Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into <i>E. coli</i> O157:H7 on <i>Arabidopsis thaliana</i> Rosettes
Many antibiotic resistance genes present in human pathogenic bacteria are believed to originate from environmental bacteria. Conjugation of antibiotic resistance conferring plasmids is considered to be one of the major reasons for the increasing prevalence of antibiotic resistances. A hotspot for pl...
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MDPI AG
2021-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/8/928 |
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| author | Mitja N. P. Remus-Emsermann David Aicher Cosima Pelludat Pascal Gisler David Drissner |
| author_facet | Mitja N. P. Remus-Emsermann David Aicher Cosima Pelludat Pascal Gisler David Drissner |
| author_sort | Mitja N. P. Remus-Emsermann |
| collection | DOAJ |
| description | Many antibiotic resistance genes present in human pathogenic bacteria are believed to originate from environmental bacteria. Conjugation of antibiotic resistance conferring plasmids is considered to be one of the major reasons for the increasing prevalence of antibiotic resistances. A hotspot for plasmid-based horizontal gene transfer is the phyllosphere, i.e., the surfaces of aboveground plant parts. Bacteria in the phyllosphere might serve as intermediate hosts with transfer capability to human pathogenic bacteria. In this study, the exchange of mobilisable and self-transmissible plasmids via conjugation was evaluated. The conjugation from the laboratory strain <i>Escherichia coli</i> S17-1, the model phyllosphere coloniser <i>Pantoea eucalypti</i> 299R, and the model pathogen <i>E. coli</i> O157:H7 to the recipient strain <i>E. coli</i> O157:H7::MRE103 (<i>Ec</i>O157:H7red) in the phyllosphere of <i>Arabidopsis thaliana</i> was determined. The results suggest that short-term occurrence of a competent donor is sufficient to fix plasmids in a recipient population of <i>E. coli</i> O157:H7red. The spread of self-transmissible plasmids was limited after initial steep increases of transconjugants that contributed up to 10% of the total recipient population. The here-presented data of plasmid transfer will be important for future modelling approaches to estimate environmental spread of antibiotic resistance in agricultural production environments. |
| first_indexed | 2024-03-10T09:04:28Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:04:28Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-91d2237536064943bb6d10f09062e5d42023-11-22T06:33:23ZengMDPI AGAntibiotics2079-63822021-07-0110892810.3390/antibiotics10080928Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into <i>E. coli</i> O157:H7 on <i>Arabidopsis thaliana</i> RosettesMitja N. P. Remus-Emsermann0David Aicher1Cosima Pelludat2Pascal Gisler3David Drissner4Microbiology of Plant Foods, Agroscope, 8820 Waedenswil, SwitzerlandDepartment of Life Sciences, Albstadt-Sigmaringen University, 72488 Sigmaringen, GermanyPlant Pathology and Zoology in Fruit and Vegetable Production, Agroscope, 8820 Waedenswil, SwitzerlandMicrobiology of Plant Foods, Agroscope, 8820 Waedenswil, SwitzerlandMicrobiology of Plant Foods, Agroscope, 8820 Waedenswil, SwitzerlandMany antibiotic resistance genes present in human pathogenic bacteria are believed to originate from environmental bacteria. Conjugation of antibiotic resistance conferring plasmids is considered to be one of the major reasons for the increasing prevalence of antibiotic resistances. A hotspot for plasmid-based horizontal gene transfer is the phyllosphere, i.e., the surfaces of aboveground plant parts. Bacteria in the phyllosphere might serve as intermediate hosts with transfer capability to human pathogenic bacteria. In this study, the exchange of mobilisable and self-transmissible plasmids via conjugation was evaluated. The conjugation from the laboratory strain <i>Escherichia coli</i> S17-1, the model phyllosphere coloniser <i>Pantoea eucalypti</i> 299R, and the model pathogen <i>E. coli</i> O157:H7 to the recipient strain <i>E. coli</i> O157:H7::MRE103 (<i>Ec</i>O157:H7red) in the phyllosphere of <i>Arabidopsis thaliana</i> was determined. The results suggest that short-term occurrence of a competent donor is sufficient to fix plasmids in a recipient population of <i>E. coli</i> O157:H7red. The spread of self-transmissible plasmids was limited after initial steep increases of transconjugants that contributed up to 10% of the total recipient population. The here-presented data of plasmid transfer will be important for future modelling approaches to estimate environmental spread of antibiotic resistance in agricultural production environments.https://www.mdpi.com/2079-6382/10/8/928antibiotic resistanceconjugationplasmidsphyllosphere |
| spellingShingle | Mitja N. P. Remus-Emsermann David Aicher Cosima Pelludat Pascal Gisler David Drissner Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into <i>E. coli</i> O157:H7 on <i>Arabidopsis thaliana</i> Rosettes Antibiotics antibiotic resistance conjugation plasmids phyllosphere |
| title | Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into <i>E. coli</i> O157:H7 on <i>Arabidopsis thaliana</i> Rosettes |
| title_full | Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into <i>E. coli</i> O157:H7 on <i>Arabidopsis thaliana</i> Rosettes |
| title_fullStr | Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into <i>E. coli</i> O157:H7 on <i>Arabidopsis thaliana</i> Rosettes |
| title_full_unstemmed | Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into <i>E. coli</i> O157:H7 on <i>Arabidopsis thaliana</i> Rosettes |
| title_short | Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into <i>E. coli</i> O157:H7 on <i>Arabidopsis thaliana</i> Rosettes |
| title_sort | conjugation dynamics of self transmissible and mobilisable plasmids into i e coli i o157 h7 on i arabidopsis thaliana i rosettes |
| topic | antibiotic resistance conjugation plasmids phyllosphere |
| url | https://www.mdpi.com/2079-6382/10/8/928 |
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