Lab-Scale Optimization of <em>Aurantiochytrium</em> sp. Culture Medium for Improved Growth and DHA Production
Thraustochytrids have gained increasing relevance over the last decades, due to their fast growth and outstanding capacity to accumulate polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). In this context, the present work aimed to optimize the growth performance and DHA yi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/7/2500 |
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| author | Mafalda Trovão Hugo Pereira Margarida Costa Adriana Machado Ana Barros Maria Soares Bernardo Carvalho Joana Teles Silva João Varela Joana Silva |
| author_facet | Mafalda Trovão Hugo Pereira Margarida Costa Adriana Machado Ana Barros Maria Soares Bernardo Carvalho Joana Teles Silva João Varela Joana Silva |
| author_sort | Mafalda Trovão |
| collection | DOAJ |
| description | Thraustochytrids have gained increasing relevance over the last decades, due to their fast growth and outstanding capacity to accumulate polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). In this context, the present work aimed to optimize the growth performance and DHA yields by improving the culture medium of <i>Aurantiochytrium</i> sp. AF0043. Accordingly, two distinct culture media were optimized: (i) an inorganic optimized medium (IOM), containing only monosodium glutamate and glucose as nitrogen and carbon sources, respectively; and (ii) an organic and sustainable waste-based optimized medium (WOM), containing corn steep powder and glycerol, added in fed-batch mode, as nitrogen and carbon sources, respectively. Overall, the lab-scale optimization allowed to increase the biomass yield 1.5-fold and enhance DHA content 1.7-fold using IOM. Moreover, WOM enabled a 2-fold increase in biomass yield and a significant improvement in lipid contents, from 22.78% to 31.14%. However, DHA content was enhanced almost 3-fold, from an initial content of 10.12% to 29.66% of total fatty acids contained in the biomass. Therefore, these results strongly suggest, not only that the production pipeline was significantly improved but also confirmed the potential use of <i>Aurantiochytrium</i> sp. AF0043 as a source of DHA. |
| first_indexed | 2024-03-10T20:40:13Z |
| format | Article |
| id | doaj.art-91d9f1df082e4934a14ed3f7f15d3550 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T20:40:13Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-91d9f1df082e4934a14ed3f7f15d35502023-11-19T20:46:13ZengMDPI AGApplied Sciences2076-34172020-04-01107250010.3390/app10072500Lab-Scale Optimization of <em>Aurantiochytrium</em> sp. Culture Medium for Improved Growth and DHA ProductionMafalda Trovão0Hugo Pereira1Margarida Costa2Adriana Machado3Ana Barros4Maria Soares5Bernardo Carvalho6Joana Teles Silva7João Varela8Joana Silva9Allmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, PortugalCCMAR—Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, PortugalAllmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, PortugalAllmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, PortugalAllmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, PortugalAllmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, PortugalAllmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, PortugalAllmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, PortugalCCMAR—Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, PortugalAllmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, PortugalThraustochytrids have gained increasing relevance over the last decades, due to their fast growth and outstanding capacity to accumulate polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). In this context, the present work aimed to optimize the growth performance and DHA yields by improving the culture medium of <i>Aurantiochytrium</i> sp. AF0043. Accordingly, two distinct culture media were optimized: (i) an inorganic optimized medium (IOM), containing only monosodium glutamate and glucose as nitrogen and carbon sources, respectively; and (ii) an organic and sustainable waste-based optimized medium (WOM), containing corn steep powder and glycerol, added in fed-batch mode, as nitrogen and carbon sources, respectively. Overall, the lab-scale optimization allowed to increase the biomass yield 1.5-fold and enhance DHA content 1.7-fold using IOM. Moreover, WOM enabled a 2-fold increase in biomass yield and a significant improvement in lipid contents, from 22.78% to 31.14%. However, DHA content was enhanced almost 3-fold, from an initial content of 10.12% to 29.66% of total fatty acids contained in the biomass. Therefore, these results strongly suggest, not only that the production pipeline was significantly improved but also confirmed the potential use of <i>Aurantiochytrium</i> sp. AF0043 as a source of DHA.https://www.mdpi.com/2076-3417/10/7/2500thraustochytrids<i>Aurantiochytrium</i> sp.docosahexaenoic acid (DHA)culture medium optimizationgrowth performance |
| spellingShingle | Mafalda Trovão Hugo Pereira Margarida Costa Adriana Machado Ana Barros Maria Soares Bernardo Carvalho Joana Teles Silva João Varela Joana Silva Lab-Scale Optimization of <em>Aurantiochytrium</em> sp. Culture Medium for Improved Growth and DHA Production Applied Sciences thraustochytrids <i>Aurantiochytrium</i> sp. docosahexaenoic acid (DHA) culture medium optimization growth performance |
| title | Lab-Scale Optimization of <em>Aurantiochytrium</em> sp. Culture Medium for Improved Growth and DHA Production |
| title_full | Lab-Scale Optimization of <em>Aurantiochytrium</em> sp. Culture Medium for Improved Growth and DHA Production |
| title_fullStr | Lab-Scale Optimization of <em>Aurantiochytrium</em> sp. Culture Medium for Improved Growth and DHA Production |
| title_full_unstemmed | Lab-Scale Optimization of <em>Aurantiochytrium</em> sp. Culture Medium for Improved Growth and DHA Production |
| title_short | Lab-Scale Optimization of <em>Aurantiochytrium</em> sp. Culture Medium for Improved Growth and DHA Production |
| title_sort | lab scale optimization of em aurantiochytrium em sp culture medium for improved growth and dha production |
| topic | thraustochytrids <i>Aurantiochytrium</i> sp. docosahexaenoic acid (DHA) culture medium optimization growth performance |
| url | https://www.mdpi.com/2076-3417/10/7/2500 |
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