Transcriptional Regulator YqeI, Locating at ETT2 Locus, Affects the Pathogenicity of Avian Pathogenic <i>Escherichia coli</i>
Avian pathogenic <i>Escherichia coli</i> (APEC) is the leading cause of systemic infections in poultry worldwide and has a hidden threat to public health. <i>Escherichia coli</i> type three secretion system 2 (ETT2), similar to the <i>Salmonella</i> pathogenicity...
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MDPI AG
2020-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/9/1658 |
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| author | Mei Xue Yating Xiao Dandan Fu Muhammad Akmal Raheem Ying Shao Xiangjun Song Jian Tu Ting Xue Kezong Qi |
| author_facet | Mei Xue Yating Xiao Dandan Fu Muhammad Akmal Raheem Ying Shao Xiangjun Song Jian Tu Ting Xue Kezong Qi |
| author_sort | Mei Xue |
| collection | DOAJ |
| description | Avian pathogenic <i>Escherichia coli</i> (APEC) is the leading cause of systemic infections in poultry worldwide and has a hidden threat to public health. <i>Escherichia coli</i> type three secretion system 2 (ETT2), similar to the <i>Salmonella</i> pathogenicity island SPI1, is widely distributed in APEC and associated with virulence. The function of YqeI, which is one of the hypothetical transcriptional regulators locating at the ETT2 locus of APEC, is unknown. In this study, we successfully obtained the mutant strain AE81Δ<i>yqeI</i> of the wild type strain AE81 and performed the transcriptional profiling assays. Additionally, the transcriptional sequencing results revealed that YqeI influenced localization, locomotion and biological adhesion and so on. The transmission electron microscope observation showed that the wild type strain AE81 possessed long curved flagella, whereas the mutant strain AE81Δ<i>yqeI</i> hardly had any. The strain AE81Δ<i>yqeI</i> exhibited lower motility than AE81 after culturing the dilute bacterial suspension on a semisolid medium. It was also found that the survival ability of AE81Δ<i>yqeI</i> weakened significantly when AE81Δ<i>yqeI</i> was cultured with 0%, 10%, 20%, 30%, 40% and 50% SPF serum in PBS, and AE81Δ<i>yqeI</i> had decreased adherence to DF-1 cells compared with AE81 in the bacterial adhesion assay. The bacterial colonization assay indicated that the virulence of AE81Δ<i>yqeI</i> was reduced in the heart, liver, spleen, and lung. These results confirmed that the transcription regulator YqeI is involved in APEC’s pathogenicity, and this study provides clues for future research. |
| first_indexed | 2024-03-10T16:18:46Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T16:18:46Z |
| publishDate | 2020-09-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-91f191c5541a4498871a37b6edf3d1d22023-11-20T13:51:41ZengMDPI AGAnimals2076-26152020-09-01109165810.3390/ani10091658Transcriptional Regulator YqeI, Locating at ETT2 Locus, Affects the Pathogenicity of Avian Pathogenic <i>Escherichia coli</i>Mei Xue0Yating Xiao1Dandan Fu2Muhammad Akmal Raheem3Ying Shao4Xiangjun Song5Jian Tu6Ting Xue7Kezong Qi8Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei 230036, Anhui, ChinaAvian pathogenic <i>Escherichia coli</i> (APEC) is the leading cause of systemic infections in poultry worldwide and has a hidden threat to public health. <i>Escherichia coli</i> type three secretion system 2 (ETT2), similar to the <i>Salmonella</i> pathogenicity island SPI1, is widely distributed in APEC and associated with virulence. The function of YqeI, which is one of the hypothetical transcriptional regulators locating at the ETT2 locus of APEC, is unknown. In this study, we successfully obtained the mutant strain AE81Δ<i>yqeI</i> of the wild type strain AE81 and performed the transcriptional profiling assays. Additionally, the transcriptional sequencing results revealed that YqeI influenced localization, locomotion and biological adhesion and so on. The transmission electron microscope observation showed that the wild type strain AE81 possessed long curved flagella, whereas the mutant strain AE81Δ<i>yqeI</i> hardly had any. The strain AE81Δ<i>yqeI</i> exhibited lower motility than AE81 after culturing the dilute bacterial suspension on a semisolid medium. It was also found that the survival ability of AE81Δ<i>yqeI</i> weakened significantly when AE81Δ<i>yqeI</i> was cultured with 0%, 10%, 20%, 30%, 40% and 50% SPF serum in PBS, and AE81Δ<i>yqeI</i> had decreased adherence to DF-1 cells compared with AE81 in the bacterial adhesion assay. The bacterial colonization assay indicated that the virulence of AE81Δ<i>yqeI</i> was reduced in the heart, liver, spleen, and lung. These results confirmed that the transcription regulator YqeI is involved in APEC’s pathogenicity, and this study provides clues for future research.https://www.mdpi.com/2076-2615/10/9/1658avian pathogenic <i>Escherichia coli</i>transcription regulatorYqeIpathogenesis |
| spellingShingle | Mei Xue Yating Xiao Dandan Fu Muhammad Akmal Raheem Ying Shao Xiangjun Song Jian Tu Ting Xue Kezong Qi Transcriptional Regulator YqeI, Locating at ETT2 Locus, Affects the Pathogenicity of Avian Pathogenic <i>Escherichia coli</i> Animals avian pathogenic <i>Escherichia coli</i> transcription regulator YqeI pathogenesis |
| title | Transcriptional Regulator YqeI, Locating at ETT2 Locus, Affects the Pathogenicity of Avian Pathogenic <i>Escherichia coli</i> |
| title_full | Transcriptional Regulator YqeI, Locating at ETT2 Locus, Affects the Pathogenicity of Avian Pathogenic <i>Escherichia coli</i> |
| title_fullStr | Transcriptional Regulator YqeI, Locating at ETT2 Locus, Affects the Pathogenicity of Avian Pathogenic <i>Escherichia coli</i> |
| title_full_unstemmed | Transcriptional Regulator YqeI, Locating at ETT2 Locus, Affects the Pathogenicity of Avian Pathogenic <i>Escherichia coli</i> |
| title_short | Transcriptional Regulator YqeI, Locating at ETT2 Locus, Affects the Pathogenicity of Avian Pathogenic <i>Escherichia coli</i> |
| title_sort | transcriptional regulator yqei locating at ett2 locus affects the pathogenicity of avian pathogenic i escherichia coli i |
| topic | avian pathogenic <i>Escherichia coli</i> transcription regulator YqeI pathogenesis |
| url | https://www.mdpi.com/2076-2615/10/9/1658 |
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