Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers
This study investigated the genetic diversity and population structure of 63 genotypes from Turkish bread wheat germplasm using iPBS-retrotransposons primers. The thirty-four iPBS primers produced a total of 1231 polymorphic bands, ranging from 8 (iPBS-2375) to 60 (iPBS-2381) alleles per marker, wit...
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MDPI AG
2023-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/1/255 |
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| author | Kamil Haliloğlu Aras Türkoğlu Ali Öztürk Gniewko Niedbała Mohsen Niazian Tomasz Wojciechowski Magdalena Piekutowska |
| author_facet | Kamil Haliloğlu Aras Türkoğlu Ali Öztürk Gniewko Niedbała Mohsen Niazian Tomasz Wojciechowski Magdalena Piekutowska |
| author_sort | Kamil Haliloğlu |
| collection | DOAJ |
| description | This study investigated the genetic diversity and population structure of 63 genotypes from Turkish bread wheat germplasm using iPBS-retrotransposons primers. The thirty-four iPBS primers produced a total of 1231 polymorphic bands, ranging from 8 (iPBS-2375) to 60 (iPBS-2381) alleles per marker, with an average number of 36.00 alleles. The polymorphism information content (PIC) per marker varied between 0.048 (iPBS 2087) and 0.303 (iPBS 2382), with an average of 0.175. The numbers of effective alleles (ne), genetic diversity of Nei (h), and Shannon’s information index (I) value were calculated as 1.157, 0.95, and 0.144, respectively. The greatest genetic distance (0.164) was between Eastern Anatolia Agricultural Research Institute genotypes and Çukurova Agricultural Research Institute genotypes. The unweighted pair-group method with arithmetic mean (UPGMA) dendrogram placed the 63 wheat genotypes into three clusters. The percentage of genetic diversity explained by each of the three main coordinates of the basic coordinate analysis was determined to be 44.58, 12.08, and 3.44, respectively. AMOVA (Analysis of Molecular Variance) showed that the variation within populations was 99% and that between populations was 1%. The result of genetic structure analysis suggests that the greatest value of K was calculated as 3. The F-statistic (Fst) value was determined as 0.4005, 0.2374, and 0.3773 in the first to third subpopulations, respectively. Likewise, the expected heterozygosity values (He) were determined as 0.2203, 0.2599, and 0.2155 in the first, second, and third subpopulations, respectively. According to the information obtained in the study, the most genetically distant genotypes were the G1 (Aksel 2000) and G63 (Karasu 90) genotypes. This study provided a deep insight into genetic variations in Turkish bread wheat germplasm using the iPBS-retrotransposons marker system. |
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| id | doaj.art-91cffdd72c0044a993c99bcfe1364305 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:50:14Z |
| publishDate | 2023-01-01 |
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| series | Agronomy |
| spelling | doaj.art-91cffdd72c0044a993c99bcfe13643052023-11-30T20:52:04ZengMDPI AGAgronomy2073-43952023-01-0113125510.3390/agronomy13010255Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based MarkersKamil Haliloğlu0Aras Türkoğlu1Ali Öztürk2Gniewko Niedbała3Mohsen Niazian4Tomasz Wojciechowski5Magdalena Piekutowska6Department of Field Crops, Faculty of Agriculture, Ataturk University, 25240 Erzurum, TurkeyDepartment of Field Crops, Faculty of Agriculture, Necmettin Erbakan University, 42310 Konya, TurkeyDepartment of Field Crops, Faculty of Agriculture, Ataturk University, 25240 Erzurum, TurkeyDepartment of Biosystems Engineering, Faculty of Environmental and Mechanical Engineering, Poznań University of Life Sciences, Wojska Polskiego 50, 60-627 Poznań, PolandField and Horticultural Crops Research Department, Kurdistan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sanandaj 6616936311, IranDepartment of Biosystems Engineering, Faculty of Environmental and Mechanical Engineering, Poznań University of Life Sciences, Wojska Polskiego 50, 60-627 Poznań, PolandDepartment of Geoecology and Geoinformation, Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Partyzantów 27, 76-200 Słupsk, PolandThis study investigated the genetic diversity and population structure of 63 genotypes from Turkish bread wheat germplasm using iPBS-retrotransposons primers. The thirty-four iPBS primers produced a total of 1231 polymorphic bands, ranging from 8 (iPBS-2375) to 60 (iPBS-2381) alleles per marker, with an average number of 36.00 alleles. The polymorphism information content (PIC) per marker varied between 0.048 (iPBS 2087) and 0.303 (iPBS 2382), with an average of 0.175. The numbers of effective alleles (ne), genetic diversity of Nei (h), and Shannon’s information index (I) value were calculated as 1.157, 0.95, and 0.144, respectively. The greatest genetic distance (0.164) was between Eastern Anatolia Agricultural Research Institute genotypes and Çukurova Agricultural Research Institute genotypes. The unweighted pair-group method with arithmetic mean (UPGMA) dendrogram placed the 63 wheat genotypes into three clusters. The percentage of genetic diversity explained by each of the three main coordinates of the basic coordinate analysis was determined to be 44.58, 12.08, and 3.44, respectively. AMOVA (Analysis of Molecular Variance) showed that the variation within populations was 99% and that between populations was 1%. The result of genetic structure analysis suggests that the greatest value of K was calculated as 3. The F-statistic (Fst) value was determined as 0.4005, 0.2374, and 0.3773 in the first to third subpopulations, respectively. Likewise, the expected heterozygosity values (He) were determined as 0.2203, 0.2599, and 0.2155 in the first, second, and third subpopulations, respectively. According to the information obtained in the study, the most genetically distant genotypes were the G1 (Aksel 2000) and G63 (Karasu 90) genotypes. This study provided a deep insight into genetic variations in Turkish bread wheat germplasm using the iPBS-retrotransposons marker system.https://www.mdpi.com/2073-4395/13/1/255wheatgermplasmgenetic diversityenvironmental conditionsgenotypesinter-primer binding site |
| spellingShingle | Kamil Haliloğlu Aras Türkoğlu Ali Öztürk Gniewko Niedbała Mohsen Niazian Tomasz Wojciechowski Magdalena Piekutowska Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers Agronomy wheat germplasm genetic diversity environmental conditions genotypes inter-primer binding site |
| title | Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers |
| title_full | Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers |
| title_fullStr | Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers |
| title_full_unstemmed | Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers |
| title_short | Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers |
| title_sort | genetic diversity and population structure in bread wheat germplasm from turkiye using ipbs retrotransposons based markers |
| topic | wheat germplasm genetic diversity environmental conditions genotypes inter-primer binding site |
| url | https://www.mdpi.com/2073-4395/13/1/255 |
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