Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers
Abstract Background Breeding programs need to work with magnitudes of genetic variation in order to achieve the best results. In this study, we have estimated the genetic diversity and evaluated the field performance of 18 faba bean genotypes from different origins, during the 2014–2015 and 2015–201...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-07-01
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| Series: | Bulletin of the National Research Centre |
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| Online Access: | http://link.springer.com/article/10.1186/s42269-019-0145-3 |
| _version_ | 1818506275204366336 |
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| author | Adel Ahmed Mohamed Elshafei Mohamed Abd Elaziz Amer Magda Aly Mahmoud Elenany Ahmed Gamal Abd Elhameed Helal |
| author_facet | Adel Ahmed Mohamed Elshafei Mohamed Abd Elaziz Amer Magda Aly Mahmoud Elenany Ahmed Gamal Abd Elhameed Helal |
| author_sort | Adel Ahmed Mohamed Elshafei |
| collection | DOAJ |
| description | Abstract Background Breeding programs need to work with magnitudes of genetic variation in order to achieve the best results. In this study, we have estimated the genetic diversity and evaluated the field performance of 18 faba bean genotypes from different origins, during the 2014–2015 and 2015–2016 growing seasons. Results The field evaluations revealed that the faba bean genotypes showed enough variation for their agronomic thoughtful parameters. ALB was noted as being the genotype that was the tallest (126.25 cm), but the superlative genotypes were L251 and L252 for a high number of seeds per plant (72.25) and the maximum seed yield per plant (56.40 g). The estimations of the genetic diversity between all of the faba bean genotypes were done using eight sequence-related amplified polymorphism (SRAP)-combined primers. The number of different alleles amplified for each polymorphic marker, ranged from 2 to 9, was observed by SRAP genotyping, with polymorphic information contents (PIC) between 0.445and 0.896, and discrimination power (DP) with an average of 0.125 per allele. Conclusions Across both years, the best grain yields were gathered from the L25, L252, and L182 genotypes, in descending order, whereas the ALB genotype had the lowest grain yield in comparison with the other genotypes. The cluster analysis of faba bean genotypes was generally based on not only the origin but also the genetic background. |
| first_indexed | 2024-12-10T22:02:22Z |
| format | Article |
| id | doaj.art-91e67c95939f48a785fbef8c1ac2d85d |
| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-12-10T22:02:22Z |
| publishDate | 2019-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bulletin of the National Research Centre |
| spelling | doaj.art-91e67c95939f48a785fbef8c1ac2d85d2022-12-22T01:31:52ZengSpringerOpenBulletin of the National Research Centre2522-83072019-07-0143111010.1186/s42269-019-0145-3Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markersAdel Ahmed Mohamed Elshafei0Mohamed Abd Elaziz Amer1Magda Aly Mahmoud Elenany2Ahmed Gamal Abd Elhameed Helal3Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC)Genetic Resources Research Department, Field Crops Research Institute, Agriculture Research CenterGenetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC)Food Legume Crops Research Department, Field Crops Research Institute, Agriculture Research CenterAbstract Background Breeding programs need to work with magnitudes of genetic variation in order to achieve the best results. In this study, we have estimated the genetic diversity and evaluated the field performance of 18 faba bean genotypes from different origins, during the 2014–2015 and 2015–2016 growing seasons. Results The field evaluations revealed that the faba bean genotypes showed enough variation for their agronomic thoughtful parameters. ALB was noted as being the genotype that was the tallest (126.25 cm), but the superlative genotypes were L251 and L252 for a high number of seeds per plant (72.25) and the maximum seed yield per plant (56.40 g). The estimations of the genetic diversity between all of the faba bean genotypes were done using eight sequence-related amplified polymorphism (SRAP)-combined primers. The number of different alleles amplified for each polymorphic marker, ranged from 2 to 9, was observed by SRAP genotyping, with polymorphic information contents (PIC) between 0.445and 0.896, and discrimination power (DP) with an average of 0.125 per allele. Conclusions Across both years, the best grain yields were gathered from the L25, L252, and L182 genotypes, in descending order, whereas the ALB genotype had the lowest grain yield in comparison with the other genotypes. The cluster analysis of faba bean genotypes was generally based on not only the origin but also the genetic background.http://link.springer.com/article/10.1186/s42269-019-0145-3Genetic diversityAgronomic traitsFaba beanSRAPPIC |
| spellingShingle | Adel Ahmed Mohamed Elshafei Mohamed Abd Elaziz Amer Magda Aly Mahmoud Elenany Ahmed Gamal Abd Elhameed Helal Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers Bulletin of the National Research Centre Genetic diversity Agronomic traits Faba bean SRAP PIC |
| title | Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers |
| title_full | Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers |
| title_fullStr | Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers |
| title_full_unstemmed | Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers |
| title_short | Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers |
| title_sort | evaluation of the genetic variability of faba bean vicia faba l genotypes using agronomic traits and molecular markers |
| topic | Genetic diversity Agronomic traits Faba bean SRAP PIC |
| url | http://link.springer.com/article/10.1186/s42269-019-0145-3 |
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