Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility Study
A systematic study of fabricating nanoparticles (NPs) by cost-effective polymer deposition/solvent displacement (nanoprecipitation) method has been carried out. Five amino acid based biodegradable (AABB) ester polymers (four neutral and one cationic), four organic solvents miscible with water, and e...
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| Format: | Article |
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MDPI AG
2016-12-01
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| Series: | Applied Sciences |
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| Online Access: | http://www.mdpi.com/2076-3417/6/12/444 |
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| author | Temur Kantaria Tengiz Kantaria Sophio Kobauri Mariam Ksovreli Tinatin Kachlishvili Nina Kulikova David Tugushi Ramaz Katsarava |
| author_facet | Temur Kantaria Tengiz Kantaria Sophio Kobauri Mariam Ksovreli Tinatin Kachlishvili Nina Kulikova David Tugushi Ramaz Katsarava |
| author_sort | Temur Kantaria |
| collection | DOAJ |
| description | A systematic study of fabricating nanoparticles (NPs) by cost-effective polymer deposition/solvent displacement (nanoprecipitation) method has been carried out. Five amino acid based biodegradable (AABB) ester polymers (four neutral and one cationic), four organic solvents miscible with water, and eight surfactants were tested for the fabrication of the goal NPs. Depending on the nature of the AABB polymers, organic solvents and surfactants, as well as on the fabrication conditions, the size (Mean Particle Diameter) of the NPs could be tuned within 42 ÷ 398 nm, the zeta-potential within 12.5 ÷ +28 mV. The stability (resuspendability) of the NPs upon storage (at room temperature and refrigerated) was tested as well. In Vitro biocompatibility study of the NPs was performed with four different stable cell lines: A549, HeLa (human); RAW264.7, Hepa 1-6 (murine). Comparing the NPs parameters, their stability upon storage, and the data of biological examinations the best were found: As the AABB polymer, a poly(ester amide) composed of l-leucine, 1,6-hexanediol and sebacic acid–8L6, as a solvent (organic phase—DMSO), and as a surfactant, Tween 20. |
| first_indexed | 2024-12-20T09:35:13Z |
| format | Article |
| id | doaj.art-91ca6ce66c3f44d58cb269375f4f619b |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-20T09:35:13Z |
| publishDate | 2016-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-91ca6ce66c3f44d58cb269375f4f619b2022-12-21T19:45:01ZengMDPI AGApplied Sciences2076-34172016-12-0161244410.3390/app6120444app6120444Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility StudyTemur Kantaria0Tengiz Kantaria1Sophio Kobauri2Mariam Ksovreli3Tinatin Kachlishvili4Nina Kulikova5David Tugushi6Ramaz Katsarava7Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi 0159, GeorgiaInstitute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi 0159, GeorgiaInstitute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi 0159, GeorgiaCellular Immunology Laboratory, Agricultural University of Georgia, Tbilisi 0159, GeorgiaCellular Immunology Laboratory, Agricultural University of Georgia, Tbilisi 0159, GeorgiaCellular Immunology Laboratory, Agricultural University of Georgia, Tbilisi 0159, GeorgiaInstitute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi 0159, GeorgiaInstitute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi 0159, GeorgiaA systematic study of fabricating nanoparticles (NPs) by cost-effective polymer deposition/solvent displacement (nanoprecipitation) method has been carried out. Five amino acid based biodegradable (AABB) ester polymers (four neutral and one cationic), four organic solvents miscible with water, and eight surfactants were tested for the fabrication of the goal NPs. Depending on the nature of the AABB polymers, organic solvents and surfactants, as well as on the fabrication conditions, the size (Mean Particle Diameter) of the NPs could be tuned within 42 ÷ 398 nm, the zeta-potential within 12.5 ÷ +28 mV. The stability (resuspendability) of the NPs upon storage (at room temperature and refrigerated) was tested as well. In Vitro biocompatibility study of the NPs was performed with four different stable cell lines: A549, HeLa (human); RAW264.7, Hepa 1-6 (murine). Comparing the NPs parameters, their stability upon storage, and the data of biological examinations the best were found: As the AABB polymer, a poly(ester amide) composed of l-leucine, 1,6-hexanediol and sebacic acid–8L6, as a solvent (organic phase—DMSO), and as a surfactant, Tween 20.http://www.mdpi.com/2076-3417/6/12/444amino acidsbiodegradable polymersnanoprecipitationnanoparticlessystematic studynanoparticles stabilityin vitro cell compatibility |
| spellingShingle | Temur Kantaria Tengiz Kantaria Sophio Kobauri Mariam Ksovreli Tinatin Kachlishvili Nina Kulikova David Tugushi Ramaz Katsarava Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility Study Applied Sciences amino acids biodegradable polymers nanoprecipitation nanoparticles systematic study nanoparticles stability in vitro cell compatibility |
| title | Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility Study |
| title_full | Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility Study |
| title_fullStr | Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility Study |
| title_full_unstemmed | Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility Study |
| title_short | Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility Study |
| title_sort | biodegradable nanoparticles made of amino acid based ester polymers preparation characterization and in vitro biocompatibility study |
| topic | amino acids biodegradable polymers nanoprecipitation nanoparticles systematic study nanoparticles stability in vitro cell compatibility |
| url | http://www.mdpi.com/2076-3417/6/12/444 |
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