Dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting HuR/Pim1 axis via the induction of miR-16-5p level
Dioscin (DS) is a natural compound with inhibitory effects on multiple cancer types and we explored the mechanism driving the antagonizing effect of DS on osteosarcoma (OS). OS cell lines were treated with DS and microRNAs (miRs) responding to DS treatment were determined. Effects of DS on viability...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622003292 |
| _version_ | 1811197014932717568 |
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| author | Bo Jiang Xinsheng Fu Qichuan Zhang Yunfei Du |
| author_facet | Bo Jiang Xinsheng Fu Qichuan Zhang Yunfei Du |
| author_sort | Bo Jiang |
| collection | DOAJ |
| description | Dioscin (DS) is a natural compound with inhibitory effects on multiple cancer types and we explored the mechanism driving the antagonizing effect of DS on osteosarcoma (OS). OS cell lines were treated with DS and microRNAs (miRs) responding to DS treatment were determined. Effects of DS on viability, apoptosis, metastasis, and miR-16-5p/HuR/Pim1 pathway activity were assessed. MiR-16-5p was inhibited before DS treatment to confirm its central role in anti-OS effects of DS. The conclusion derived from in vitro assays was verified with a mouse model. DS suppressed viability, invasion and migration, while induced apoptosis in OS cells. Moreover, the compound also induced the expression of miR-16-5p, contributing to the inhibition of HuR/Pim1 pathway. The inhibited expression of miR-16-5p counteracted the anti-OS function of DS, which was also validated in mice. Anti-OS effects of DS depended on the induction of miR-16-5p, making the compound a promising adjuvant agent for treating OS. |
| first_indexed | 2024-04-12T01:08:01Z |
| format | Article |
| id | doaj.art-91e26598c8cd4207be2adb29db357a30 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-12T01:08:01Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-91e26598c8cd4207be2adb29db357a302022-12-22T03:54:11ZengElsevierJournal of Functional Foods1756-46462022-11-0198105259Dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting HuR/Pim1 axis via the induction of miR-16-5p levelBo Jiang0Xinsheng Fu1Qichuan Zhang2Yunfei Du3Corresponding author at: Department of Orthopedics, Xinxiang Central Hospital, No.56 Jinsui Road, Xinxiang 453000, Henan, China.; Department of Orthopedics, Xinxiang Central Hospital, Xinxiang 453000, Henan, ChinaDepartment of Orthopedics, Xinxiang Central Hospital, Xinxiang 453000, Henan, ChinaDepartment of Orthopedics, Xinxiang Central Hospital, Xinxiang 453000, Henan, ChinaDepartment of Orthopedics, Xinxiang Central Hospital, Xinxiang 453000, Henan, ChinaDioscin (DS) is a natural compound with inhibitory effects on multiple cancer types and we explored the mechanism driving the antagonizing effect of DS on osteosarcoma (OS). OS cell lines were treated with DS and microRNAs (miRs) responding to DS treatment were determined. Effects of DS on viability, apoptosis, metastasis, and miR-16-5p/HuR/Pim1 pathway activity were assessed. MiR-16-5p was inhibited before DS treatment to confirm its central role in anti-OS effects of DS. The conclusion derived from in vitro assays was verified with a mouse model. DS suppressed viability, invasion and migration, while induced apoptosis in OS cells. Moreover, the compound also induced the expression of miR-16-5p, contributing to the inhibition of HuR/Pim1 pathway. The inhibited expression of miR-16-5p counteracted the anti-OS function of DS, which was also validated in mice. Anti-OS effects of DS depended on the induction of miR-16-5p, making the compound a promising adjuvant agent for treating OS.http://www.sciencedirect.com/science/article/pii/S1756464622003292DioscinmiR-16-5pOsteosarcomaHuRPim1 |
| spellingShingle | Bo Jiang Xinsheng Fu Qichuan Zhang Yunfei Du Dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting HuR/Pim1 axis via the induction of miR-16-5p level Journal of Functional Foods Dioscin miR-16-5p Osteosarcoma HuR Pim1 |
| title | Dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting HuR/Pim1 axis via the induction of miR-16-5p level |
| title_full | Dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting HuR/Pim1 axis via the induction of miR-16-5p level |
| title_fullStr | Dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting HuR/Pim1 axis via the induction of miR-16-5p level |
| title_full_unstemmed | Dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting HuR/Pim1 axis via the induction of miR-16-5p level |
| title_short | Dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting HuR/Pim1 axis via the induction of miR-16-5p level |
| title_sort | dioscin suppresses proliferation and metastasis of osteosarcoma by inhibiting hur pim1 axis via the induction of mir 16 5p level |
| topic | Dioscin miR-16-5p Osteosarcoma HuR Pim1 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622003292 |
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