Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysis
Individuals fail to elicit protective antibody after hepatitis B vaccination remain at risk for hepatitis B virus infection. Analysis of the transcriptome of peripheral blood mononuclear cells (PBMCs) is essential to elucidate the characteristics of gene expression in non-responders. In this study,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-07-01
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| Series: | Human Vaccines & Immunotherapeutics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21645515.2018.1450122 |
| _version_ | 1797677487158722560 |
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| author | Shaohui Qiu Peng He Xin Fang Haiqing Tong Jingjing Lv Jiaye Liu Li Zhang Xiangjun Zhai Liying Wang Zhongyu Hu Yongli Yu |
| author_facet | Shaohui Qiu Peng He Xin Fang Haiqing Tong Jingjing Lv Jiaye Liu Li Zhang Xiangjun Zhai Liying Wang Zhongyu Hu Yongli Yu |
| author_sort | Shaohui Qiu |
| collection | DOAJ |
| description | Individuals fail to elicit protective antibody after hepatitis B vaccination remain at risk for hepatitis B virus infection. Analysis of the transcriptome of peripheral blood mononuclear cells (PBMCs) is essential to elucidate the characteristics of gene expression in non-responders. In this study, we enrolled seven responders who had received three injections and seven non-responders who had six injections of hepatitis B vaccine before. All the participants were then vaccinated with a three-dose boost regimen. Microarray analysis and Luminex assay were applied to examine mRNA expression and Th1/Th2/Th9/Th17/Th22/Treg cytokine and chemokine profiles in non-responders and responders. Differentially expressed genes in PBMCs of non-responders at 5 time points, i.e. pre-vaccination, 3rd, 7th, 28th day post the first dose vaccination and 7th day post the second dose vaccination indicated a dense network trend. Compared with responders, nine coding genes (BPI, DEFA1B, DEFA4, CEACAM8, MMP8, FOLR3, LTF, TCN1 and TKTL1) were significantly up-regulated in non-responders at all 5 time points, which could probably be the characteristic genes in hepatitis B vaccine non-responsiveness. Gene ontology analysis revealed that most of the DEGs were related with immune responses. Validation results of these 9 genes using quantitative real-time polymerase chain reaction were mostly consistent with the results of microarray. Cytokine analysis demonstrated that IL-27 and CXCL12 concentrations in responders were significantly higher than non-responders on the 3rd day after the first dose and 7th day after the second dose of vaccination, respectively. No significant difference was observed in other cytokine and chemokine signatures between the two groups. In conclusion, our results revealed characteristic transcriptome and cytokine changes in hepatitis B vaccine non-responders after boost immunization. |
| first_indexed | 2024-03-11T22:45:51Z |
| format | Article |
| id | doaj.art-9203a40fb44a47a48e8754cdaaa98333 |
| institution | Directory Open Access Journal |
| issn | 2164-5515 2164-554X |
| language | English |
| last_indexed | 2024-03-11T22:45:51Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj.art-9203a40fb44a47a48e8754cdaaa983332023-09-22T08:17:55ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2018-07-011471763177210.1080/21645515.2018.14501221450122Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysisShaohui Qiu0Peng He1Xin Fang2Haiqing Tong3Jingjing Lv4Jiaye Liu5Li Zhang6Xiangjun Zhai7Liying Wang8Zhongyu Hu9Yongli Yu10College of Basic Medical Sciences, Jilin UniversityKey Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug ControlKey Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug ControlKey Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug ControlShandong Provincial Center for Disease Control and PreventionShandong Provincial Center for Disease Control and PreventionShandong Provincial Center for Disease Control and PreventionJiangsu Provincial Center for Disease Control and PreventionCollege of Basic Medical Sciences, Jilin UniversityKey Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug ControlCollege of Basic Medical Sciences, Jilin UniversityIndividuals fail to elicit protective antibody after hepatitis B vaccination remain at risk for hepatitis B virus infection. Analysis of the transcriptome of peripheral blood mononuclear cells (PBMCs) is essential to elucidate the characteristics of gene expression in non-responders. In this study, we enrolled seven responders who had received three injections and seven non-responders who had six injections of hepatitis B vaccine before. All the participants were then vaccinated with a three-dose boost regimen. Microarray analysis and Luminex assay were applied to examine mRNA expression and Th1/Th2/Th9/Th17/Th22/Treg cytokine and chemokine profiles in non-responders and responders. Differentially expressed genes in PBMCs of non-responders at 5 time points, i.e. pre-vaccination, 3rd, 7th, 28th day post the first dose vaccination and 7th day post the second dose vaccination indicated a dense network trend. Compared with responders, nine coding genes (BPI, DEFA1B, DEFA4, CEACAM8, MMP8, FOLR3, LTF, TCN1 and TKTL1) were significantly up-regulated in non-responders at all 5 time points, which could probably be the characteristic genes in hepatitis B vaccine non-responsiveness. Gene ontology analysis revealed that most of the DEGs were related with immune responses. Validation results of these 9 genes using quantitative real-time polymerase chain reaction were mostly consistent with the results of microarray. Cytokine analysis demonstrated that IL-27 and CXCL12 concentrations in responders were significantly higher than non-responders on the 3rd day after the first dose and 7th day after the second dose of vaccination, respectively. No significant difference was observed in other cytokine and chemokine signatures between the two groups. In conclusion, our results revealed characteristic transcriptome and cytokine changes in hepatitis B vaccine non-responders after boost immunization.http://dx.doi.org/10.1080/21645515.2018.1450122hepatitis bvaccinenon-respondermicroarraytranscriptomecytokine |
| spellingShingle | Shaohui Qiu Peng He Xin Fang Haiqing Tong Jingjing Lv Jiaye Liu Li Zhang Xiangjun Zhai Liying Wang Zhongyu Hu Yongli Yu Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysis Human Vaccines & Immunotherapeutics hepatitis b vaccine non-responder microarray transcriptome cytokine |
| title | Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysis |
| title_full | Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysis |
| title_fullStr | Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysis |
| title_full_unstemmed | Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysis |
| title_short | Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysis |
| title_sort | significant transcriptome and cytokine changes in hepatitis b vaccine non responders revealed by genome wide comparative analysis |
| topic | hepatitis b vaccine non-responder microarray transcriptome cytokine |
| url | http://dx.doi.org/10.1080/21645515.2018.1450122 |
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