Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, India
Background: Food-borne pathogen Listeria monocytogenes is abundantly present in nature and accountable for sporadic and epidemic cases of listeriosis in humans. The objective of this study was to screen common food sources for L. monocytogenes using biochemical and molecular methods to detect and ch...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Journal of Infection and Public Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034124000467 |
| _version_ | 1797249158517620736 |
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| author | Roshan Kumar Sharma Sunil S. Jalalpure Swati Pathak Sachit Ganapathy Mickaël Desvaux Subarna Roy Satisha Hegde |
| author_facet | Roshan Kumar Sharma Sunil S. Jalalpure Swati Pathak Sachit Ganapathy Mickaël Desvaux Subarna Roy Satisha Hegde |
| author_sort | Roshan Kumar Sharma |
| collection | DOAJ |
| description | Background: Food-borne pathogen Listeria monocytogenes is abundantly present in nature and accountable for sporadic and epidemic cases of listeriosis in humans. The objective of this study was to screen common food sources for L. monocytogenes using biochemical and molecular methods to detect and characterise its toxin genes as well as for biofilm formation. Methods: A total of 92 samples, comprising dairy and street food products, were randomly collected from various sources for this investigation. The collected samples were processed for biochemical and molecular methods to detect L. monocytogenes. Additionally, virulence factors associated genes, antibiogram profiles and biofilm formation related assays were determined. Results: L. monocytogenes presence was confirmed using molecular detection methods targeting prs and lmo1030 genes, along with MALDI-TOF MS. Following 16 S rRNA sequencing, the identified Listeria species were further categorised into two groups. L. monocytogenes was detected in two (2.17%) food samples tested (L-23 and L-74). Multiplex PCR indicated the presence of seven virulence-related genes in L. monocytogenes isolates, i.e., inlA, inlB, prfA, iap, actA, plcB, and hlyA. In addition, 17 antibiotics were tested, whereby two isolates showed resistance to clindamycin and azithromycin, while one isolate (L-74) was also resistant to nalidixic acid, co-trimoxazole, ampicillin, norfloxacin, and cefotaxime. L-23 and L-74 isolates showed biofilm formation, especially at pH 8.6 and 37°C. Conclusions: Besides the demonstration of the presence of L. monocytogenes in some dairy and street food products, this study underscores the need to increase the standards of hygiene on the one hand and the importance of the surveillance of food-borne pathogens on the other. |
| first_indexed | 2024-04-24T20:26:02Z |
| format | Article |
| id | doaj.art-920bace07d0c4e3eac4998865dc1cebd |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-04-24T20:26:02Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-920bace07d0c4e3eac4998865dc1cebd2024-03-22T05:39:01ZengElsevierJournal of Infection and Public Health1876-03412024-04-01174696703Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, IndiaRoshan Kumar Sharma0Sunil S. Jalalpure1Swati Pathak2Sachit Ganapathy3Mickaël Desvaux4Subarna Roy5Satisha Hegde6KLE Academy of Higher Education and Research, Dr. Prabhakar Kore Basic Science Research Centre, Belagavi 590010, IndiaKLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi 590010, IndiaInstitute of Medical Sciences, Banaras Hindu University, Varanasi 221005, IndiaDepartment of Biostatistics, Jawaharlal Institute of Post Graduate Medical Education and Research (JIPMER), Puducherry 605006, IndiaINRAE, UCA, UMR0454 MEDIS, 63000 Clermont-Ferrand, FranceICMR - National Institute of Traditional Medicine (ICMR-NITM), Department of Health Research, Govt. of India, Belagavi 590010, IndiaKLE Academy of Higher Education and Research, Dr. Prabhakar Kore Basic Science Research Centre, Belagavi 590010, India; Corresponding author.Background: Food-borne pathogen Listeria monocytogenes is abundantly present in nature and accountable for sporadic and epidemic cases of listeriosis in humans. The objective of this study was to screen common food sources for L. monocytogenes using biochemical and molecular methods to detect and characterise its toxin genes as well as for biofilm formation. Methods: A total of 92 samples, comprising dairy and street food products, were randomly collected from various sources for this investigation. The collected samples were processed for biochemical and molecular methods to detect L. monocytogenes. Additionally, virulence factors associated genes, antibiogram profiles and biofilm formation related assays were determined. Results: L. monocytogenes presence was confirmed using molecular detection methods targeting prs and lmo1030 genes, along with MALDI-TOF MS. Following 16 S rRNA sequencing, the identified Listeria species were further categorised into two groups. L. monocytogenes was detected in two (2.17%) food samples tested (L-23 and L-74). Multiplex PCR indicated the presence of seven virulence-related genes in L. monocytogenes isolates, i.e., inlA, inlB, prfA, iap, actA, plcB, and hlyA. In addition, 17 antibiotics were tested, whereby two isolates showed resistance to clindamycin and azithromycin, while one isolate (L-74) was also resistant to nalidixic acid, co-trimoxazole, ampicillin, norfloxacin, and cefotaxime. L-23 and L-74 isolates showed biofilm formation, especially at pH 8.6 and 37°C. Conclusions: Besides the demonstration of the presence of L. monocytogenes in some dairy and street food products, this study underscores the need to increase the standards of hygiene on the one hand and the importance of the surveillance of food-borne pathogens on the other.http://www.sciencedirect.com/science/article/pii/S1876034124000467DetectionFoodDNA SequencingListeriaMolecularPathogens |
| spellingShingle | Roshan Kumar Sharma Sunil S. Jalalpure Swati Pathak Sachit Ganapathy Mickaël Desvaux Subarna Roy Satisha Hegde Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, India Journal of Infection and Public Health Detection Food DNA Sequencing Listeria Molecular Pathogens |
| title | Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, India |
| title_full | Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, India |
| title_fullStr | Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, India |
| title_full_unstemmed | Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, India |
| title_short | Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, India |
| title_sort | molecular detection of listeria monocytogenes from different dairy and street food sources in north karnataka india |
| topic | Detection Food DNA Sequencing Listeria Molecular Pathogens |
| url | http://www.sciencedirect.com/science/article/pii/S1876034124000467 |
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