A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode
A simple method that relies only on an electrochemical workstation has been investigated to fabricate a highly sensitive glutamate microbiosensor for potential neuroscience applications. In this study, in order to develop the highly sensitive glutamate electrode, a 100 µm platinum wire was modified...
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MDPI AG
2020-05-01
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author | Jing Chen Qiwen Yu Wei Fu Xing Chen Quan Zhang Shurong Dong Hang Chen Shaomin Zhang |
author_facet | Jing Chen Qiwen Yu Wei Fu Xing Chen Quan Zhang Shurong Dong Hang Chen Shaomin Zhang |
author_sort | Jing Chen |
collection | DOAJ |
description | A simple method that relies only on an electrochemical workstation has been investigated to fabricate a highly sensitive glutamate microbiosensor for potential neuroscience applications. In this study, in order to develop the highly sensitive glutamate electrode, a 100 µm platinum wire was modified by the electrochemical deposition of gold nanoparticles, Prussian blue nanocubes, and reduced graphene oxide sheets, which increased the electroactive surface area; and the chitosan layer, which provided a suitable environment to bond the glutamate oxidase. The optimization of the fabrication procedure and analytical conditions is described. The modified electrode was characterized using field emission scanning electron microscopy, impedance spectroscopy, and cyclic voltammetry. The results exhibited its excellent sensitivity for glutamate detection (LOD = 41.33 nM), adequate linearity (50 nM–40 µM), ascendant reproducibility (RSD = 4.44%), and prolonged stability (more than 30 repetitive potential sweeps, two-week lifespan). Because of the important role of glutamate in neurotransmission and brain function, this small-dimension, high-sensitivity glutamate electrode is a promising tool in neuroscience research. |
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spelling | doaj.art-920a83ba9b4449edb7a7abd7d4894cbc2023-11-20T01:16:28ZengMDPI AGSensors1424-82202020-05-012010292410.3390/s20102924A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt ElectrodeJing Chen0Qiwen Yu1Wei Fu2Xing Chen3Quan Zhang4Shurong Dong5Hang Chen6Shaomin Zhang7Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaKey Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaKey Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaKey Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaNeural Systems Group, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USAZhejiang Laboratory, Hangzhou 310027, ChinaKey Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaKey Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaA simple method that relies only on an electrochemical workstation has been investigated to fabricate a highly sensitive glutamate microbiosensor for potential neuroscience applications. In this study, in order to develop the highly sensitive glutamate electrode, a 100 µm platinum wire was modified by the electrochemical deposition of gold nanoparticles, Prussian blue nanocubes, and reduced graphene oxide sheets, which increased the electroactive surface area; and the chitosan layer, which provided a suitable environment to bond the glutamate oxidase. The optimization of the fabrication procedure and analytical conditions is described. The modified electrode was characterized using field emission scanning electron microscopy, impedance spectroscopy, and cyclic voltammetry. The results exhibited its excellent sensitivity for glutamate detection (LOD = 41.33 nM), adequate linearity (50 nM–40 µM), ascendant reproducibility (RSD = 4.44%), and prolonged stability (more than 30 repetitive potential sweeps, two-week lifespan). Because of the important role of glutamate in neurotransmission and brain function, this small-dimension, high-sensitivity glutamate electrode is a promising tool in neuroscience research.https://www.mdpi.com/1424-8220/20/10/2924microbiosensorglutamateglutamate oxidasePrussian blue nanocubeschitosanamperometry |
spellingShingle | Jing Chen Qiwen Yu Wei Fu Xing Chen Quan Zhang Shurong Dong Hang Chen Shaomin Zhang A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode Sensors microbiosensor glutamate glutamate oxidase Prussian blue nanocubes chitosan amperometry |
title | A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode |
title_full | A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode |
title_fullStr | A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode |
title_full_unstemmed | A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode |
title_short | A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode |
title_sort | highly sensitive amperometric glutamate oxidase microbiosensor based on a reduced graphene oxide prussian blue nanocube gold nanoparticle composite film modified pt electrode |
topic | microbiosensor glutamate glutamate oxidase Prussian blue nanocubes chitosan amperometry |
url | https://www.mdpi.com/1424-8220/20/10/2924 |
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