Bartonella quintana deploys host and vector temperature-specific transcriptomes.
The bacterial pathogen Bartonella quintana is passed between humans by body lice. B. quintana has adapted to both the human host and body louse vector niches, producing persistent infection with high titer bacterial loads in both the host (up to 10(5) colony-forming units [CFU]/ml) and vector (more...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3595295?pdf=render |
| _version_ | 1818133969224335360 |
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| author | Stephanie Abromaitis Christopher S Nelson Domenic Previte Kyong S Yoon J Marshall Clark Joseph L DeRisi Jane E Koehler |
| author_facet | Stephanie Abromaitis Christopher S Nelson Domenic Previte Kyong S Yoon J Marshall Clark Joseph L DeRisi Jane E Koehler |
| author_sort | Stephanie Abromaitis |
| collection | DOAJ |
| description | The bacterial pathogen Bartonella quintana is passed between humans by body lice. B. quintana has adapted to both the human host and body louse vector niches, producing persistent infection with high titer bacterial loads in both the host (up to 10(5) colony-forming units [CFU]/ml) and vector (more than 10(8) CFU/ml). Using a novel custom microarray platform, we analyzed bacterial transcription at temperatures corresponding to the host (37°C) and vector (28°C), to probe for temperature-specific and growth phase-specific transcriptomes. We observed that transcription of 7% (93 genes) of the B. quintana genome is modified in response to change in growth phase, and that 5% (68 genes) of the genome is temperature-responsive. Among these transcriptional changes in response to temperature shift and growth phase was the induction of known B. quintana virulence genes and several previously unannotated genes. Hemin binding proteins, secretion systems, response regulators, and genes for invasion and cell attachment were prominent among the differentially-regulated B. quintana genes. This study represents the first analysis of global transcriptional responses by B. quintana. In addition, the in vivo experiments provide novel insight into the B. quintana transcriptional program within the body louse environment. These data and approaches will facilitate study of the adaptation mechanisms employed by Bartonella during the transition between human host and arthropod vector. |
| first_indexed | 2024-12-11T09:01:10Z |
| format | Article |
| id | doaj.art-91f51677b1b8490d980fc752a0a4d8c9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T09:01:10Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-91f51677b1b8490d980fc752a0a4d8c92022-12-22T01:13:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5877310.1371/journal.pone.0058773Bartonella quintana deploys host and vector temperature-specific transcriptomes.Stephanie AbromaitisChristopher S NelsonDomenic PreviteKyong S YoonJ Marshall ClarkJoseph L DeRisiJane E KoehlerThe bacterial pathogen Bartonella quintana is passed between humans by body lice. B. quintana has adapted to both the human host and body louse vector niches, producing persistent infection with high titer bacterial loads in both the host (up to 10(5) colony-forming units [CFU]/ml) and vector (more than 10(8) CFU/ml). Using a novel custom microarray platform, we analyzed bacterial transcription at temperatures corresponding to the host (37°C) and vector (28°C), to probe for temperature-specific and growth phase-specific transcriptomes. We observed that transcription of 7% (93 genes) of the B. quintana genome is modified in response to change in growth phase, and that 5% (68 genes) of the genome is temperature-responsive. Among these transcriptional changes in response to temperature shift and growth phase was the induction of known B. quintana virulence genes and several previously unannotated genes. Hemin binding proteins, secretion systems, response regulators, and genes for invasion and cell attachment were prominent among the differentially-regulated B. quintana genes. This study represents the first analysis of global transcriptional responses by B. quintana. In addition, the in vivo experiments provide novel insight into the B. quintana transcriptional program within the body louse environment. These data and approaches will facilitate study of the adaptation mechanisms employed by Bartonella during the transition between human host and arthropod vector.http://europepmc.org/articles/PMC3595295?pdf=render |
| spellingShingle | Stephanie Abromaitis Christopher S Nelson Domenic Previte Kyong S Yoon J Marshall Clark Joseph L DeRisi Jane E Koehler Bartonella quintana deploys host and vector temperature-specific transcriptomes. PLoS ONE |
| title | Bartonella quintana deploys host and vector temperature-specific transcriptomes. |
| title_full | Bartonella quintana deploys host and vector temperature-specific transcriptomes. |
| title_fullStr | Bartonella quintana deploys host and vector temperature-specific transcriptomes. |
| title_full_unstemmed | Bartonella quintana deploys host and vector temperature-specific transcriptomes. |
| title_short | Bartonella quintana deploys host and vector temperature-specific transcriptomes. |
| title_sort | bartonella quintana deploys host and vector temperature specific transcriptomes |
| url | http://europepmc.org/articles/PMC3595295?pdf=render |
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