The Effect of Proline on the Freeze-Drying Survival Rate of <i>Bifidobacterium longum</i> CCFM 1029 and Its Inherent Mechanism
Amino acids, which are important compatible solutes, play a significant role in probiotic lyophilization. However, studies on the functions of <i>Bifidobacterium</i> during freeze-drying are limited. Therefore, in this study, we compared the freeze-drying survival rate of <i>Bifido...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13500 |
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| author | Shumao Cui Wenrui Zhou Xin Tang Qiuxiang Zhang Bo Yang Jianxin Zhao Bingyong Mao Hao Zhang |
| author_facet | Shumao Cui Wenrui Zhou Xin Tang Qiuxiang Zhang Bo Yang Jianxin Zhao Bingyong Mao Hao Zhang |
| author_sort | Shumao Cui |
| collection | DOAJ |
| description | Amino acids, which are important compatible solutes, play a significant role in probiotic lyophilization. However, studies on the functions of <i>Bifidobacterium</i> during freeze-drying are limited. Therefore, in this study, we compared the freeze-drying survival rate of <i>Bifidobacterium longum</i> CCFM 1029 cultivated in different media containing different kinds of compatible solutes. We found that the addition of 21 g/L proline to the culture media substantially improved the freeze-drying survival rate of <i>B. longum</i> CCFM 1029 from 18.61 ± 0.42% to 38.74 ± 1.58%. Interestingly, this change has only been observed when the osmotic pressure of the external culture environment is increased. Under these conditions, we found that proline accumulation in this strain increased significantly. This change also helped the strain to maintain its membrane integrity and the activity of some key enzymes during freeze-drying. Overall, these results show that the addition of proline can help the strain resist a tough environment during lyophilization. The findings of this study provide preliminary data for producers of probiotics who wish to achieve higher freeze-drying survival rates during industrial production. |
| first_indexed | 2024-03-09T18:59:45Z |
| format | Article |
| id | doaj.art-91cb88f82f954257b9b9db671a82eb1a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:59:45Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-91cb88f82f954257b9b9db671a82eb1a2023-11-24T05:08:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211350010.3390/ijms232113500The Effect of Proline on the Freeze-Drying Survival Rate of <i>Bifidobacterium longum</i> CCFM 1029 and Its Inherent MechanismShumao Cui0Wenrui Zhou1Xin Tang2Qiuxiang Zhang3Bo Yang4Jianxin Zhao5Bingyong Mao6Hao Zhang7State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaAmino acids, which are important compatible solutes, play a significant role in probiotic lyophilization. However, studies on the functions of <i>Bifidobacterium</i> during freeze-drying are limited. Therefore, in this study, we compared the freeze-drying survival rate of <i>Bifidobacterium longum</i> CCFM 1029 cultivated in different media containing different kinds of compatible solutes. We found that the addition of 21 g/L proline to the culture media substantially improved the freeze-drying survival rate of <i>B. longum</i> CCFM 1029 from 18.61 ± 0.42% to 38.74 ± 1.58%. Interestingly, this change has only been observed when the osmotic pressure of the external culture environment is increased. Under these conditions, we found that proline accumulation in this strain increased significantly. This change also helped the strain to maintain its membrane integrity and the activity of some key enzymes during freeze-drying. Overall, these results show that the addition of proline can help the strain resist a tough environment during lyophilization. The findings of this study provide preliminary data for producers of probiotics who wish to achieve higher freeze-drying survival rates during industrial production.https://www.mdpi.com/1422-0067/23/21/13500proline<i>Bifidobacterium longum</i> CCFM 1029freeze-drying survival ratemechanism |
| spellingShingle | Shumao Cui Wenrui Zhou Xin Tang Qiuxiang Zhang Bo Yang Jianxin Zhao Bingyong Mao Hao Zhang The Effect of Proline on the Freeze-Drying Survival Rate of <i>Bifidobacterium longum</i> CCFM 1029 and Its Inherent Mechanism International Journal of Molecular Sciences proline <i>Bifidobacterium longum</i> CCFM 1029 freeze-drying survival rate mechanism |
| title | The Effect of Proline on the Freeze-Drying Survival Rate of <i>Bifidobacterium longum</i> CCFM 1029 and Its Inherent Mechanism |
| title_full | The Effect of Proline on the Freeze-Drying Survival Rate of <i>Bifidobacterium longum</i> CCFM 1029 and Its Inherent Mechanism |
| title_fullStr | The Effect of Proline on the Freeze-Drying Survival Rate of <i>Bifidobacterium longum</i> CCFM 1029 and Its Inherent Mechanism |
| title_full_unstemmed | The Effect of Proline on the Freeze-Drying Survival Rate of <i>Bifidobacterium longum</i> CCFM 1029 and Its Inherent Mechanism |
| title_short | The Effect of Proline on the Freeze-Drying Survival Rate of <i>Bifidobacterium longum</i> CCFM 1029 and Its Inherent Mechanism |
| title_sort | effect of proline on the freeze drying survival rate of i bifidobacterium longum i ccfm 1029 and its inherent mechanism |
| topic | proline <i>Bifidobacterium longum</i> CCFM 1029 freeze-drying survival rate mechanism |
| url | https://www.mdpi.com/1422-0067/23/21/13500 |
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