results: In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality traits of Qinchuan cattle. Blood samples were obtained from 638 Qinchuan cattle aged from 18 to 24 months. DNA sequencing and PCR-restriction fragment length polymorphism (RFLP) were used to find CDIPT gene single nucleotide polymorphism (SNP). Three SNPs g.244T>C (NCBI: rs42069760), g.1496G>A and g.1514G>A were found in this study. g.244T>C located at 5′untranslated region (5′UTR) of exon 1 showed three genotypes: TT, TC and CC. g.1496G>A and g.1514G>A detected the first time were located in intron 3 and showed the same genotypes: GG, GA and AA. Conclusions: Analysis results showed that these three SNPs were significantly associated with body measurement traits (BMTs) and meat quality traits (MQTs). We suggested that CDIPT gene may have potential effects on BMTs and MQTs and can be used for marker-assisted selection.http://www.sciencedirect.com/science/article/pii/S0717345814000414Body measurement traitsGene expressionMeat quality traitsPCR-RFLPSNP |
| spellingShingle | Changzhen Fu Hong Wang Yaokun Li Jiali Wang Gong Cheng Hongbao Wang Wucai Yang Linsen Zan Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle Electronic Journal of Biotechnology Body measurement traits Gene expression Meat quality traits PCR-RFLP SNP |
| title | Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle |
| title_full | Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle |
| title_fullStr | Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle |
| title_full_unstemmed | Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle |
| title_short | Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle |
| title_sort | expression profiles and polymorphism analysis of cdipt gene on qinchuan cattle |
| topic | Body measurement traits Gene expression Meat quality traits PCR-RFLP SNP |
| url | http://www.sciencedirect.com/science/article/pii/S0717345814000414 |
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