In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2)
Dengue virus (DENV), a member of the family Flaviviridae, is a threat for global health as it infects more than 100 million people yearly. Approved antiviral therapies or vaccines for the treatment or prevention of DENV infections are not available. In the present study, natural compounds were scree...
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/10/3016 |
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| author | Kingshuk Panda Kalichamy Alagarasu Poonam Patil Megha Agrawal Ashwini More Naveen V. Kumar Prathama S. Mainkar Deepti Parashar Sarah Cherian |
| author_facet | Kingshuk Panda Kalichamy Alagarasu Poonam Patil Megha Agrawal Ashwini More Naveen V. Kumar Prathama S. Mainkar Deepti Parashar Sarah Cherian |
| author_sort | Kingshuk Panda |
| collection | DOAJ |
| description | Dengue virus (DENV), a member of the family Flaviviridae, is a threat for global health as it infects more than 100 million people yearly. Approved antiviral therapies or vaccines for the treatment or prevention of DENV infections are not available. In the present study, natural compounds were screened for their antiviral activity against DENV by in vitro cell line-based assay. α-Mangostin, a xanthanoid, was observed to exert antiviral activity against DENV-2 under pre-, co- and post-treatment testing conditions. The antiviral activity was determined by foci forming unit (FFU) assay, quantitative RT-PCR and cell-based immunofluorescence assay (IFA). A complete inhibition of DENV-2 was observed at 8 µM under the co-treatment condition. The possible inhibitory mechanism of α-Mangostin was also determined by docking studies. The molecular docking experiments indicate that α-Mangostin can interact with multiple DENV protein targets such as the NS5 methyltransferase, NS2B-NS3 protease and the glycoprotein E. The in vitro and in silico findings suggest that α-Mangostin possesses the ability to suppress DENV-2 production at different stages of its replication cycle and might act as a prophylactic/therapeutic agent against DENV-2. |
| first_indexed | 2024-03-10T11:17:14Z |
| format | Article |
| id | doaj.art-920664ad328344c3997cf1a4465700ca |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:17:14Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-920664ad328344c3997cf1a4465700ca2023-11-21T20:21:10ZengMDPI AGMolecules1420-30492021-05-012610301610.3390/molecules26103016In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2)Kingshuk Panda0Kalichamy Alagarasu1Poonam Patil2Megha Agrawal3Ashwini More4Naveen V. Kumar5Prathama S. Mainkar6Deepti Parashar7Sarah Cherian8ICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, Maharashtra, IndiaICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, Maharashtra, IndiaICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, Maharashtra, IndiaICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, Maharashtra, IndiaICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, Maharashtra, IndiaCSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500007, Telangana, IndiaCSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500007, Telangana, IndiaICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, Maharashtra, IndiaICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, Maharashtra, IndiaDengue virus (DENV), a member of the family Flaviviridae, is a threat for global health as it infects more than 100 million people yearly. Approved antiviral therapies or vaccines for the treatment or prevention of DENV infections are not available. In the present study, natural compounds were screened for their antiviral activity against DENV by in vitro cell line-based assay. α-Mangostin, a xanthanoid, was observed to exert antiviral activity against DENV-2 under pre-, co- and post-treatment testing conditions. The antiviral activity was determined by foci forming unit (FFU) assay, quantitative RT-PCR and cell-based immunofluorescence assay (IFA). A complete inhibition of DENV-2 was observed at 8 µM under the co-treatment condition. The possible inhibitory mechanism of α-Mangostin was also determined by docking studies. The molecular docking experiments indicate that α-Mangostin can interact with multiple DENV protein targets such as the NS5 methyltransferase, NS2B-NS3 protease and the glycoprotein E. The in vitro and in silico findings suggest that α-Mangostin possesses the ability to suppress DENV-2 production at different stages of its replication cycle and might act as a prophylactic/therapeutic agent against DENV-2.https://www.mdpi.com/1420-3049/26/10/3016antiviralα-MangostindenguexanthonoidsFFU assayquantitative RT-PCR |
| spellingShingle | Kingshuk Panda Kalichamy Alagarasu Poonam Patil Megha Agrawal Ashwini More Naveen V. Kumar Prathama S. Mainkar Deepti Parashar Sarah Cherian In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) Molecules antiviral α-Mangostin dengue xanthonoids FFU assay quantitative RT-PCR |
| title | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
| title_full | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
| title_fullStr | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
| title_full_unstemmed | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
| title_short | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
| title_sort | in vitro antiviral activity of α mangostin against dengue virus serotype 2 denv 2 |
| topic | antiviral α-Mangostin dengue xanthonoids FFU assay quantitative RT-PCR |
| url | https://www.mdpi.com/1420-3049/26/10/3016 |
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