The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy
Our study reported herein aims to determine whether an electromagnetic field, induced triboelectrically by a metallic cone, rotating at a frequency of 167 Hz, has an effect on the properties of the horseradish peroxidase (HRP) enzyme. Atomic force microscopy (AFM) was employed to detect even the mos...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2072-666X/13/11/1947 |
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| author | Yuri D. Ivanov Vadim Y. Tatur Ivan D. Shumov Andrey F. Kozlov Anastasia A. Valueva Irina A. Ivanova Maria O. Ershova Nina D. Ivanova Igor N. Stepanov Andrei A. Lukyanitsa Vadim S. Ziborov |
| author_facet | Yuri D. Ivanov Vadim Y. Tatur Ivan D. Shumov Andrey F. Kozlov Anastasia A. Valueva Irina A. Ivanova Maria O. Ershova Nina D. Ivanova Igor N. Stepanov Andrei A. Lukyanitsa Vadim S. Ziborov |
| author_sort | Yuri D. Ivanov |
| collection | DOAJ |
| description | Our study reported herein aims to determine whether an electromagnetic field, induced triboelectrically by a metallic cone, rotating at a frequency of 167 Hz, has an effect on the properties of the horseradish peroxidase (HRP) enzyme. Atomic force microscopy (AFM) was employed to detect even the most subtle effects on single enzyme molecules. In parallel, a macroscopic method (spectrophotometry) was used to reveal whether the enzymatic activity of HRP in solution was affected. An aqueous solution of the enzyme was incubated at a distance of 2 cm from the rotating cone. The experiments were performed at various incubation times. The control experiments were performed with a non-rotating cone. The incubation of the HRP solution was found to cause the disaggregation of the enzyme. At longer incubation times, this disaggregation was found to be accompanied by the formation of higher-order aggregates; however, no change in the HRP enzymatic activity was observed. The results of our experiments could be of interest in the development of enzyme-based biosensors with rotating elements such as stirrers. Additionally, the results obtained herein are important for the correct interpretation of data obtained with such biosensors. |
| first_indexed | 2024-03-09T18:50:06Z |
| format | Article |
| id | doaj.art-91de87167a5f4245b52fd96d857c495c |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T18:50:06Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-91de87167a5f4245b52fd96d857c495c2023-11-24T05:55:39ZengMDPI AGMicromachines2072-666X2022-11-011311194710.3390/mi13111947The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force MicroscopyYuri D. Ivanov0Vadim Y. Tatur1Ivan D. Shumov2Andrey F. Kozlov3Anastasia A. Valueva4Irina A. Ivanova5Maria O. Ershova6Nina D. Ivanova7Igor N. Stepanov8Andrei A. Lukyanitsa9Vadim S. Ziborov10Institute of Biomedical Chemistry, Pogodinskaya Str., 10 Build. 8, 119121 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10 Build. 8, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10 Build. 8, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10 Build. 8, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10 Build. 8, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10 Build. 8, 119121 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya Str., 10 Build. 8, 119121 Moscow, RussiaOur study reported herein aims to determine whether an electromagnetic field, induced triboelectrically by a metallic cone, rotating at a frequency of 167 Hz, has an effect on the properties of the horseradish peroxidase (HRP) enzyme. Atomic force microscopy (AFM) was employed to detect even the most subtle effects on single enzyme molecules. In parallel, a macroscopic method (spectrophotometry) was used to reveal whether the enzymatic activity of HRP in solution was affected. An aqueous solution of the enzyme was incubated at a distance of 2 cm from the rotating cone. The experiments were performed at various incubation times. The control experiments were performed with a non-rotating cone. The incubation of the HRP solution was found to cause the disaggregation of the enzyme. At longer incubation times, this disaggregation was found to be accompanied by the formation of higher-order aggregates; however, no change in the HRP enzymatic activity was observed. The results of our experiments could be of interest in the development of enzyme-based biosensors with rotating elements such as stirrers. Additionally, the results obtained herein are important for the correct interpretation of data obtained with such biosensors.https://www.mdpi.com/2072-666X/13/11/1947horseradish peroxidaseenzyme aggregationatomic force microscopytriboelectric effectenzyme-based biosensor |
| spellingShingle | Yuri D. Ivanov Vadim Y. Tatur Ivan D. Shumov Andrey F. Kozlov Anastasia A. Valueva Irina A. Ivanova Maria O. Ershova Nina D. Ivanova Igor N. Stepanov Andrei A. Lukyanitsa Vadim S. Ziborov The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy Micromachines horseradish peroxidase enzyme aggregation atomic force microscopy triboelectric effect enzyme-based biosensor |
| title | The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy |
| title_full | The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy |
| title_fullStr | The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy |
| title_full_unstemmed | The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy |
| title_short | The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy |
| title_sort | effect of a rotating cone on horseradish peroxidase aggregation on mica revealed by atomic force microscopy |
| topic | horseradish peroxidase enzyme aggregation atomic force microscopy triboelectric effect enzyme-based biosensor |
| url | https://www.mdpi.com/2072-666X/13/11/1947 |
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