Biocontrol of <i>Phytophthora xcambivora</i> on <i>Castanea sativa</i>: Selection of Local <i>Trichoderma</i> spp. Isolates for the Management of Ink Disease
Ink disease is a devastating disease of chestnut (<i>Castanea sativa</i>) worldwide, caused by <i>Phytophthora</i> species. The only management measures of this disease are chemical and agronomic interventions. This work focuses on the evaluation of the in vitro antagonistic...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/1999-4907/13/7/1065 |
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| author | Arcangela Frascella Sabrina Sarrocco Antonietta Mello Francesco Venice Cristina Salvatici Roberto Danti Giovanni Emiliani Sara Barberini Gianni Della Rocca |
| author_facet | Arcangela Frascella Sabrina Sarrocco Antonietta Mello Francesco Venice Cristina Salvatici Roberto Danti Giovanni Emiliani Sara Barberini Gianni Della Rocca |
| author_sort | Arcangela Frascella |
| collection | DOAJ |
| description | Ink disease is a devastating disease of chestnut (<i>Castanea sativa</i>) worldwide, caused by <i>Phytophthora</i> species. The only management measures of this disease are chemical and agronomic interventions. This work focuses on the evaluation of the in vitro antagonistic capacity of 20 isolates of <i>Trichoderma</i> spp. selected in a diseased chestnut orchard in Tuscan Apennines (San Godenzo, Italy) for the biocontrol of <i>Phytophthora xcambivora</i>. Each <i>Trichoderma</i> isolate was tested to investigate pathogen inhibition capability by antagonism in dual cultures and antibiosis by secondary metabolites production (diffusible and Volatile Organic Compounds). The six most performing isolates of <i>Trichoderma</i> spp. were further assessed for their aptitude to synthesize chitinase, glucanase and cellulase, and to act as mycoparasite. All six selected isolates displayed the capability to control the pathogen in vitro by synergistically coupling antibiosis and mycoparasitism at different levels regardless of the species they belong to, but rather, in relation to specific features of the single genotypes. In particular, <i>T. hamatum</i> SG18 and <i>T. koningiopsis</i> SG6 displayed the most promising results in pathogen inhibition, thus further investigations are needed to confirm their in vivo efficacy. |
| first_indexed | 2024-03-09T10:19:24Z |
| format | Article |
| id | doaj.art-91d056415c1145a0ba40945b18d8b853 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-09T10:19:24Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-91d056415c1145a0ba40945b18d8b8532023-12-01T22:09:36ZengMDPI AGForests1999-49072022-07-01137106510.3390/f13071065Biocontrol of <i>Phytophthora xcambivora</i> on <i>Castanea sativa</i>: Selection of Local <i>Trichoderma</i> spp. Isolates for the Management of Ink DiseaseArcangela Frascella0Sabrina Sarrocco1Antonietta Mello2Francesco Venice3Cristina Salvatici4Roberto Danti5Giovanni Emiliani6Sara Barberini7Gianni Della Rocca8Institute for Sustainable Plant Protection (IPSP), SS Sesto Fiorentino, National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 50124 Pisa, ItalyInstitute for Sustainable Plant Protection (IPSP), SS Turin, National Research Council (CNR), Viale Mattioli 25, 10125 Turin, ItalyInstitute for Sustainable Plant Protection (IPSP), SS Turin, National Research Council (CNR), Viale Mattioli 25, 10125 Turin, ItalyInstitute of Chemistry of Organometallic Compounds (ICCOM), Electron Microscopy Centre (Ce.M.E.), National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyInstitute for Sustainable Plant Protection (IPSP), SS Sesto Fiorentino, National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyInstitute for Sustainable Plant Protection (IPSP), SS Sesto Fiorentino, National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyInstitute for Sustainable Plant Protection (IPSP), SS Sesto Fiorentino, National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyInstitute for Sustainable Plant Protection (IPSP), SS Sesto Fiorentino, National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyInk disease is a devastating disease of chestnut (<i>Castanea sativa</i>) worldwide, caused by <i>Phytophthora</i> species. The only management measures of this disease are chemical and agronomic interventions. This work focuses on the evaluation of the in vitro antagonistic capacity of 20 isolates of <i>Trichoderma</i> spp. selected in a diseased chestnut orchard in Tuscan Apennines (San Godenzo, Italy) for the biocontrol of <i>Phytophthora xcambivora</i>. Each <i>Trichoderma</i> isolate was tested to investigate pathogen inhibition capability by antagonism in dual cultures and antibiosis by secondary metabolites production (diffusible and Volatile Organic Compounds). The six most performing isolates of <i>Trichoderma</i> spp. were further assessed for their aptitude to synthesize chitinase, glucanase and cellulase, and to act as mycoparasite. All six selected isolates displayed the capability to control the pathogen in vitro by synergistically coupling antibiosis and mycoparasitism at different levels regardless of the species they belong to, but rather, in relation to specific features of the single genotypes. In particular, <i>T. hamatum</i> SG18 and <i>T. koningiopsis</i> SG6 displayed the most promising results in pathogen inhibition, thus further investigations are needed to confirm their in vivo efficacy.https://www.mdpi.com/1999-4907/13/7/1065antagonismantibiosissecondary metabolitesSEMenzymatic activity |
| spellingShingle | Arcangela Frascella Sabrina Sarrocco Antonietta Mello Francesco Venice Cristina Salvatici Roberto Danti Giovanni Emiliani Sara Barberini Gianni Della Rocca Biocontrol of <i>Phytophthora xcambivora</i> on <i>Castanea sativa</i>: Selection of Local <i>Trichoderma</i> spp. Isolates for the Management of Ink Disease Forests antagonism antibiosis secondary metabolites SEM enzymatic activity |
| title | Biocontrol of <i>Phytophthora xcambivora</i> on <i>Castanea sativa</i>: Selection of Local <i>Trichoderma</i> spp. Isolates for the Management of Ink Disease |
| title_full | Biocontrol of <i>Phytophthora xcambivora</i> on <i>Castanea sativa</i>: Selection of Local <i>Trichoderma</i> spp. Isolates for the Management of Ink Disease |
| title_fullStr | Biocontrol of <i>Phytophthora xcambivora</i> on <i>Castanea sativa</i>: Selection of Local <i>Trichoderma</i> spp. Isolates for the Management of Ink Disease |
| title_full_unstemmed | Biocontrol of <i>Phytophthora xcambivora</i> on <i>Castanea sativa</i>: Selection of Local <i>Trichoderma</i> spp. Isolates for the Management of Ink Disease |
| title_short | Biocontrol of <i>Phytophthora xcambivora</i> on <i>Castanea sativa</i>: Selection of Local <i>Trichoderma</i> spp. Isolates for the Management of Ink Disease |
| title_sort | biocontrol of i phytophthora xcambivora i on i castanea sativa i selection of local i trichoderma i spp isolates for the management of ink disease |
| topic | antagonism antibiosis secondary metabolites SEM enzymatic activity |
| url | https://www.mdpi.com/1999-4907/13/7/1065 |
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