Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damage
Neural stem/progenitor cell (NSC) transplantation has been shown to effectively improve neurological function in rats with hypoxic-ischemic brain damage. Vascular endothelial growth factor (VEGF) is a signaling protein that stimulates angiogenesis and improves neural regeneration. We hypothesized th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2016;volume=11;issue=9;spage=1456;epage=1463;aulast=Yao |
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| author | Yue Yao Xiang-rong Zheng Shan-shan Zhang Xia Wang Xiao-he Yu Jie-lu Tan Yu-jia Yang |
| author_facet | Yue Yao Xiang-rong Zheng Shan-shan Zhang Xia Wang Xiao-he Yu Jie-lu Tan Yu-jia Yang |
| author_sort | Yue Yao |
| collection | DOAJ |
| description | Neural stem/progenitor cell (NSC) transplantation has been shown to effectively improve neurological function in rats with hypoxic-ischemic brain damage. Vascular endothelial growth factor (VEGF) is a signaling protein that stimulates angiogenesis and improves neural regeneration. We hypothesized that transplantation of VEGF-transfected NSCs would alleviate hypoxic-ischemic brain damage in neonatal rats. We produced and transfected a recombinant lentiviral vector containing the VEGF165 gene into cultured NSCs. The transfected NSCs were transplanted into the left sensorimotor cortex of rats 3 days after hypoxic-ischemic brain damage. Compared with the NSCs group, VEGF mRNA and protein expression levels were increased in the transgene NSCs group, and learning and memory abilities were significantly improved at 30 days. Furthermore, histopathological changes were alleviated in these animals. Our findings indicate that transplantation of VEGF-transfected NSCs may facilitate the recovery of neurological function, and that its therapeutic effectiveness is better than that of unmodified NSCs. |
| first_indexed | 2024-04-12T08:09:00Z |
| format | Article |
| id | doaj.art-91f83760635e44f985c52afd8c5d99e1 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-12T08:09:00Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-91f83760635e44f985c52afd8c5d99e12022-12-22T03:41:03ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742016-01-011191456146310.4103/1673-5374.191220Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damageYue YaoXiang-rong ZhengShan-shan ZhangXia WangXiao-he YuJie-lu TanYu-jia YangNeural stem/progenitor cell (NSC) transplantation has been shown to effectively improve neurological function in rats with hypoxic-ischemic brain damage. Vascular endothelial growth factor (VEGF) is a signaling protein that stimulates angiogenesis and improves neural regeneration. We hypothesized that transplantation of VEGF-transfected NSCs would alleviate hypoxic-ischemic brain damage in neonatal rats. We produced and transfected a recombinant lentiviral vector containing the VEGF165 gene into cultured NSCs. The transfected NSCs were transplanted into the left sensorimotor cortex of rats 3 days after hypoxic-ischemic brain damage. Compared with the NSCs group, VEGF mRNA and protein expression levels were increased in the transgene NSCs group, and learning and memory abilities were significantly improved at 30 days. Furthermore, histopathological changes were alleviated in these animals. Our findings indicate that transplantation of VEGF-transfected NSCs may facilitate the recovery of neurological function, and that its therapeutic effectiveness is better than that of unmodified NSCs.http://www.nrronline.org/article.asp?issn=1673-5374;year=2016;volume=11;issue=9;spage=1456;epage=1463;aulast=Yaonerve regeneration; vascular endothelial growth factor; transfection; neural stem/progenitor cells; transplantation; hypoxic-ischemic brain damage; cerebral cortex; animal model; neuroprotection; neural regeneration |
| spellingShingle | Yue Yao Xiang-rong Zheng Shan-shan Zhang Xia Wang Xiao-he Yu Jie-lu Tan Yu-jia Yang Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damage Neural Regeneration Research nerve regeneration; vascular endothelial growth factor; transfection; neural stem/progenitor cells; transplantation; hypoxic-ischemic brain damage; cerebral cortex; animal model; neuroprotection; neural regeneration |
| title | Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damage |
| title_full | Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damage |
| title_fullStr | Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damage |
| title_full_unstemmed | Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damage |
| title_short | Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damage |
| title_sort | transplantation of vascular endothelial growth factor modified neural stem progenitor cells promotes the recovery of neurological function following hypoxic ischemic brain damage |
| topic | nerve regeneration; vascular endothelial growth factor; transfection; neural stem/progenitor cells; transplantation; hypoxic-ischemic brain damage; cerebral cortex; animal model; neuroprotection; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2016;volume=11;issue=9;spage=1456;epage=1463;aulast=Yao |
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