A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection
In this study, a label-free and antibody-free impedimetric biosensor based on molecularly imprinting technology for exosomes derived from non-small-cell lung cancer (NSCLC) cells was established. Involved preparation parameters were systematically investigated. In this design, with template exosomes...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/13/6/647 |
| _version_ | 1827738263240048640 |
|---|---|
| author | Jingbo Zhang Quancheng Chen Xuemin Gao Qian Lin Ziqin Suo Di Wu Xijie Wu Qing Chen |
| author_facet | Jingbo Zhang Quancheng Chen Xuemin Gao Qian Lin Ziqin Suo Di Wu Xijie Wu Qing Chen |
| author_sort | Jingbo Zhang |
| collection | DOAJ |
| description | In this study, a label-free and antibody-free impedimetric biosensor based on molecularly imprinting technology for exosomes derived from non-small-cell lung cancer (NSCLC) cells was established. Involved preparation parameters were systematically investigated. In this design, with template exosomes anchored on a glassy carbon electrode (GCE) by decorated cholesterol molecules, the subsequent electro-polymerization of APBA and elution procedure afforded a selective adsorption membrane for template A549 exosomes. The adsorption of exosomes caused a rise in the impedance of the sensor, so the concentration of template exosomes can be quantified by monitoring the impedance of GCEs. Each procedure in the establishment of the sensor was monitored with a corresponding method. Methodological verification showed great sensitivity and selectivity of this method with an LOD = 2.03 × 10<sup>3</sup> and an LOQ = 4.10 × 10<sup>4</sup> particles/mL. By introducing normal cells and other cancer cells derived exosomes as interference, high selectivity was proved. Accuracy and precision were measured, with an obtained average recovery ratio of 100.76% and a resulting RSD of 1.86%. Additionally, the sensors’ performance was retained at 4 °C for a week or after undergoing elution and re-adsorption cycles seven times. In summary, the sensor is competitive for clinical translational application and improving the prognosis and survival for NSCLC patients. |
| first_indexed | 2024-03-11T02:41:57Z |
| format | Article |
| id | doaj.art-91fac492bf384414adc156b66084fdb9 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T02:41:57Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-91fac492bf384414adc156b66084fdb92023-11-18T09:33:10ZengMDPI AGBiosensors2079-63742023-06-0113664710.3390/bios13060647A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes DetectionJingbo Zhang0Quancheng Chen1Xuemin Gao2Qian Lin3Ziqin Suo4Di Wu5Xijie Wu6Qing Chen7School of Pharmaceutical Science, Xiamen University, 4221 Xiang’an South Road, Xiamen 361102, ChinaSchool of Pharmaceutical Science, Xiamen University, 4221 Xiang’an South Road, Xiamen 361102, ChinaSchool of Pharmaceutical Science, Xiamen University, 4221 Xiang’an South Road, Xiamen 361102, ChinaSchool of Pharmaceutical Science, Xiamen University, 4221 Xiang’an South Road, Xiamen 361102, ChinaSchool of Pharmaceutical Science, Xiamen University, 4221 Xiang’an South Road, Xiamen 361102, ChinaSchool of Pharmaceutical Science, Xiamen University, 4221 Xiang’an South Road, Xiamen 361102, ChinaDepartment of Cardiac Surgery, Xiamen Cardiovascular Hospital, Xiamen University, 2999 Jinshan Road, Xiamen 361010, ChinaSchool of Pharmaceutical Science, Xiamen University, 4221 Xiang’an South Road, Xiamen 361102, ChinaIn this study, a label-free and antibody-free impedimetric biosensor based on molecularly imprinting technology for exosomes derived from non-small-cell lung cancer (NSCLC) cells was established. Involved preparation parameters were systematically investigated. In this design, with template exosomes anchored on a glassy carbon electrode (GCE) by decorated cholesterol molecules, the subsequent electro-polymerization of APBA and elution procedure afforded a selective adsorption membrane for template A549 exosomes. The adsorption of exosomes caused a rise in the impedance of the sensor, so the concentration of template exosomes can be quantified by monitoring the impedance of GCEs. Each procedure in the establishment of the sensor was monitored with a corresponding method. Methodological verification showed great sensitivity and selectivity of this method with an LOD = 2.03 × 10<sup>3</sup> and an LOQ = 4.10 × 10<sup>4</sup> particles/mL. By introducing normal cells and other cancer cells derived exosomes as interference, high selectivity was proved. Accuracy and precision were measured, with an obtained average recovery ratio of 100.76% and a resulting RSD of 1.86%. Additionally, the sensors’ performance was retained at 4 °C for a week or after undergoing elution and re-adsorption cycles seven times. In summary, the sensor is competitive for clinical translational application and improving the prognosis and survival for NSCLC patients.https://www.mdpi.com/2079-6374/13/6/647exosomesmolecular imprinted polymers (MIPs)antibody-freeimpedimetric sensor |
| spellingShingle | Jingbo Zhang Quancheng Chen Xuemin Gao Qian Lin Ziqin Suo Di Wu Xijie Wu Qing Chen A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection Biosensors exosomes molecular imprinted polymers (MIPs) antibody-free impedimetric sensor |
| title | A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection |
| title_full | A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection |
| title_fullStr | A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection |
| title_full_unstemmed | A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection |
| title_short | A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection |
| title_sort | label free and antibody free molecularly imprinted polymer based impedimetric sensor for nsclc cells derived exosomes detection |
| topic | exosomes molecular imprinted polymers (MIPs) antibody-free impedimetric sensor |
| url | https://www.mdpi.com/2079-6374/13/6/647 |
| work_keys_str_mv | AT jingbozhang alabelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT quanchengchen alabelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT xuemingao alabelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT qianlin alabelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT ziqinsuo alabelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT diwu alabelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT xijiewu alabelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT qingchen alabelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT jingbozhang labelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT quanchengchen labelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT xuemingao labelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT qianlin labelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT ziqinsuo labelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT diwu labelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT xijiewu labelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection AT qingchen labelfreeandantibodyfreemolecularlyimprintedpolymerbasedimpedimetricsensorfornsclccellsderivedexosomesdetection |