The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1
Bacteriophage JC1 is a <i>Podoviridae</i> phage with a C1 morphotype, isolated on host strain <i>Burkholderia cenocepacia</i> Van1. Phage JC1 is capable of infecting an expansive range of <i>Burkholderia cepacia</i> complex (Bcc) species. The JC1 genome exhibits s...
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MDPI AG
2022-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/5/938 |
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| author | Carly M. Davis Marta K. Ruest Jamie H. Cole Jonathan J. Dennis |
| author_facet | Carly M. Davis Marta K. Ruest Jamie H. Cole Jonathan J. Dennis |
| author_sort | Carly M. Davis |
| collection | DOAJ |
| description | Bacteriophage JC1 is a <i>Podoviridae</i> phage with a C1 morphotype, isolated on host strain <i>Burkholderia cenocepacia</i> Van1. Phage JC1 is capable of infecting an expansive range of <i>Burkholderia cepacia</i> complex (Bcc) species. The JC1 genome exhibits significant similarity and synteny to Bcep22-like phages and to many <i>Ralstonia</i> phages. The genome of JC1 was determined to be 61,182 bp in length with a 65.4% G + C content and is predicted to encode 76 proteins and 1 tRNA gene. Unlike the other <i>Lessieviruses</i>, JC1 encodes a putative helicase gene in its replication module, and it is in a unique organization not found in previously analyzed phages. The JC1 genome also harbours 3 interesting moron genes, that encode a carbon storage regulator (CsrA), an N-acetyltransferase, and a phosphoadenosine phosphosulfate (PAPS) reductase. JC1 can stably lysogenize its host Van1 and integrates into the 5′ end of the gene <i>rimO</i>. This is the first account of stable integration identified for Bcep22-like phages. JC1 has a higher global virulence index at 37 °C than at 30 °C (0.8 and 0.21, respectively); however, infection efficiency and lysogen stability are not affected by a change in temperature, and no observable temperature-sensitive switch between lytic and lysogenic lifestyle appears to exist. Although JC1 can stably lysogenize its host, it possesses some desirable characteristics for use in phage therapy. Phage JC1 has a broad host range and requires the inner core of the bacterial LPS for infection. Bacteria that mutate to evade infection by JC1 may develop a fitness disadvantage as seen in previously characterized LPS mutants lacking inner core. |
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| id | doaj.art-91dcf0cbdb824924bfb3087a8640a1d5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T01:37:35Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-91dcf0cbdb824924bfb3087a8640a1d52023-11-23T13:30:34ZengMDPI AGViruses1999-49152022-04-0114593810.3390/v14050938The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1Carly M. Davis0Marta K. Ruest1Jamie H. Cole2Jonathan J. Dennis3Department of Biological Sciences, University of Alberta, CW 405 Biological Sciences Building, Edmonton, AB T6G 2E9, CanadaDepartment of Biological Sciences, University of Alberta, CW 405 Biological Sciences Building, Edmonton, AB T6G 2E9, CanadaDepartment of Biological Sciences, University of Alberta, CW 405 Biological Sciences Building, Edmonton, AB T6G 2E9, CanadaDepartment of Biological Sciences, University of Alberta, CW 405 Biological Sciences Building, Edmonton, AB T6G 2E9, CanadaBacteriophage JC1 is a <i>Podoviridae</i> phage with a C1 morphotype, isolated on host strain <i>Burkholderia cenocepacia</i> Van1. Phage JC1 is capable of infecting an expansive range of <i>Burkholderia cepacia</i> complex (Bcc) species. The JC1 genome exhibits significant similarity and synteny to Bcep22-like phages and to many <i>Ralstonia</i> phages. The genome of JC1 was determined to be 61,182 bp in length with a 65.4% G + C content and is predicted to encode 76 proteins and 1 tRNA gene. Unlike the other <i>Lessieviruses</i>, JC1 encodes a putative helicase gene in its replication module, and it is in a unique organization not found in previously analyzed phages. The JC1 genome also harbours 3 interesting moron genes, that encode a carbon storage regulator (CsrA), an N-acetyltransferase, and a phosphoadenosine phosphosulfate (PAPS) reductase. JC1 can stably lysogenize its host Van1 and integrates into the 5′ end of the gene <i>rimO</i>. This is the first account of stable integration identified for Bcep22-like phages. JC1 has a higher global virulence index at 37 °C than at 30 °C (0.8 and 0.21, respectively); however, infection efficiency and lysogen stability are not affected by a change in temperature, and no observable temperature-sensitive switch between lytic and lysogenic lifestyle appears to exist. Although JC1 can stably lysogenize its host, it possesses some desirable characteristics for use in phage therapy. Phage JC1 has a broad host range and requires the inner core of the bacterial LPS for infection. Bacteria that mutate to evade infection by JC1 may develop a fitness disadvantage as seen in previously characterized LPS mutants lacking inner core.https://www.mdpi.com/1999-4915/14/5/938<i>Burkholderia cepacia</i> complexbacteriophagephagephage therapy |
| spellingShingle | Carly M. Davis Marta K. Ruest Jamie H. Cole Jonathan J. Dennis The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1 Viruses <i>Burkholderia cepacia</i> complex bacteriophage phage phage therapy |
| title | The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1 |
| title_full | The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1 |
| title_fullStr | The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1 |
| title_full_unstemmed | The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1 |
| title_short | The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1 |
| title_sort | isolation and characterization of a broad host range bcep22 like podovirus jc1 |
| topic | <i>Burkholderia cepacia</i> complex bacteriophage phage phage therapy |
| url | https://www.mdpi.com/1999-4915/14/5/938 |
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