Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication
This paper discusses the perspectives of applications of the detonation nanodiamonds in biotechnology. An effective separation of proteins from complex protein mixtures by using of nanodiamonds is shown on the example of purification of recombinant luciferase from the extracts of E. coli bacterial c...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Siberian Federal University
2010-12-01
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| Series: | Журнал Сибирского федерального университета: Серия Биология |
| Subjects: | |
| Online Access: | http://elib.sfu-kras.ru/bitstream/2311/2249/1/Ronzhin.pdf |
| _version_ | 1818175871619432448 |
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| author | Nikita O. Ronzhin Konstantin A. Kharin Alexey P. Puzyr Vladimir S. Bondar |
| author_facet | Nikita O. Ronzhin Konstantin A. Kharin Alexey P. Puzyr Vladimir S. Bondar |
| author_sort | Nikita O. Ronzhin |
| collection | DOAJ |
| description | This paper discusses the perspectives of applications of the detonation nanodiamonds in biotechnology. An effective separation of proteins from complex protein mixtures by using of nanodiamonds is shown on the example of purification of recombinant luciferase from the extracts of E. coli bacterial cells: the process requires minimum equipment and time; one technological cycle yields 40-45 % of desired product with high-quality (according to the SDS-PAGE). The application of nanodiamonds for design of indicating systems is demonstrated on the example of a biosensor system based on the nanodiamondluciferase complex and inert polymeric matrix. |
| first_indexed | 2024-12-11T20:07:11Z |
| format | Article |
| id | doaj.art-91debe81a25a4b48a6ab5904c04124f4 |
| institution | Directory Open Access Journal |
| issn | 1997-1389 2313-5530 |
| language | English |
| last_indexed | 2024-12-11T20:07:11Z |
| publishDate | 2010-12-01 |
| publisher | Siberian Federal University |
| record_format | Article |
| series | Журнал Сибирского федерального университета: Серия Биология |
| spelling | doaj.art-91debe81a25a4b48a6ab5904c04124f42022-12-22T00:52:22ZengSiberian Federal UniversityЖурнал Сибирского федерального университета: Серия Биология1997-13892313-55302010-12-0134418433http://elib.sfu-kras.ru/handle/2311/2249Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of IndicationNikita O. Ronzhin0Konstantin A. Kharin1Alexey P. Puzyr2Vladimir S. Bondar3Siberian Federal University , 79 Svobodny, Krasnoyarsk, 660041 RussiaSiberian Federal University , 79 Svobodny, Krasnoyarsk, 660041 RussiaInstitute of Biophysics SB RAS , 50/50 Akademgorodok, Krasnoyarsk, 660036 RussiaSiberian Federal University Institute of Biophysics SB RAS , 79 Svobodny, Krasnoyarsk, 660041 Russia 50/50 Akademgorodok, Krasnoyarsk, 660036 RussiaThis paper discusses the perspectives of applications of the detonation nanodiamonds in biotechnology. An effective separation of proteins from complex protein mixtures by using of nanodiamonds is shown on the example of purification of recombinant luciferase from the extracts of E. coli bacterial cells: the process requires minimum equipment and time; one technological cycle yields 40-45 % of desired product with high-quality (according to the SDS-PAGE). The application of nanodiamonds for design of indicating systems is demonstrated on the example of a biosensor system based on the nanodiamondluciferase complex and inert polymeric matrix.http://elib.sfu-kras.ru/bitstream/2311/2249/1/Ronzhin.pdfmodified detonation nanodiamondsrecombinant luciferaseprotein purificationbiotechnologybioluminescencetest-system |
| spellingShingle | Nikita O. Ronzhin Konstantin A. Kharin Alexey P. Puzyr Vladimir S. Bondar Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication Журнал Сибирского федерального университета: Серия Биология modified detonation nanodiamonds recombinant luciferase protein purification biotechnology bioluminescence test-system |
| title | Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication |
| title_full | Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication |
| title_fullStr | Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication |
| title_full_unstemmed | Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication |
| title_short | Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication |
| title_sort | nanodiamonds in biotechnology application for purification of proteins and test systems of indication |
| topic | modified detonation nanodiamonds recombinant luciferase protein purification biotechnology bioluminescence test-system |
| url | http://elib.sfu-kras.ru/bitstream/2311/2249/1/Ronzhin.pdf |
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