INDUÇÃO E REGENERAÇÃO DE EMBRIÕES SOMÁTICOS DE CANA-DE-AÇÚCAR RB996961
Sugarcane is a crop widely planted in Brazil because of the economic importance of its products, like sugar and ethanol. Thus, the crop is often inserted in breeding programs. Therefor, the aim of this study was to evaluate the morphogenic response to somatic embryogenesis and somatic embryos regene...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual do Oeste do Paraná
2015-09-01
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| Series: | Scientia Agraria Paranaensis |
| Subjects: | |
| Online Access: | http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9081 |
| _version_ | 1819041541002362880 |
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| author | Rodrigo de Oliveira Almeida Sérgio Delmar dos Anjos e Silva Daiane Peixoto Vargas Leonardo Ferreira Dutra |
| author_facet | Rodrigo de Oliveira Almeida Sérgio Delmar dos Anjos e Silva Daiane Peixoto Vargas Leonardo Ferreira Dutra |
| author_sort | Rodrigo de Oliveira Almeida |
| collection | DOAJ |
| description | Sugarcane is a crop widely planted in Brazil because of the economic importance of its products, like sugar and ethanol. Thus, the crop is often inserted in breeding programs. Therefor, the aim of this study was to evaluate the morphogenic response to somatic embryogenesis and somatic embryos regeneration for genotype RB996961. Transversal segments of young leave rolls with 2-3 mm size from plants with 6 to 9 months old were subcultured on solid MS medium supplemented with three concentrations of 2.4-dichlorophenoxyacetic acid (2.4-D) for embryogenic callus induction. Better results were obtained using 13.5 or 31.5 µM L-1 of 2.4-D in 45 or 60 days of incubation on induction medium, one to three subculture and 15 to 45 days of incubation on regeneration medium for genotype RB996961. This results show that the protocol used to genotype RB996961 was efficient to somatic embryos production (over 80%), nevertheless, it is necessary to adjust the regeneration approach (62.3 to 73.4%). |
| first_indexed | 2024-12-21T09:26:38Z |
| format | Article |
| id | doaj.art-9208bdbc0b494e2290279dcfc8923417 |
| institution | Directory Open Access Journal |
| issn | 1983-1471 |
| language | English |
| last_indexed | 2024-12-21T09:26:38Z |
| publishDate | 2015-09-01 |
| publisher | Universidade Estadual do Oeste do Paraná |
| record_format | Article |
| series | Scientia Agraria Paranaensis |
| spelling | doaj.art-9208bdbc0b494e2290279dcfc89234172022-12-21T19:08:53ZengUniversidade Estadual do Oeste do ParanáScientia Agraria Paranaensis1983-14712015-09-0114315415910.18188/1983-1471/sap.v14n3p154-159INDUÇÃO E REGENERAÇÃO DE EMBRIÕES SOMÁTICOS DE CANA-DE-AÇÚCAR RB996961Rodrigo de Oliveira Almeida0Sérgio Delmar dos Anjos e Silva1Daiane Peixoto Vargas2Leonardo Ferreira Dutra3Instituto Federal de Educação, Ciência e Tecnologia do Sudeste de Minas GeraisEmbrapa Clima TemperadoEmbrapa Clima TemperadoEmbrapa Clima TemperadoSugarcane is a crop widely planted in Brazil because of the economic importance of its products, like sugar and ethanol. Thus, the crop is often inserted in breeding programs. Therefor, the aim of this study was to evaluate the morphogenic response to somatic embryogenesis and somatic embryos regeneration for genotype RB996961. Transversal segments of young leave rolls with 2-3 mm size from plants with 6 to 9 months old were subcultured on solid MS medium supplemented with three concentrations of 2.4-dichlorophenoxyacetic acid (2.4-D) for embryogenic callus induction. Better results were obtained using 13.5 or 31.5 µM L-1 of 2.4-D in 45 or 60 days of incubation on induction medium, one to three subculture and 15 to 45 days of incubation on regeneration medium for genotype RB996961. This results show that the protocol used to genotype RB996961 was efficient to somatic embryos production (over 80%), nevertheless, it is necessary to adjust the regeneration approach (62.3 to 73.4%).http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9081Saccharum spp.plant tissue culturesomatic embriogenesis. |
| spellingShingle | Rodrigo de Oliveira Almeida Sérgio Delmar dos Anjos e Silva Daiane Peixoto Vargas Leonardo Ferreira Dutra INDUÇÃO E REGENERAÇÃO DE EMBRIÕES SOMÁTICOS DE CANA-DE-AÇÚCAR RB996961 Scientia Agraria Paranaensis Saccharum spp. plant tissue culture somatic embriogenesis. |
| title | INDUÇÃO E REGENERAÇÃO DE EMBRIÕES SOMÁTICOS DE CANA-DE-AÇÚCAR RB996961 |
| title_full | INDUÇÃO E REGENERAÇÃO DE EMBRIÕES SOMÁTICOS DE CANA-DE-AÇÚCAR RB996961 |
| title_fullStr | INDUÇÃO E REGENERAÇÃO DE EMBRIÕES SOMÁTICOS DE CANA-DE-AÇÚCAR RB996961 |
| title_full_unstemmed | INDUÇÃO E REGENERAÇÃO DE EMBRIÕES SOMÁTICOS DE CANA-DE-AÇÚCAR RB996961 |
| title_short | INDUÇÃO E REGENERAÇÃO DE EMBRIÕES SOMÁTICOS DE CANA-DE-AÇÚCAR RB996961 |
| title_sort | inducao e regeneracao de embrioes somaticos de cana de acucar rb996961 |
| topic | Saccharum spp. plant tissue culture somatic embriogenesis. |
| url | http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9081 |
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