Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission
Abstract Background Cryptosporidium is one of the most prevalent parasites infecting both birds and mammals. To examine the prevalence of Cryptosporidium species and evaluate the public health significance of domestic chickens in Guangdong Province, southern China, we analyzed 1001 fecal samples fro...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-022-05267-x |
| _version_ | 1818207253946171392 |
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| author | Xuhui Lin Luyao Xin Meng Qi Minyu Hou Shenquan Liao Nanshan Qi Juan Li Minna Lv Haiming Cai Junjing Hu Jianfei Zhang Xiangbo Ji Mingfei Sun |
| author_facet | Xuhui Lin Luyao Xin Meng Qi Minyu Hou Shenquan Liao Nanshan Qi Juan Li Minna Lv Haiming Cai Junjing Hu Jianfei Zhang Xiangbo Ji Mingfei Sun |
| author_sort | Xuhui Lin |
| collection | DOAJ |
| description | Abstract Background Cryptosporidium is one of the most prevalent parasites infecting both birds and mammals. To examine the prevalence of Cryptosporidium species and evaluate the public health significance of domestic chickens in Guangdong Province, southern China, we analyzed 1001 fecal samples from 43 intensive broiler chicken farms across six distinct geographical regions. Methods Individual DNA samples were subjected to nested PCR-based amplification and sequencing of the small subunit of the nuclear ribosomal RNA gene (SSU rRNA). Analysis of the 60 kDa glycoprotein gene (gp60) was performed to characterize the subtypes of C. meleagridis. Results The overall prevalence of Cryptosporidium was 13.2% (95% CI 11.1–15.3) (24 of 43 farms), with C. meleagridis (7.8%), C. baileyi (4.8%) and mixed infections (0.6%). Using the gp60 gene, three subtype families, IIIb, IIIe and IIIg, were identified, including six subtypes: one novel (IIIgA25G3R1a) and five previously reported (IIIbA23G1R1c, IIIbA24G1R1, IIIbA21G1R1a, IIIeA17G2R1 and IIIeA26G2R1). Within these subtypes, five known subtypes were genetically identical to those identified in humans. Conclusions This is the first report of C. meleagridis in chickens from Guangdong. The frequent occurrence of C. meleagridis in domestic chickens and the common C. meleagridis subtypes identified in both humans and chickens is of public health significance. Our study indicates that broiler chickens represent a potential zoonotic risk for the transmission of Cryptosporidium in this region. Graphical Abstract |
| first_indexed | 2024-12-12T04:25:59Z |
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| id | doaj.art-91f231168cfb40629187b1668040399c |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-12T04:25:59Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-91f231168cfb40629187b1668040399c2022-12-22T00:38:12ZengBMCParasites & Vectors1756-33052022-06-011511810.1186/s13071-022-05267-xDominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmissionXuhui Lin0Luyao Xin1Meng Qi2Minyu Hou3Shenquan Liao4Nanshan Qi5Juan Li6Minna Lv7Haiming Cai8Junjing Hu9Jianfei Zhang10Xiangbo Ji11Mingfei Sun12Zhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesCollege of Animal Science, Tarim UniversityCollege of Animal Science, Tarim UniversityZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesLanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesZhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Institute of Animal Health, Guangdong Academy of Agricultural SciencesAbstract Background Cryptosporidium is one of the most prevalent parasites infecting both birds and mammals. To examine the prevalence of Cryptosporidium species and evaluate the public health significance of domestic chickens in Guangdong Province, southern China, we analyzed 1001 fecal samples from 43 intensive broiler chicken farms across six distinct geographical regions. Methods Individual DNA samples were subjected to nested PCR-based amplification and sequencing of the small subunit of the nuclear ribosomal RNA gene (SSU rRNA). Analysis of the 60 kDa glycoprotein gene (gp60) was performed to characterize the subtypes of C. meleagridis. Results The overall prevalence of Cryptosporidium was 13.2% (95% CI 11.1–15.3) (24 of 43 farms), with C. meleagridis (7.8%), C. baileyi (4.8%) and mixed infections (0.6%). Using the gp60 gene, three subtype families, IIIb, IIIe and IIIg, were identified, including six subtypes: one novel (IIIgA25G3R1a) and five previously reported (IIIbA23G1R1c, IIIbA24G1R1, IIIbA21G1R1a, IIIeA17G2R1 and IIIeA26G2R1). Within these subtypes, five known subtypes were genetically identical to those identified in humans. Conclusions This is the first report of C. meleagridis in chickens from Guangdong. The frequent occurrence of C. meleagridis in domestic chickens and the common C. meleagridis subtypes identified in both humans and chickens is of public health significance. Our study indicates that broiler chickens represent a potential zoonotic risk for the transmission of Cryptosporidium in this region. Graphical Abstracthttps://doi.org/10.1186/s13071-022-05267-xCryptosporidiumSubtypingChickenZoonoticChina |
| spellingShingle | Xuhui Lin Luyao Xin Meng Qi Minyu Hou Shenquan Liao Nanshan Qi Juan Li Minna Lv Haiming Cai Junjing Hu Jianfei Zhang Xiangbo Ji Mingfei Sun Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission Parasites & Vectors Cryptosporidium Subtyping Chicken Zoonotic China |
| title | Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission |
| title_full | Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission |
| title_fullStr | Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission |
| title_full_unstemmed | Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission |
| title_short | Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission |
| title_sort | dominance of the zoonotic pathogen cryptosporidium meleagridis in broiler chickens in guangdong china reveals evidence of cross transmission |
| topic | Cryptosporidium Subtyping Chicken Zoonotic China |
| url | https://doi.org/10.1186/s13071-022-05267-x |
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