CyTOF protocol for immune monitoring of solid tumors from mouse models

CyTOF protocol for immune monitoring of solid tumors from mouse models

Summary: Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples a...

Cur síos iomlán

Sonraí bibleagrafaíochta
Príomhchruthaitheoirí:	Sarah M. Shin, Alexei Hernandez, Erin Coyne, Kabeer Munjal, Nicole E. Gross, Soren Charmsaz, Xuan Yuan, Hongqui Yang, Won Jin Ho
Formáid:	Alt
Teanga:	English
Foilsithe / Cruthaithe:	Elsevier 2023-03-01
Sraith:	STAR Protocols
Ábhair:	Flow Cytometry/Mass Cytometry Cancer Immunology Antibody
Rochtain ar líne:	http://www.sciencedirect.com/science/article/pii/S2666166722008292

Míreanna comhchosúla

CyTOF® for the Masses
de réir: Akshay Iyer, et al.
Foilsithe / Cruthaithe: (2022-04-01)

CyTOF mass cytometry analysis of human memory CD4+ T cells and memory B cells
de réir: Lisa J. Ioannidis, et al.
Foilsithe / Cruthaithe: (2022-06-01)

Validation of CyTOF Against Flow Cytometry for Immunological Studies and Monitoring of Human Cancer Clinical Trials
de réir: Ramy Gadalla, et al.
Foilsithe / Cruthaithe: (2019-05-01)

CyTOF Analysis Reveals a Distinct Immunosuppressive Microenvironment in IDH Mutant Anaplastic Gliomas
de réir: Weilun Fu, et al.
Foilsithe / Cruthaithe: (2021-02-01)

CyTOF analysis identifies unusual immune cells in urine of BCG-treated bladder cancer patients
de réir: Eva Castellano, et al.
Foilsithe / Cruthaithe: (2022-09-01)

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
de réir: Ioannis Skordos, et al.
Foilsithe / Cruthaithe: (2021-03-01)

Identification of clinically relevant subsets CD39+PD-1+CD8+ T cells and CD39+ regulatory T cells in intrahepatic cholangiocarcinoma using single-cell CyTOF
de réir: Qi-Wei Zhang, et al.
Foilsithe / Cruthaithe: (2024-06-01)

Circulation immune cell landscape in canonical pathogenesis of colorectal adenocarcinoma by CyTOF analysis
de réir: Xiang-Xing Kong, et al.
Foilsithe / Cruthaithe: (2024-03-01)

Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry
de réir: Jinzhi Duan, et al.
Foilsithe / Cruthaithe: (2023-09-01)

Subsets of Tissue CD4 T Cells Display Different Susceptibilities to HIV Infection and Death: Analysis by CyTOF and Single Cell RNA-seq
de réir: Xiaoyu Luo, et al.
Foilsithe / Cruthaithe: (2022-06-01)

Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro
de réir: Simon Wabitsch, et al.
Foilsithe / Cruthaithe: (2021-06-01)

Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages
de réir: Ignazio Antignano, et al.
Foilsithe / Cruthaithe: (2023-12-01)

Evaluation of mTORC1 signaling in mouse atherosclerotic macrophages by flow cytometry and immunofluorescence
de réir: Xiangyu Zhang, et al.
Foilsithe / Cruthaithe: (2022-12-01)

Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
de réir: José Ángel Nicolás-Ávila, et al.
Foilsithe / Cruthaithe: (2021-03-01)

Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence
de réir: Tomasz Ahrends, et al.
Foilsithe / Cruthaithe: (2022-03-01)

Phenotypic and functional alteration of CD45+ immune cells in the decidua of preeclampsia patients analyzed by mass cytometry (CyTOF)
de réir: Min Fu, et al.
Foilsithe / Cruthaithe: (2023-01-01)

High-yield enrichment of mouse small intestine intraepithelial lymphocytes by immunomagnetic depletion of EpCAM+ cells
de réir: Praveen Prakhar, et al.
Foilsithe / Cruthaithe: (2022-03-01)

Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease
de réir: Calvin Krollmann, et al.
Foilsithe / Cruthaithe: (2022-06-01)

Mass cytometry immunostaining protocol for multiplexing clinical samples
de réir: Ramy Gadalla, et al.
Foilsithe / Cruthaithe: (2022-09-01)

High-throughput mass cytometry staining for deep phenotyping of human natural killer cells
de réir: Amira Ben Amara, et al.
Foilsithe / Cruthaithe: (2022-12-01)

In vitro flow cytometry assay to assess primary human and mouse macrophage phagocytosis of live cells
de réir: Samantha Y. Liu, et al.
Foilsithe / Cruthaithe: (2023-06-01)

Flow cytometry protocol to quantify immune cells in the separated epithelium and stroma of herpes simplex virus-1-infected mouse cornea
de réir: Mizumi Setia, et al.
Foilsithe / Cruthaithe: (2023-03-01)

Removing unwanted variation with CytofRUV to integrate multiple CyTOF datasets
de réir: Marie Trussart, et al.
Foilsithe / Cruthaithe: (2020-09-01)

Characterization of immune populations in the tumor microenvironment of diffuse midline glioma orthotopic mouse models by flow cytometry
de réir: Iker Ausejo-Mauleon, et al.
Foilsithe / Cruthaithe: (2024-03-01)

Isolation and analyses of lamina propria lymphocytes from mouse intestines
de réir: Eunha Kim, et al.
Foilsithe / Cruthaithe: (2022-06-01)

Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens
de réir: Leonie M. Weskamm, et al.
Foilsithe / Cruthaithe: (2022-12-01)

Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining lymph nodes of mice using flow cytometry
de réir: Naoya Tatsumi, et al.
Foilsithe / Cruthaithe: (2022-12-01)

Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
de réir: Xiuxing Liu, et al.
Foilsithe / Cruthaithe: (2022-06-01)

Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
de réir: Keiko Sakamoto, et al.
Foilsithe / Cruthaithe: (2022-03-01)

An optimized protocol for the isolation of rare stromal cell populations from the mouse spleen
de réir: Yannick O. Alexandre, et al.
Foilsithe / Cruthaithe: (2022-12-01)

A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
de réir: Nicolas Jacquelot, et al.
Foilsithe / Cruthaithe: (2022-09-01)

In vitro and ex vivo evaluation of tumor-derived exosome-induced dendritic cell dysfunction in mouse
de réir: Wenfeng Zeng, et al.
Foilsithe / Cruthaithe: (2021-03-01)

Protocol for analysis of mouse neutrophil NETosis by flow cytometry
de réir: Cassandra J. McGill, et al.
Foilsithe / Cruthaithe: (2021-12-01)

Cytomulate: accurate and efficient simulation of CyTOF data
de réir: Yuqiu Yang, et al.
Foilsithe / Cruthaithe: (2023-11-01)

Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
de réir: Annie Roussel-Queval, et al.
Foilsithe / Cruthaithe: (2023-03-01)

Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouse
de réir: Sai Sakktee Krisna, et al.
Foilsithe / Cruthaithe: (2021-09-01)

FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses
de réir: Camilla Tiezzi, et al.
Foilsithe / Cruthaithe: (2023-12-01)

High-throughput interrogation of immune responses using the Human Immune Profiling Pipeline
de réir: Guanning Wang, et al.
Foilsithe / Cruthaithe: (2023-06-01)

Protocol for identification and computational analysis of human natural killer cells using flow cytometry and R
de réir: Kyle Kroll, et al.
Foilsithe / Cruthaithe: (2023-03-01)

Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline
de réir: Paulina Rybakowska, et al.
Foilsithe / Cruthaithe: (2022-12-01)