Expression of VEGF and CD34 in Psoriasis Vulgaris: Correlation with Histological Grading by Trozak Histological Assessment Score
Introduction: Psoriasis is a chronic inflammatory immunemediated cutaneous disorder. Histologically, it shows epidermal proliferation and neovascularisation in papillary dermis. Histological grading of psoriasis is done using Trozak Histological assessment Score (THS). Angiogenesis drives psorias...
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JCDR Research and Publications Pvt. Ltd.
2022-07-01
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| Series: | National Journal of Laboratory Medicine |
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| author | R SUJATHA MANGAL V KULKARNI RUFAIDA YA MANJUNATHA4 |
| author_facet | R SUJATHA MANGAL V KULKARNI RUFAIDA YA MANJUNATHA4 |
| author_sort | R SUJATHA |
| collection | DOAJ |
| description | Introduction: Psoriasis is a chronic inflammatory immunemediated cutaneous disorder. Histologically, it shows epidermal
proliferation and neovascularisation in papillary dermis.
Histological grading of psoriasis is done using Trozak Histological
assessment Score (THS). Angiogenesis drives psoriasis. Vascular
Endothelial Growth Factor (VEGF) and Cluster of Differentiation
(CD34)/Micro Vessel Density (MVD) are proangiogenic cytokines
in psoriasis and are overexpressed in psoriatic skin when
compared to normal controls.
Aim: 1) To analyse and compare the IHC expression of VEGF and
CD34/MVD in skin biopsies of psoriasis cases and controls; 2) To
compare the expression of VEGF, CD34/MVD with histological
grade done by THS.
Materials and Methods: A retrospective study was done in the
Department of Pathology at Dr. B.R. Ambedkar Medical College and
Hospital, Bengaluru, Karnataka, India, between September 2020 to
August 2021. Twenty five cases and 25 controls (normal skin) were
included in the present study. Histopathology/Immunohistochemistry
(IHC) for VEGF and CD34 was performed. Suprabasilar layers of
the epidermis were assessed for VEGF expression. Cell showing
granular positivity within the cytoplasm was considered positive for
VEGF. The mean percentage of positive cells was determined in
a minimum of five areas at X400 magnification. CD34/MVD was
calculated as the number of vessels in three high density locations.
The MVD/High Power Field (HPF) for each individual lesion and
graded as <4: negative 1+:4-10 capillaries, 2+:11-20, 3+: >21 per
HPF. The THS was done as per standard protocol.
Results: The age range in the present study was 18-68 years
with male:female ratio of 1.2:1. An increased VEGF expression
(p-value<0.001) in epidermal keratinocytes with accentuated
MVD (p-value <0.001) in papillary dermis was seen in all 25
cases which was higher when compared to normal skin. There
was positive correlation between VEGF and CD34 (Spearman's
rank correlation=0.886). The mean THS was 9.56±3.4 and had
positive correlation with VEGF and MVD (r=0.809 and 0.867,
respectively) and was statistically significant.
