Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering

Bone marrow-derived mesenchymal stem cells (MSCs) offer a promising therapeutic method for cardiac tissue regeneration. However, to monitor the fate of MSCs for tissue repair, a better stem cell delivery carrier is needed. Developing a unique injectable and shear-thinning dual cross-linked hybrid hy...

Full description

Bibliographic Details
Main Authors: Samaneh Soltani, Rahmatollah Emadi, Shaghayegh Haghjooy Javanmard, Mahshid Kharaziha, Abbas Rahmati, Vijay Kumar Thakur, Saeid Lotfian
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Gels
Subjects:
Online Access:https://www.mdpi.com/2310-2861/8/2/121
_version_ 1797480006696304640
author Samaneh Soltani
Rahmatollah Emadi
Shaghayegh Haghjooy Javanmard
Mahshid Kharaziha
Abbas Rahmati
Vijay Kumar Thakur
Saeid Lotfian
author_facet Samaneh Soltani
Rahmatollah Emadi
Shaghayegh Haghjooy Javanmard
Mahshid Kharaziha
Abbas Rahmati
Vijay Kumar Thakur
Saeid Lotfian
author_sort Samaneh Soltani
collection DOAJ
description Bone marrow-derived mesenchymal stem cells (MSCs) offer a promising therapeutic method for cardiac tissue regeneration. However, to monitor the fate of MSCs for tissue repair, a better stem cell delivery carrier is needed. Developing a unique injectable and shear-thinning dual cross-linked hybrid hydrogel for MSC delivery for cardiac tissue engineering is highly desirable. This hydrogel was synthesised using guest: host reaction based on alginate-cyclodextrin (Alg-CD) and adamantane-graphene oxide (Ad-GO). Here, the role of macromere concentration (10 and 12%) on the MSC function is discussed. Our hybrid hydrogels reveal a suitable oxygen pathway required for cell survival. However, this value is strongly dependent on the macromere concentrations, while the hydrogels with 12% macromere concentration (2DC12) significantly enhanced the oxygen permeability value (1.16-fold). Moreover, after two weeks of culture, rat MSCs (rMSCs) encapsulated in Alg-GO hydrogels expressed troponin T (TNT) and GATA4 markers. Noticeably, the 2DC12 hydrogels enhance rMSCs differentiation markers (1.30-times for TNT and 1.21-times for GATA4). Overall, our findings indicate that tuning the hydrogel compositions regulates the fate of encapsulated rMSCs within hydrogels. These outcomes may promote the advancement of new multifunctional platforms that consider the spatial and transient guidelines of undifferentiated cell destiny and capacity even after transplantation for heart tissue regeneration.
first_indexed 2024-03-09T21:53:55Z
format Article
id doaj.art-91f86f5643fe452da68fc017711cd2d0
institution Directory Open Access Journal
issn 2310-2861
language English
last_indexed 2024-03-09T21:53:55Z
publishDate 2022-02-01
publisher MDPI AG
record_format Article
series Gels
spelling doaj.art-91f86f5643fe452da68fc017711cd2d02023-11-23T20:02:08ZengMDPI AGGels2310-28612022-02-018212110.3390/gels8020121Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue EngineeringSamaneh Soltani0Rahmatollah Emadi1Shaghayegh Haghjooy Javanmard2Mahshid Kharaziha3Abbas Rahmati4Vijay Kumar Thakur5Saeid Lotfian6Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, IranBiomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, IranApplied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81746-73461, IranBiomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, IranDepartment of Chemistry, University of Isfahan, Isfahan 81746-73441, IranBiorefining and Advanced Materials Research Center, Scotland’s Rural College (SRUC), Kings Buildings, Edinburgh EH9 3JG, UKFaculty of Engineering, University of Strathclyde, Glasgow G4 0LZ, UKBone marrow-derived mesenchymal stem cells (MSCs) offer a promising therapeutic method for cardiac tissue regeneration. However, to monitor the fate of MSCs for tissue repair, a better stem cell delivery carrier is needed. Developing a unique injectable and shear-thinning dual cross-linked hybrid hydrogel for MSC delivery for cardiac tissue engineering is highly desirable. This hydrogel was synthesised using guest: host reaction based on alginate-cyclodextrin (Alg-CD) and adamantane-graphene oxide (Ad-GO). Here, the role of macromere concentration (10 and 12%) on the MSC function is discussed. Our hybrid hydrogels reveal a suitable oxygen pathway required for cell survival. However, this value is strongly dependent on the macromere concentrations, while the hydrogels with 12% macromere concentration (2DC12) significantly enhanced the oxygen permeability value (1.16-fold). Moreover, after two weeks of culture, rat MSCs (rMSCs) encapsulated in Alg-GO hydrogels expressed troponin T (TNT) and GATA4 markers. Noticeably, the 2DC12 hydrogels enhance rMSCs differentiation markers (1.30-times for TNT and 1.21-times for GATA4). Overall, our findings indicate that tuning the hydrogel compositions regulates the fate of encapsulated rMSCs within hydrogels. These outcomes may promote the advancement of new multifunctional platforms that consider the spatial and transient guidelines of undifferentiated cell destiny and capacity even after transplantation for heart tissue regeneration.https://www.mdpi.com/2310-2861/8/2/121shear-thinningdual-cross linked hydrogelsmesenchymal stem cellscardiac tissue engineering
spellingShingle Samaneh Soltani
Rahmatollah Emadi
Shaghayegh Haghjooy Javanmard
Mahshid Kharaziha
Abbas Rahmati
Vijay Kumar Thakur
Saeid Lotfian
Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering
Gels
shear-thinning
dual-cross linked hydrogels
mesenchymal stem cells
cardiac tissue engineering
title Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering
title_full Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering
title_fullStr Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering
title_full_unstemmed Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering
title_short Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering
title_sort development of an injectable shear thinning nanocomposite hydrogel for cardiac tissue engineering
topic shear-thinning
dual-cross linked hydrogels
mesenchymal stem cells
cardiac tissue engineering
url https://www.mdpi.com/2310-2861/8/2/121
work_keys_str_mv AT samanehsoltani developmentofaninjectableshearthinningnanocompositehydrogelforcardiactissueengineering
AT rahmatollahemadi developmentofaninjectableshearthinningnanocompositehydrogelforcardiactissueengineering
AT shaghayeghhaghjooyjavanmard developmentofaninjectableshearthinningnanocompositehydrogelforcardiactissueengineering
AT mahshidkharaziha developmentofaninjectableshearthinningnanocompositehydrogelforcardiactissueengineering
AT abbasrahmati developmentofaninjectableshearthinningnanocompositehydrogelforcardiactissueengineering
AT vijaykumarthakur developmentofaninjectableshearthinningnanocompositehydrogelforcardiactissueengineering
AT saeidlotfian developmentofaninjectableshearthinningnanocompositehydrogelforcardiactissueengineering