Casparian strip membrane domain proteins in Gossypium arboreum: genome-wide identification and negative regulation of lateral root growth
Abstract Background Root systems are critical for plant growth and development. The Casparian strip in root systems is involved in stress resistance and maintaining homeostasis. Casparian strip membrane domain proteins (CASPs) are responsible for the formation of Casparian strips. Results To investi...
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| Format: | Article |
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BMC
2020-05-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-020-6723-9 |
| _version_ | 1828232113748443136 |
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| author | Xiaoyang Wang Yuanming Zhang Liyuan Wang Zhaoe Pan Shoupu He Qiong Gao Baojun Chen Wenfang Gong Xiongming Du |
| author_facet | Xiaoyang Wang Yuanming Zhang Liyuan Wang Zhaoe Pan Shoupu He Qiong Gao Baojun Chen Wenfang Gong Xiongming Du |
| author_sort | Xiaoyang Wang |
| collection | DOAJ |
| description | Abstract Background Root systems are critical for plant growth and development. The Casparian strip in root systems is involved in stress resistance and maintaining homeostasis. Casparian strip membrane domain proteins (CASPs) are responsible for the formation of Casparian strips. Results To investigate the function of CASPs in cotton, we identified and characterized 48, 54, 91 and 94 CASPs from Gossypium arboreum, Gossypium raimondii, Gossypium barbadense and Gossypium hirsutum, respectively, at the genome-wide level. However, only 29 common homologous CASP genes were detected in the four Gossypium species. A collinearity analysis revealed that whole genome duplication (WGD) was the primary reason for the expansion of the genes of the CASP family in the four cotton species. However, dispersed duplication could also contribute to the expansion of the GaCASPs gene family in the ancestors of G. arboreum. Phylogenetic analysis was used to cluster a total of 85 CASP genes from G. arboreum and Arabidopsis into six distinct groups, while the genetic structure and motifs of CASPs were conserved in the same group. Most GaCASPs were expressed in diverse tissues, with the exception of that five GaCASPs (Ga08G0113, Ga08G0114, Ga08G0116, Ga08G0117 and Ga08G0118) that were highly expressed in root tissues. Analyses of the tissue and subcellular localization suggested that GaCASP27 genes (Ga08G0117) are membrane protein genes located in the root. In the GaCASP27 silenced plants and the Arabidopsis mutants, the lateral root number significantly increased. Furthermore, GaMYB36, which is related to root development was found to regulate lateral root growth by targeting GaCASP27. Conclusions This study provides a fundamental understanding of the CASP gene family in cotton and demonstrates the regulatory role of GaCASP27 on lateral root growth and development. |
| first_indexed | 2024-04-12T19:15:51Z |
| format | Article |
| id | doaj.art-91da4709cd304841a1ffafe95b82a164 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T19:15:51Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-91da4709cd304841a1ffafe95b82a1642022-12-22T03:19:44ZengBMCBMC Genomics1471-21642020-05-0121111610.1186/s12864-020-6723-9Casparian strip membrane domain proteins in Gossypium arboreum: genome-wide identification and negative regulation of lateral root growthXiaoyang Wang0Yuanming Zhang1Liyuan Wang2Zhaoe Pan3Shoupu He4Qiong Gao5Baojun Chen6Wenfang Gong7Xiongming Du8State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural SciencesCrop Information Center, College of Plant Science and Technology, Huazhong Agricultural UniversityState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural SciencesState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural SciencesState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural SciencesState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural SciencesState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural SciencesState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural SciencesState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural SciencesAbstract Background Root systems are critical for plant growth and development. The Casparian strip in root systems is involved in stress resistance and maintaining homeostasis. Casparian strip membrane domain proteins (CASPs) are responsible for the formation of Casparian strips. Results To investigate the function of CASPs in cotton, we identified and characterized 48, 54, 91 and 94 CASPs from Gossypium arboreum, Gossypium raimondii, Gossypium barbadense and Gossypium hirsutum, respectively, at the genome-wide level. However, only 29 common homologous CASP genes were detected in the four Gossypium species. A collinearity analysis revealed that whole genome duplication (WGD) was the primary reason for the expansion of the genes of the CASP family in the four cotton species. However, dispersed duplication could also contribute to the expansion of the GaCASPs gene family in the ancestors of G. arboreum. Phylogenetic analysis was used to cluster a total of 85 CASP genes from G. arboreum and Arabidopsis into six distinct groups, while the genetic structure and motifs of CASPs were conserved in the same group. Most GaCASPs were expressed in diverse tissues, with the exception of that five GaCASPs (Ga08G0113, Ga08G0114, Ga08G0116, Ga08G0117 and Ga08G0118) that were highly expressed in root tissues. Analyses of the tissue and subcellular localization suggested that GaCASP27 genes (Ga08G0117) are membrane protein genes located in the root. In the GaCASP27 silenced plants and the Arabidopsis mutants, the lateral root number significantly increased. Furthermore, GaMYB36, which is related to root development was found to regulate lateral root growth by targeting GaCASP27. Conclusions This study provides a fundamental understanding of the CASP gene family in cotton and demonstrates the regulatory role of GaCASP27 on lateral root growth and development.http://link.springer.com/article/10.1186/s12864-020-6723-9Casparian strip membrane domain proteins (CASPs)G. arboreumCollinearity analysisExpression profilesLateral root development |
| spellingShingle | Xiaoyang Wang Yuanming Zhang Liyuan Wang Zhaoe Pan Shoupu He Qiong Gao Baojun Chen Wenfang Gong Xiongming Du Casparian strip membrane domain proteins in Gossypium arboreum: genome-wide identification and negative regulation of lateral root growth BMC Genomics Casparian strip membrane domain proteins (CASPs) G. arboreum Collinearity analysis Expression profiles Lateral root development |
| title | Casparian strip membrane domain proteins in Gossypium arboreum: genome-wide identification and negative regulation of lateral root growth |
| title_full | Casparian strip membrane domain proteins in Gossypium arboreum: genome-wide identification and negative regulation of lateral root growth |
| title_fullStr | Casparian strip membrane domain proteins in Gossypium arboreum: genome-wide identification and negative regulation of lateral root growth |
| title_full_unstemmed | Casparian strip membrane domain proteins in Gossypium arboreum: genome-wide identification and negative regulation of lateral root growth |
| title_short | Casparian strip membrane domain proteins in Gossypium arboreum: genome-wide identification and negative regulation of lateral root growth |
| title_sort | casparian strip membrane domain proteins in gossypium arboreum genome wide identification and negative regulation of lateral root growth |
| topic | Casparian strip membrane domain proteins (CASPs) G. arboreum Collinearity analysis Expression profiles Lateral root development |
| url | http://link.springer.com/article/10.1186/s12864-020-6723-9 |
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