A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen<sup>®</sup> or Taqman<sup>®</sup> Technologies
In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly und...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/4/715 |
| _version_ | 1797536993410809856 |
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| author | Fábio A. Abade dos Santos Carina L. Carvalho Maria C. Peleteiro Francisco Parra Margarida D. Duarte |
| author_facet | Fábio A. Abade dos Santos Carina L. Carvalho Maria C. Peleteiro Francisco Parra Margarida D. Duarte |
| author_sort | Fábio A. Abade dos Santos |
| collection | DOAJ |
| description | In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly understood, since it affects reproduction, it poses an additional threat to the already fragile populations of Iberian hare, demanding prevalence investigations. In this article, we describe the first quantitative molecular method for LeHV-5 detection, using either Taqman or the EvaGreen systems. This method has excellent sensitivity and specificity, it is able to detect 2.1 copies of LeHV-5 DNA and was validated with an internal control targeting the 18S rRNA gene, allowing monitoring extraction and PCR amplification efficiencies. |
| first_indexed | 2024-03-10T12:08:45Z |
| format | Article |
| id | doaj.art-91ee0ca0c4af448d9751077df3d0df39 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T12:08:45Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-91ee0ca0c4af448d9751077df3d0df392023-11-21T16:23:44ZengMDPI AGViruses1999-49152021-04-0113471510.3390/v13040715A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen<sup>®</sup> or Taqman<sup>®</sup> TechnologiesFábio A. Abade dos Santos0Carina L. Carvalho1Maria C. Peleteiro2Francisco Parra3Margarida D. Duarte4Instituto Nacional de Investigação Agrária e Veterinária, Av. da República, Quinta do Marquês, 2780-157 Oeiras, PortugalInstituto Nacional de Investigação Agrária e Veterinária, Av. da República, Quinta do Marquês, 2780-157 Oeiras, PortugalCIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, PortugalInstituto Universitario de Biotecnología de Asturias (IUBA), Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, SpainInstituto Nacional de Investigação Agrária e Veterinária, Av. da República, Quinta do Marquês, 2780-157 Oeiras, PortugalIn late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly understood, since it affects reproduction, it poses an additional threat to the already fragile populations of Iberian hare, demanding prevalence investigations. In this article, we describe the first quantitative molecular method for LeHV-5 detection, using either Taqman or the EvaGreen systems. This method has excellent sensitivity and specificity, it is able to detect 2.1 copies of LeHV-5 DNA and was validated with an internal control targeting the 18S rRNA gene, allowing monitoring extraction and PCR amplification efficiencies.https://www.mdpi.com/1999-4915/13/4/715herpesvirusgammaherpesvirusleporid gammaherpesvirus 5<i>Lepus granatensis</i>Iberian harereproduction |
| spellingShingle | Fábio A. Abade dos Santos Carina L. Carvalho Maria C. Peleteiro Francisco Parra Margarida D. Duarte A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen<sup>®</sup> or Taqman<sup>®</sup> Technologies Viruses herpesvirus gammaherpesvirus leporid gammaherpesvirus 5 <i>Lepus granatensis</i> Iberian hare reproduction |
| title | A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen<sup>®</sup> or Taqman<sup>®</sup> Technologies |
| title_full | A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen<sup>®</sup> or Taqman<sup>®</sup> Technologies |
| title_fullStr | A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen<sup>®</sup> or Taqman<sup>®</sup> Technologies |
| title_full_unstemmed | A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen<sup>®</sup> or Taqman<sup>®</sup> Technologies |
| title_short | A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen<sup>®</sup> or Taqman<sup>®</sup> Technologies |
| title_sort | versatile qpcr for diagnosis of leporid gammaherpesvirus 5 using evagreen sup r sup or taqman sup r sup technologies |
| topic | herpesvirus gammaherpesvirus leporid gammaherpesvirus 5 <i>Lepus granatensis</i> Iberian hare reproduction |
| url | https://www.mdpi.com/1999-4915/13/4/715 |
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