Investigation of <it>Chlamydophila </it>spp. in dairy cows with reproductive disorders
<p>Abstract</p> <p>Background</p> <p>Reports worldwide indicate high prevalence of <it>Chlamydophila </it>spp. infection in cattle. To assess the prevalence in Sweden, 525 cows in 70 dairy herds with reproductive disorders was investigated.</p> <p&g...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-09-01
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| Series: | Acta Veterinaria Scandinavica |
| Online Access: | http://www.actavetscand.com/content/50/1/39 |
| _version_ | 1818835743234064384 |
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| author | Niskanen Rauni Johansson Karl-Erik Englund Stina Björkman Camilla Godin Ann-Charlotte Alenius Stefan |
| author_facet | Niskanen Rauni Johansson Karl-Erik Englund Stina Björkman Camilla Godin Ann-Charlotte Alenius Stefan |
| author_sort | Niskanen Rauni |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Reports worldwide indicate high prevalence of <it>Chlamydophila </it>spp. infection in cattle. To assess the prevalence in Sweden, 525 cows in 70 dairy herds with reproductive disorders was investigated.</p> <p>Methods</p> <p>To detect antibodies two commercially available kits were used. Moreover, 107 specimens, including vaginal swabs, organ tissues and milk were analysed by Polymerase Chain Reaction (PCR).</p> <p>Results</p> <p>Two (0.4%) cows were seropositive in the Pourquier <it>Cp. abortus </it>ELISA. The seroprevalence with the Chekit ELISA was 28% with no difference between cases and controls. Five specimens were positive in real-time PCR and further analysed by nested PCR. <it>Cp. pecorum </it>was confirmed by partial <it>omp1 </it>DNA sequencing of the nested PCR product of vaginal swabs from control cows.</p> <p>Conclusion</p> <p>The results suggest that <it>Cp. abortus </it>infection is absent or rare in Swedish cows whereas <it>Cp. pecorum </it>is probably more spread. They also suggest that <it>Chlamydophila </it>spp. are not related to reproduction disorders in Swedish cattle.</p> |
| first_indexed | 2024-12-19T02:55:34Z |
| format | Article |
| id | doaj.art-91e4b3d5081d4df5be7395c1ed3c5bae |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-12-19T02:55:34Z |
| publishDate | 2008-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-91e4b3d5081d4df5be7395c1ed3c5bae2022-12-21T20:38:21ZengBMCActa Veterinaria Scandinavica1751-01472008-09-015013910.1186/1751-0147-50-39Investigation of <it>Chlamydophila </it>spp. in dairy cows with reproductive disordersNiskanen RauniJohansson Karl-ErikEnglund StinaBjörkman CamillaGodin Ann-CharlotteAlenius Stefan<p>Abstract</p> <p>Background</p> <p>Reports worldwide indicate high prevalence of <it>Chlamydophila </it>spp. infection in cattle. To assess the prevalence in Sweden, 525 cows in 70 dairy herds with reproductive disorders was investigated.</p> <p>Methods</p> <p>To detect antibodies two commercially available kits were used. Moreover, 107 specimens, including vaginal swabs, organ tissues and milk were analysed by Polymerase Chain Reaction (PCR).</p> <p>Results</p> <p>Two (0.4%) cows were seropositive in the Pourquier <it>Cp. abortus </it>ELISA. The seroprevalence with the Chekit ELISA was 28% with no difference between cases and controls. Five specimens were positive in real-time PCR and further analysed by nested PCR. <it>Cp. pecorum </it>was confirmed by partial <it>omp1 </it>DNA sequencing of the nested PCR product of vaginal swabs from control cows.</p> <p>Conclusion</p> <p>The results suggest that <it>Cp. abortus </it>infection is absent or rare in Swedish cows whereas <it>Cp. pecorum </it>is probably more spread. They also suggest that <it>Chlamydophila </it>spp. are not related to reproduction disorders in Swedish cattle.</p>http://www.actavetscand.com/content/50/1/39 |
| spellingShingle | Niskanen Rauni Johansson Karl-Erik Englund Stina Björkman Camilla Godin Ann-Charlotte Alenius Stefan Investigation of <it>Chlamydophila </it>spp. in dairy cows with reproductive disorders Acta Veterinaria Scandinavica |
| title | Investigation of <it>Chlamydophila </it>spp. in dairy cows with reproductive disorders |
| title_full | Investigation of <it>Chlamydophila </it>spp. in dairy cows with reproductive disorders |
| title_fullStr | Investigation of <it>Chlamydophila </it>spp. in dairy cows with reproductive disorders |
| title_full_unstemmed | Investigation of <it>Chlamydophila </it>spp. in dairy cows with reproductive disorders |
| title_short | Investigation of <it>Chlamydophila </it>spp. in dairy cows with reproductive disorders |
| title_sort | investigation of it chlamydophila it spp in dairy cows with reproductive disorders |
| url | http://www.actavetscand.com/content/50/1/39 |
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