Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila
The hematopoietic system of Drosophila is a powerful genetic model for studying hematopoiesis, and vesicle trafficking is important for signal transduction during various developmental processes; however, its interaction with hematopoiesis is currently largely unknown. In this article, we selected t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/60870 |
| _version_ | 1797997571704094720 |
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| author | Shichao Yu Fangzhou Luo Li Hua Jin |
| author_facet | Shichao Yu Fangzhou Luo Li Hua Jin |
| author_sort | Shichao Yu |
| collection | DOAJ |
| description | The hematopoietic system of Drosophila is a powerful genetic model for studying hematopoiesis, and vesicle trafficking is important for signal transduction during various developmental processes; however, its interaction with hematopoiesis is currently largely unknown. In this article, we selected three endosome markers, Rab5, Rab7, and Rab11, that play a key role in membrane trafficking and determined whether they participate in hematopoiesis. Inhibiting Rab5 or Rab11 in hemocytes or the cortical zone (CZ) significantly induced cell overproliferation and lamellocyte formation in circulating hemocytes and lymph glands and disrupted blood cell progenitor maintenance. Lamellocyte formation involves the JNK, Toll, and Ras/EGFR signaling pathways. Notably, lamellocyte formation was also associated with JNK-dependent autophagy. In conclusion, we identified Rab5 and Rab11 as novel regulators of hematopoiesis, and our results advance the understanding of the mechanisms underlying the maintenance of hematopoietic homeostasis as well as the pathology of blood disorders such as leukemia. |
| first_indexed | 2024-04-11T10:34:46Z |
| format | Article |
| id | doaj.art-91ebf51f21064bbf91dd8510101ef526 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:34:46Z |
| publishDate | 2021-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-91ebf51f21064bbf91dd8510101ef5262022-12-22T04:29:19ZengeLife Sciences Publications LtdeLife2050-084X2021-02-011010.7554/eLife.60870Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in DrosophilaShichao Yu0Fangzhou Luo1Li Hua Jin2https://orcid.org/0000-0001-5912-9800Department of Genetics, College of Life Sciences, Northeast Forestry University, Harbin, ChinaDepartment of Genetics, College of Life Sciences, Northeast Forestry University, Harbin, ChinaDepartment of Genetics, College of Life Sciences, Northeast Forestry University, Harbin, ChinaThe hematopoietic system of Drosophila is a powerful genetic model for studying hematopoiesis, and vesicle trafficking is important for signal transduction during various developmental processes; however, its interaction with hematopoiesis is currently largely unknown. In this article, we selected three endosome markers, Rab5, Rab7, and Rab11, that play a key role in membrane trafficking and determined whether they participate in hematopoiesis. Inhibiting Rab5 or Rab11 in hemocytes or the cortical zone (CZ) significantly induced cell overproliferation and lamellocyte formation in circulating hemocytes and lymph glands and disrupted blood cell progenitor maintenance. Lamellocyte formation involves the JNK, Toll, and Ras/EGFR signaling pathways. Notably, lamellocyte formation was also associated with JNK-dependent autophagy. In conclusion, we identified Rab5 and Rab11 as novel regulators of hematopoiesis, and our results advance the understanding of the mechanisms underlying the maintenance of hematopoietic homeostasis as well as the pathology of blood disorders such as leukemia.https://elifesciences.org/articles/60870vesicle traffickingRab5Rab11hematopoiesislymph glandhemocyte |
| spellingShingle | Shichao Yu Fangzhou Luo Li Hua Jin Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila eLife vesicle trafficking Rab5 Rab11 hematopoiesis lymph gland hemocyte |
| title | Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila |
| title_full | Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila |
| title_fullStr | Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila |
| title_full_unstemmed | Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila |
| title_short | Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila |
| title_sort | rab5 and rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in drosophila |
| topic | vesicle trafficking Rab5 Rab11 hematopoiesis lymph gland hemocyte |
| url | https://elifesciences.org/articles/60870 |
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