Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides.
Odorants and pheromones are essential to insects as chemical cues for finding food or an appropriate mating partner. These volatile compounds bind to olfactory receptors (Ors) expressed by olfactory sensory neurons. Each insect Or functions as a ligand-gated ion channel and is a heteromeric complex...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3670925?pdf=render |
| _version_ | 1828916681415589888 |
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| author | Tatsuro Nakagawa Kazushige Touhara |
| author_facet | Tatsuro Nakagawa Kazushige Touhara |
| author_sort | Tatsuro Nakagawa |
| collection | DOAJ |
| description | Odorants and pheromones are essential to insects as chemical cues for finding food or an appropriate mating partner. These volatile compounds bind to olfactory receptors (Ors) expressed by olfactory sensory neurons. Each insect Or functions as a ligand-gated ion channel and is a heteromeric complex that comprises one type of canonical Or and a highly conserved Orco subunit. Because there are many Or types, insect Ors can recognize with high specificity a myriad of chemical cues. Cyclic nucleotides can modulate the activity of insect Or-Orco complexes; however, the mechanism of action of these nucleotides is under debate. Here, we show that cyclic nucleotides, including cAMP and cGMP, interact with the silkmoth sex pheromone receptor complex, BmOr-1-BmOrco, from the outside of the cell and that these nucleotides act as antagonists at low concentrations and weak agonists at high concentrations. These cyclic nucleotides do not compete with the sex pheromone, bombykol, for binding to the BmOr-1 subunit. ATP and GTP also weakly inhibited BmOr-1-BmOrco activity, but D-ribose had no effect; these findings indicated that the purine moiety was crucial for the inhibition. Only the bombykol receptors have been so far shown to be subject to modulation by nucleotide-related compounds, indicating that this responsiveness to these compounds is not common for all insect Or-Orco complexes. |
| first_indexed | 2024-12-13T20:38:19Z |
| format | Article |
| id | doaj.art-91f03bdacce645e8bce4c5f04a72bf54 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T20:38:19Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-91f03bdacce645e8bce4c5f04a72bf542022-12-21T23:32:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0186e6377410.1371/journal.pone.0063774Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides.Tatsuro NakagawaKazushige TouharaOdorants and pheromones are essential to insects as chemical cues for finding food or an appropriate mating partner. These volatile compounds bind to olfactory receptors (Ors) expressed by olfactory sensory neurons. Each insect Or functions as a ligand-gated ion channel and is a heteromeric complex that comprises one type of canonical Or and a highly conserved Orco subunit. Because there are many Or types, insect Ors can recognize with high specificity a myriad of chemical cues. Cyclic nucleotides can modulate the activity of insect Or-Orco complexes; however, the mechanism of action of these nucleotides is under debate. Here, we show that cyclic nucleotides, including cAMP and cGMP, interact with the silkmoth sex pheromone receptor complex, BmOr-1-BmOrco, from the outside of the cell and that these nucleotides act as antagonists at low concentrations and weak agonists at high concentrations. These cyclic nucleotides do not compete with the sex pheromone, bombykol, for binding to the BmOr-1 subunit. ATP and GTP also weakly inhibited BmOr-1-BmOrco activity, but D-ribose had no effect; these findings indicated that the purine moiety was crucial for the inhibition. Only the bombykol receptors have been so far shown to be subject to modulation by nucleotide-related compounds, indicating that this responsiveness to these compounds is not common for all insect Or-Orco complexes.http://europepmc.org/articles/PMC3670925?pdf=render |
| spellingShingle | Tatsuro Nakagawa Kazushige Touhara Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides. PLoS ONE |
| title | Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides. |
| title_full | Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides. |
| title_fullStr | Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides. |
| title_full_unstemmed | Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides. |
| title_short | Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides. |
| title_sort | extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides |
| url | http://europepmc.org/articles/PMC3670925?pdf=render |
| work_keys_str_mv | AT tatsuronakagawa extracellularmodulationofthesilkmothsexpheromonereceptoractivitybycyclicnucleotides AT kazushigetouhara extracellularmodulationofthesilkmothsexpheromonereceptoractivitybycyclicnucleotides |