Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer.
AIM: Several multigene expression-based tests offering prognostic and predictive information in hormone-receptor positive early breast cancer were established during the last years. These tests provide prognostic information on distant recurrences and can serve as an aid in therapy decisions. We ana...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3591350?pdf=render |
| _version_ | 1811263774132273152 |
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| author | Zsuzsanna Varga Peter Sinn Florian Fritzsche Arthur von Hochstetter Aurelia Noske Peter Schraml Christoph Tausch Andreas Trojan Holger Moch |
| author_facet | Zsuzsanna Varga Peter Sinn Florian Fritzsche Arthur von Hochstetter Aurelia Noske Peter Schraml Christoph Tausch Andreas Trojan Holger Moch |
| author_sort | Zsuzsanna Varga |
| collection | DOAJ |
| description | AIM: Several multigene expression-based tests offering prognostic and predictive information in hormone-receptor positive early breast cancer were established during the last years. These tests provide prognostic information on distant recurrences and can serve as an aid in therapy decisions. We analyzed the recently validated reverse-transcription-quantitative-real-time PCR-based multigene-expression Endopredict (EP)-test on 34 hormone-receptor positive breast-cancer cases and compared the EP scores with the Oncotype DX Recurrence-scores (RS) obtained from the same cancer samples. METHODS: Formalin-fixed, paraffin-embedded invasive breast-cancer tissues from 34 patients were analyzed by the EP-test. Representative tumor blocks were analyzed with Oncotype DX prior to this study. Tumor tissue was removed from unstained slides, total-RNA was isolated and EP-analysis was performed blinded to Oncotype DX results. RESULTS: Extraction of sufficient amounts of RNA and generation of valid EP-scores were possible for all 34 samples. EP classified 11 patients as low-risk and 23 patients as high-risk. RS Score defined 15 patients as low-risk, 10 patients as intermediate-risk in and 9 patients as high-risk. Major-discrepancy occurred in 6 of 34 cases (18%): Low-risk RS was classified as high-risk by EP in 6 cases. Combining the RS intermediate-risk and high-risk groups to a common group, the concordance between both tests was 76%. Correlation between continuous EP and RS-scores was moderate (Pearson-coefficient: 0.65 (p<0.01). CONCLUSION: We observed a significant but moderate concordance (76%) and moderate correlation (0.65) between RS and EP Score. Differences in results can be explained by different weighting of biological motives covered by the two tests. Further studies are needed to explore the clinical relevance of discrepant test results with respect of outcome. |
| first_indexed | 2024-04-12T19:51:17Z |
| format | Article |
| id | doaj.art-91d4e9ded0ea4414a3c023ecbd17f1c5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T19:51:17Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-91d4e9ded0ea4414a3c023ecbd17f1c52022-12-22T03:18:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5848310.1371/journal.pone.0058483Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer.Zsuzsanna VargaPeter SinnFlorian FritzscheArthur von HochstetterAurelia NoskePeter SchramlChristoph TauschAndreas TrojanHolger MochAIM: Several multigene expression-based tests offering prognostic and predictive information in hormone-receptor positive early breast cancer were established during the last years. These tests provide prognostic information on distant recurrences and can serve as an aid in therapy decisions. We analyzed the recently validated reverse-transcription-quantitative-real-time PCR-based multigene-expression Endopredict (EP)-test on 34 hormone-receptor positive breast-cancer cases and compared the EP scores with the Oncotype DX Recurrence-scores (RS) obtained from the same cancer samples. METHODS: Formalin-fixed, paraffin-embedded invasive breast-cancer tissues from 34 patients were analyzed by the EP-test. Representative tumor blocks were analyzed with Oncotype DX prior to this study. Tumor tissue was removed from unstained slides, total-RNA was isolated and EP-analysis was performed blinded to Oncotype DX results. RESULTS: Extraction of sufficient amounts of RNA and generation of valid EP-scores were possible for all 34 samples. EP classified 11 patients as low-risk and 23 patients as high-risk. RS Score defined 15 patients as low-risk, 10 patients as intermediate-risk in and 9 patients as high-risk. Major-discrepancy occurred in 6 of 34 cases (18%): Low-risk RS was classified as high-risk by EP in 6 cases. Combining the RS intermediate-risk and high-risk groups to a common group, the concordance between both tests was 76%. Correlation between continuous EP and RS-scores was moderate (Pearson-coefficient: 0.65 (p<0.01). CONCLUSION: We observed a significant but moderate concordance (76%) and moderate correlation (0.65) between RS and EP Score. Differences in results can be explained by different weighting of biological motives covered by the two tests. Further studies are needed to explore the clinical relevance of discrepant test results with respect of outcome.http://europepmc.org/articles/PMC3591350?pdf=render |
| spellingShingle | Zsuzsanna Varga Peter Sinn Florian Fritzsche Arthur von Hochstetter Aurelia Noske Peter Schraml Christoph Tausch Andreas Trojan Holger Moch Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer. PLoS ONE |
| title | Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer. |
| title_full | Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer. |
| title_fullStr | Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer. |
| title_full_unstemmed | Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer. |
| title_short | Comparison of EndoPredict and Oncotype DX test results in hormone receptor positive invasive breast cancer. |
| title_sort | comparison of endopredict and oncotype dx test results in hormone receptor positive invasive breast cancer |
| url | http://europepmc.org/articles/PMC3591350?pdf=render |
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