Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves
Senecavirus A (SVA) is a causative agent for vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases of swine including foot-and-mouth disease (FMD). Recently, it was reported that buffalo in Guangdong, China were experiencing clinical symptoms similar to FMD...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/12/2809 |
| _version_ | 1797454915168108544 |
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| author | Alexandra Buckley Lauren Crawford Kyle Hoffman Shollie Falkenberg |
| author_facet | Alexandra Buckley Lauren Crawford Kyle Hoffman Shollie Falkenberg |
| author_sort | Alexandra Buckley |
| collection | DOAJ |
| description | Senecavirus A (SVA) is a causative agent for vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases of swine including foot-and-mouth disease (FMD). Recently, it was reported that buffalo in Guangdong, China were experiencing clinical symptoms similar to FMD including mouth ulcers and lameness tested positive for SVA. The objective of this study was to determine the susceptibility of cattle (<i>Bos taurus</i>) to SVA infection. Initial in vitro work using the PrimeFlow assay demonstrated that bovine cell lines and peripheral blood mononuclear cells from cattle were susceptible to SVA infection. Subsequently, six colostrum-deprived Holstein calves were challenged with SVA intranasally. No vesicular lesions were observed after challenge. Serum, oral, nasal, and rectal swabs tested for SVA nucleic acid did not support significant viral replication and there was no evidence of seroconversion. Therefore, demonstrating cattle from this study were not susceptible to experimental SVA infection. |
| first_indexed | 2024-03-09T15:44:55Z |
| format | Article |
| id | doaj.art-91f1240c99554c0bad6bd27c956abc67 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T15:44:55Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-91f1240c99554c0bad6bd27c956abc672023-11-24T18:40:03ZengMDPI AGViruses1999-49152022-12-011412280910.3390/v14122809Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived CalvesAlexandra Buckley0Lauren Crawford1Kyle Hoffman2Shollie Falkenberg3Virus and Prion Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA 50010, USABacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA 50010, USAVirus and Prion Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA 50010, USARuminant Disease and Immunology Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA 50010, USASenecavirus A (SVA) is a causative agent for vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases of swine including foot-and-mouth disease (FMD). Recently, it was reported that buffalo in Guangdong, China were experiencing clinical symptoms similar to FMD including mouth ulcers and lameness tested positive for SVA. The objective of this study was to determine the susceptibility of cattle (<i>Bos taurus</i>) to SVA infection. Initial in vitro work using the PrimeFlow assay demonstrated that bovine cell lines and peripheral blood mononuclear cells from cattle were susceptible to SVA infection. Subsequently, six colostrum-deprived Holstein calves were challenged with SVA intranasally. No vesicular lesions were observed after challenge. Serum, oral, nasal, and rectal swabs tested for SVA nucleic acid did not support significant viral replication and there was no evidence of seroconversion. Therefore, demonstrating cattle from this study were not susceptible to experimental SVA infection.https://www.mdpi.com/1999-4915/14/12/2809Senecavirus Avesicular diseasecattlesusceptibility |
| spellingShingle | Alexandra Buckley Lauren Crawford Kyle Hoffman Shollie Falkenberg Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves Viruses Senecavirus A vesicular disease cattle susceptibility |
| title | Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves |
| title_full | Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves |
| title_fullStr | Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves |
| title_full_unstemmed | Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves |
| title_short | Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves |
| title_sort | experimental senecavirus a infection of bovine cell lines and colostrum deprived calves |
| topic | Senecavirus A vesicular disease cattle susceptibility |
| url | https://www.mdpi.com/1999-4915/14/12/2809 |
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