Conclusion: The present study shows that VEGF and CD34 are
overexpressed in psoriasis utility for assessing disease severity
and further provide insights for targeted therapy against VEGF
pathway. Histological grading of psoriasis can give a baseline to
evaluate response to treatment. |
| first_indexed | 2024-04-13T06:54:13Z |
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| language | English |
| last_indexed | 2024-04-13T06:54:13Z |
| publishDate | 2022-07-01 |
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| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-9202416dd88a45ebb60e4496561f6c402022-12-22T02:57:18ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822022-07-01113586110.7860/NJLM/2022/53492.2649Expression of VEGF and CD34 in Psoriasis Vulgaris: Correlation with Histological Grading by Trozak Histological Assessment ScoreR SUJATHA0MANGAL V KULKARNI1RUFAIDA2YA MANJUNATHA43Associate Professor, Department of Pathology, Dr. B.R. Ambedkar Medical College and Hospital, Bengaluru, Karnataka, India. Postgraduate, Department of Pathology, Dr. B.R. Ambedkar Medical College and Hospital, Bengaluru, Karnataka, IndiaPostgraduate, Department of Pathology, Dr. B.R. Ambedkar Medical College and Hospital, Bengaluru, Karnataka, India.Professor and Head, Department of Pathology, Dr. B.R. Ambedkar Medical College and Hospital, Bengaluru, Karnataka, India.Introduction: Psoriasis is a chronic inflammatory immunemediated cutaneous disorder. Histologically, it shows epidermal proliferation and neovascularisation in papillary dermis. Histological grading of psoriasis is done using Trozak Histological assessment Score (THS). Angiogenesis drives psoriasis. Vascular Endothelial Growth Factor (VEGF) and Cluster of Differentiation (CD34)/Micro Vessel Density (MVD) are proangiogenic cytokines in psoriasis and are overexpressed in psoriatic skin when compared to normal controls. Aim: 1) To analyse and compare the IHC expression of VEGF and CD34/MVD in skin biopsies of psoriasis cases and controls; 2) To compare the expression of VEGF, CD34/MVD with histological grade done by THS. Materials and Methods: A retrospective study was done in the Department of Pathology at Dr. B.R. Ambedkar Medical College and Hospital, Bengaluru, Karnataka, India, between September 2020 to August 2021. Twenty five cases and 25 controls (normal skin) were included in the present study. Histopathology/Immunohistochemistry (IHC) for VEGF and CD34 was performed. Suprabasilar layers of the epidermis were assessed for VEGF expression. Cell showing granular positivity within the cytoplasm was considered positive for VEGF. The mean percentage of positive cells was determined in a minimum of five areas at X400 magnification. CD34/MVD was calculated as the number of vessels in three high density locations. The MVD/High Power Field (HPF) for each individual lesion and graded as <4: negative 1+:4-10 capillaries, 2+:11-20, 3+: >21 per HPF. The THS was done as per standard protocol. Results: The age range in the present study was 18-68 years with male:female ratio of 1.2:1. An increased VEGF expression (p-value<0.001) in epidermal keratinocytes with accentuated MVD (p-value <0.001) in papillary dermis was seen in all 25 cases which was higher when compared to normal skin. There was positive correlation between VEGF and CD34 (Spearman's rank correlation=0.886). The mean THS was 9.56±3.4 and had positive correlation with VEGF and MVD (r=0.809 and 0.867, respectively) and was statistically significant. Conclusion: The present study shows that VEGF and CD34 are overexpressed in psoriasis utility for assessing disease severity and further provide insights for targeted therapy against VEGF pathway. Histological grading of psoriasis can give a baseline to evaluate response to treatment.https://www.njlm.net/articles/PDF/2649/53492_CE[Ra1]_F[SH]_PF1(AG_SHU)_PFA(SHU)_PB(AG_SHU)_PN(SHU).pdfhigh power fieldimmunohistochemistrymicro vessel densityvascular endothelial growth factor |
| spellingShingle | R SUJATHA MANGAL V KULKARNI RUFAIDA YA MANJUNATHA4 Expression of VEGF and CD34 in Psoriasis Vulgaris: Correlation with Histological Grading by Trozak Histological Assessment Score National Journal of Laboratory Medicine high power field immunohistochemistry micro vessel density vascular endothelial growth factor |
| title | Expression of VEGF and CD34 in Psoriasis Vulgaris: Correlation with Histological Grading by Trozak Histological Assessment Score |
| title_full | Expression of VEGF and CD34 in Psoriasis Vulgaris: Correlation with Histological Grading by Trozak Histological Assessment Score |
| title_fullStr | Expression of VEGF and CD34 in Psoriasis Vulgaris: Correlation with Histological Grading by Trozak Histological Assessment Score |
| title_full_unstemmed | Expression of VEGF and CD34 in Psoriasis Vulgaris: Correlation with Histological Grading by Trozak Histological Assessment Score |
| title_short | Expression of VEGF and CD34 in Psoriasis Vulgaris: Correlation with Histological Grading by Trozak Histological Assessment Score |
| title_sort | expression of vegf and cd34 in psoriasis vulgaris correlation with histological grading by trozak histological assessment score |
| topic | high power field immunohistochemistry micro vessel density vascular endothelial growth factor |
| url | https://www.njlm.net/articles/PDF/2649/53492_CE[Ra1]_F[SH]_PF1(AG_SHU)_PFA(SHU)_PB(AG_SHU)_PN(SHU).pdf |
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