Toxocara canis-induced changes in host intestinal microbial communities
Abstract Background Toxocara canis is a roundworm that resides in the gastrointestinal tract of dogs and causes various pathological changes. The dog’s intestinal system consists of a diverse and dynamic bacterial community that has extensive effects on intestinal physiology, immunity and metabolics...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-023-06072-w |
| _version_ | 1797377143754194944 |
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| author | Soben Sieng Ping Chen Na Wang Jing-Yun Xu Qian Han |
| author_facet | Soben Sieng Ping Chen Na Wang Jing-Yun Xu Qian Han |
| author_sort | Soben Sieng |
| collection | DOAJ |
| description | Abstract Background Toxocara canis is a roundworm that resides in the gastrointestinal tract of dogs and causes various pathological changes. The dog’s intestinal system consists of a diverse and dynamic bacterial community that has extensive effects on intestinal physiology, immunity and metabolics. In the case of intestinal parasites, interactions with the host intestinal flora are inevitable during the process of parasitism. Methods We studied the role of T. canis in regulating the composition and diversity of the intestinal flora of the host by high-throughput sequencing of the 16S ribosomal RNA gene and various bioinformatics analyses. Results The α-diversity analysis showed that Toxocara canis infection resulted in a significant decrease in the abundance and diversity of host intestinal flora. The β-diversity analysis showed that the intestinal flora of infected dogs was similar to that carried by T. canis. Analysis of the microflora composition and differences at the phylum level showed that the ratio of Firmicutes to Bacteroidetes (F/B ratio) increased with T. canis infection. Analysis of species composition and differences at the genus level revealed that the proportion of some of the pathogenic bacteria, such as Clostridium sensu stricto and Staphylococcus, increased after T. canis infection. Conclusions Toxocara canis infection affected the composition and diversity of the flora in the host intestinal tract. These results not only shed light on the potential mechanism of T. canis invasion and long-term survival in the intestinal tract, but also provide a new basis for the development of anthelmintic drugs. Graphical Abstract |
| first_indexed | 2024-03-08T19:49:29Z |
| format | Article |
| id | doaj.art-91d9967034044854aed094bc8d968b3f |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-08T19:49:29Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-91d9967034044854aed094bc8d968b3f2023-12-24T12:10:58ZengBMCParasites & Vectors1756-33052023-12-0116111010.1186/s13071-023-06072-wToxocara canis-induced changes in host intestinal microbial communitiesSoben Sieng0Ping Chen1Na Wang2Jing-Yun Xu3Qian Han4Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Health Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Health Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Health Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Health Sciences, Hainan UniversityLaboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Health Sciences, Hainan UniversityAbstract Background Toxocara canis is a roundworm that resides in the gastrointestinal tract of dogs and causes various pathological changes. The dog’s intestinal system consists of a diverse and dynamic bacterial community that has extensive effects on intestinal physiology, immunity and metabolics. In the case of intestinal parasites, interactions with the host intestinal flora are inevitable during the process of parasitism. Methods We studied the role of T. canis in regulating the composition and diversity of the intestinal flora of the host by high-throughput sequencing of the 16S ribosomal RNA gene and various bioinformatics analyses. Results The α-diversity analysis showed that Toxocara canis infection resulted in a significant decrease in the abundance and diversity of host intestinal flora. The β-diversity analysis showed that the intestinal flora of infected dogs was similar to that carried by T. canis. Analysis of the microflora composition and differences at the phylum level showed that the ratio of Firmicutes to Bacteroidetes (F/B ratio) increased with T. canis infection. Analysis of species composition and differences at the genus level revealed that the proportion of some of the pathogenic bacteria, such as Clostridium sensu stricto and Staphylococcus, increased after T. canis infection. Conclusions Toxocara canis infection affected the composition and diversity of the flora in the host intestinal tract. These results not only shed light on the potential mechanism of T. canis invasion and long-term survival in the intestinal tract, but also provide a new basis for the development of anthelmintic drugs. Graphical Abstracthttps://doi.org/10.1186/s13071-023-06072-wToxocara canisIntestinal flora16S rRNA high-throughput sequencing |
| spellingShingle | Soben Sieng Ping Chen Na Wang Jing-Yun Xu Qian Han Toxocara canis-induced changes in host intestinal microbial communities Parasites & Vectors Toxocara canis Intestinal flora 16S rRNA high-throughput sequencing |
| title | Toxocara canis-induced changes in host intestinal microbial communities |
| title_full | Toxocara canis-induced changes in host intestinal microbial communities |
| title_fullStr | Toxocara canis-induced changes in host intestinal microbial communities |
| title_full_unstemmed | Toxocara canis-induced changes in host intestinal microbial communities |
| title_short | Toxocara canis-induced changes in host intestinal microbial communities |
| title_sort | toxocara canis induced changes in host intestinal microbial communities |
| topic | Toxocara canis Intestinal flora 16S rRNA high-throughput sequencing |
| url | https://doi.org/10.1186/s13071-023-06072-w |
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