Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering
Bone marrow-derived mesenchymal stem cells (MSCs) offer a promising therapeutic method for cardiac tissue regeneration. However, to monitor the fate of MSCs for tissue repair, a better stem cell delivery carrier is needed. Developing a unique injectable and shear-thinning dual cross-linked hybrid hy...
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MDPI AG
2022-02-01
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author | Samaneh Soltani Rahmatollah Emadi Shaghayegh Haghjooy Javanmard Mahshid Kharaziha Abbas Rahmati Vijay Kumar Thakur Saeid Lotfian |
author_facet | Samaneh Soltani Rahmatollah Emadi Shaghayegh Haghjooy Javanmard Mahshid Kharaziha Abbas Rahmati Vijay Kumar Thakur Saeid Lotfian |
author_sort | Samaneh Soltani |
collection | DOAJ |
description | Bone marrow-derived mesenchymal stem cells (MSCs) offer a promising therapeutic method for cardiac tissue regeneration. However, to monitor the fate of MSCs for tissue repair, a better stem cell delivery carrier is needed. Developing a unique injectable and shear-thinning dual cross-linked hybrid hydrogel for MSC delivery for cardiac tissue engineering is highly desirable. This hydrogel was synthesised using guest: host reaction based on alginate-cyclodextrin (Alg-CD) and adamantane-graphene oxide (Ad-GO). Here, the role of macromere concentration (10 and 12%) on the MSC function is discussed. Our hybrid hydrogels reveal a suitable oxygen pathway required for cell survival. However, this value is strongly dependent on the macromere concentrations, while the hydrogels with 12% macromere concentration (2DC12) significantly enhanced the oxygen permeability value (1.16-fold). Moreover, after two weeks of culture, rat MSCs (rMSCs) encapsulated in Alg-GO hydrogels expressed troponin T (TNT) and GATA4 markers. Noticeably, the 2DC12 hydrogels enhance rMSCs differentiation markers (1.30-times for TNT and 1.21-times for GATA4). Overall, our findings indicate that tuning the hydrogel compositions regulates the fate of encapsulated rMSCs within hydrogels. These outcomes may promote the advancement of new multifunctional platforms that consider the spatial and transient guidelines of undifferentiated cell destiny and capacity even after transplantation for heart tissue regeneration. |
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issn | 2310-2861 |
language | English |
last_indexed | 2024-03-09T21:53:55Z |
publishDate | 2022-02-01 |
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series | Gels |
spelling | doaj.art-91f86f5643fe452da68fc017711cd2d02023-11-23T20:02:08ZengMDPI AGGels2310-28612022-02-018212110.3390/gels8020121Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue EngineeringSamaneh Soltani0Rahmatollah Emadi1Shaghayegh Haghjooy Javanmard2Mahshid Kharaziha3Abbas Rahmati4Vijay Kumar Thakur5Saeid Lotfian6Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, IranBiomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, IranApplied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81746-73461, IranBiomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, IranDepartment of Chemistry, University of Isfahan, Isfahan 81746-73441, IranBiorefining and Advanced Materials Research Center, Scotland’s Rural College (SRUC), Kings Buildings, Edinburgh EH9 3JG, UKFaculty of Engineering, University of Strathclyde, Glasgow G4 0LZ, UKBone marrow-derived mesenchymal stem cells (MSCs) offer a promising therapeutic method for cardiac tissue regeneration. However, to monitor the fate of MSCs for tissue repair, a better stem cell delivery carrier is needed. Developing a unique injectable and shear-thinning dual cross-linked hybrid hydrogel for MSC delivery for cardiac tissue engineering is highly desirable. This hydrogel was synthesised using guest: host reaction based on alginate-cyclodextrin (Alg-CD) and adamantane-graphene oxide (Ad-GO). Here, the role of macromere concentration (10 and 12%) on the MSC function is discussed. Our hybrid hydrogels reveal a suitable oxygen pathway required for cell survival. However, this value is strongly dependent on the macromere concentrations, while the hydrogels with 12% macromere concentration (2DC12) significantly enhanced the oxygen permeability value (1.16-fold). Moreover, after two weeks of culture, rat MSCs (rMSCs) encapsulated in Alg-GO hydrogels expressed troponin T (TNT) and GATA4 markers. Noticeably, the 2DC12 hydrogels enhance rMSCs differentiation markers (1.30-times for TNT and 1.21-times for GATA4). Overall, our findings indicate that tuning the hydrogel compositions regulates the fate of encapsulated rMSCs within hydrogels. These outcomes may promote the advancement of new multifunctional platforms that consider the spatial and transient guidelines of undifferentiated cell destiny and capacity even after transplantation for heart tissue regeneration.https://www.mdpi.com/2310-2861/8/2/121shear-thinningdual-cross linked hydrogelsmesenchymal stem cellscardiac tissue engineering |
spellingShingle | Samaneh Soltani Rahmatollah Emadi Shaghayegh Haghjooy Javanmard Mahshid Kharaziha Abbas Rahmati Vijay Kumar Thakur Saeid Lotfian Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering Gels shear-thinning dual-cross linked hydrogels mesenchymal stem cells cardiac tissue engineering |
title | Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering |
title_full | Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering |
title_fullStr | Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering |
title_full_unstemmed | Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering |
title_short | Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering |
title_sort | development of an injectable shear thinning nanocomposite hydrogel for cardiac tissue engineering |
topic | shear-thinning dual-cross linked hydrogels mesenchymal stem cells cardiac tissue engineering |
url | https://www.mdpi.com/2310-2861/8/2/121 |
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