Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring.
Numerous studies have demonstrated that endocrine-disrupting compounds (EDC) are a possible cause of male reproductive organ malfunction and malformation. Cypermethrin (CYP) is a widely used synthetic pyrethroid and a potential EDC. This study aimed to examine the effects of perinatal exposure to lo...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4014553?pdf=render |
| _version_ | 1819231725345046528 |
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| author | Chaobin Huang Xiangdong Li |
| author_facet | Chaobin Huang Xiangdong Li |
| author_sort | Chaobin Huang |
| collection | DOAJ |
| description | Numerous studies have demonstrated that endocrine-disrupting compounds (EDC) are a possible cause of male reproductive organ malfunction and malformation. Cypermethrin (CYP) is a widely used synthetic pyrethroid and a potential EDC. This study aimed to examine the effects of perinatal exposure to low-dose CYP on the development and function of the offspring testes. Pregnant mice were intragastrically administered 0.12 to 12 mg/kg/day CYP from embryonic day 0.5 (E0.5) to weaning (PD21.5, postnatal day 21.5). Maternal exposure to 0.12, 1.2, and 12 mg/kg/day CYP affected the body and organ weight of the offspring. Exposure of CYP led to a dose-dependent decrease in the male-to-female sex ratio. A histopathological analysis revealed a thinner seminiferous epithelium layer at PD21.5, interstitial hyperplasia at PD45.5, and germ cell vacuolization at PD90.5 in the 12 mg/kg/day CYP group. The TUNEL assay results revealed increased germ cell apoptosis in the 12 mg/kg/day CYP group. The serum testosterone (T) level decreased, whereas the estradiol level increased with age in the 1.2 and 12 mg/kg/day CYP groups. The RT-PCR analysis demonstrated decreased expression of T production-related, mitosis-related, and meiosis-related genes in the 1.2 and 12 mg/kg/day CYP groups. The in vitro experimental results demonstrated reduced expression of steroidogenesis genes and decreased T levels. It is concluded that perinatal exposure to low-dose CYP affects testes development and function in adults. |
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| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T11:49:32Z |
| publishDate | 2014-01-01 |
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| spelling | doaj.art-91e0c0e5dbba4bf5bf1ce1538421ad542022-12-21T17:48:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9678110.1371/journal.pone.0096781Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring.Chaobin HuangXiangdong LiNumerous studies have demonstrated that endocrine-disrupting compounds (EDC) are a possible cause of male reproductive organ malfunction and malformation. Cypermethrin (CYP) is a widely used synthetic pyrethroid and a potential EDC. This study aimed to examine the effects of perinatal exposure to low-dose CYP on the development and function of the offspring testes. Pregnant mice were intragastrically administered 0.12 to 12 mg/kg/day CYP from embryonic day 0.5 (E0.5) to weaning (PD21.5, postnatal day 21.5). Maternal exposure to 0.12, 1.2, and 12 mg/kg/day CYP affected the body and organ weight of the offspring. Exposure of CYP led to a dose-dependent decrease in the male-to-female sex ratio. A histopathological analysis revealed a thinner seminiferous epithelium layer at PD21.5, interstitial hyperplasia at PD45.5, and germ cell vacuolization at PD90.5 in the 12 mg/kg/day CYP group. The TUNEL assay results revealed increased germ cell apoptosis in the 12 mg/kg/day CYP group. The serum testosterone (T) level decreased, whereas the estradiol level increased with age in the 1.2 and 12 mg/kg/day CYP groups. The RT-PCR analysis demonstrated decreased expression of T production-related, mitosis-related, and meiosis-related genes in the 1.2 and 12 mg/kg/day CYP groups. The in vitro experimental results demonstrated reduced expression of steroidogenesis genes and decreased T levels. It is concluded that perinatal exposure to low-dose CYP affects testes development and function in adults.http://europepmc.org/articles/PMC4014553?pdf=render |
| spellingShingle | Chaobin Huang Xiangdong Li Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring. PLoS ONE |
| title | Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring. |
| title_full | Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring. |
| title_fullStr | Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring. |
| title_full_unstemmed | Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring. |
| title_short | Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring. |
| title_sort | maternal cypermethrin exposure during the perinatal period impairs testicular development in c57bl male offspring |
| url | http://europepmc.org/articles/PMC4014553?pdf=render |
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