Decorin promotes robust axon growth on inhibitory CSPGs and myelin via a direct effect on neurons
Inhibitory chondroitin sulfate proteoglycans (CSPGs) and myelin-associated molecules are major impediments to axon regeneration within the adult central nervous system (CNS). Decorin infusion can however suppress the levels of multiple inhibitory CSPGs and promote axon growth across spinal cord inju...
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| Format: | Article |
| Language: | English |
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Elsevier
2008-10-01
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| Series: | Neurobiology of Disease |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996108001344 |
| _version_ | 1818414563724361728 |
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| author | Kenneth Minor Xiufeng Tang Genevieve Kahrilas Simon J. Archibald Jeannette E. Davies Stephen J. Davies |
| author_facet | Kenneth Minor Xiufeng Tang Genevieve Kahrilas Simon J. Archibald Jeannette E. Davies Stephen J. Davies |
| author_sort | Kenneth Minor |
| collection | DOAJ |
| description | Inhibitory chondroitin sulfate proteoglycans (CSPGs) and myelin-associated molecules are major impediments to axon regeneration within the adult central nervous system (CNS). Decorin infusion can however suppress the levels of multiple inhibitory CSPGs and promote axon growth across spinal cord injuries [Davies, J.E., Tang, X., Denning, J.W., Archibald, S.J., and Davies, S.J., 2004. Decorin suppresses neurocan, brevican, phosphacan and NG2 expression and promotes axon growth across adult rat spinal cord injuries. Eur. J. Neurosci. 19, 1226–1242]. A question remained as to whether decorin can also increase axon growth on inhibitory CSPGs and myelin via a direct effect on neurons. We have therefore conducted an in vitro analysis of neurite extension by decorin-treated adult dorsal root ganglion (DRG) neurons cultured on substrates of inhibitory CSPGs or myelin membranes mixed with laminin. Decorin treatment promoted 14.5 and 5-fold increases in average neurite length/neuron over untreated controls on CSPGs or myelin membranes respectively. In addition to suppressing inhibitory scar formation, our present data shows that decorin can directly boost the ability of neurons to extend axons within CSPG or myelin rich environments. |
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| format | Article |
| id | doaj.art-920c8089d4e04ce6a4849ee217d3f150 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-14T11:21:05Z |
| publishDate | 2008-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-920c8089d4e04ce6a4849ee217d3f1502022-12-21T23:03:46ZengElsevierNeurobiology of Disease1095-953X2008-10-013218895Decorin promotes robust axon growth on inhibitory CSPGs and myelin via a direct effect on neuronsKenneth Minor0Xiufeng Tang1Genevieve Kahrilas2Simon J. Archibald3Jeannette E. Davies4Stephen J. Davies5Department of Neurosurgery, Anschutz Medical Campus, University of Colorado at Denver, Neurosurgery Research Laboratory, Building RC-1 North, Room P18-9100, 12800 East 19th Avenue, Aurora, CO 80045, USAMethodist Neurological Institute, Department of Neurosurgery, Scurlock Tower Suite 944, 6560 Fannin Street, Houston, TX 77030, USADepartment of Neurosurgery, Anschutz Medical Campus, University of Colorado at Denver, Neurosurgery Research Laboratory, Building RC-1 North, Room P18-9100, 12800 East 19th Avenue, Aurora, CO 80045, USAIntegra LifeSciences, 311C Enterprise Drive, Plainsboro, NJ, USADepartment of Neurosurgery, Anschutz Medical Campus, University of Colorado at Denver, Neurosurgery Research Laboratory, Building RC-1 North, Room P18-9100, 12800 East 19th Avenue, Aurora, CO 80045, USADepartment of Neurosurgery, Anschutz Medical Campus, University of Colorado at Denver, Neurosurgery Research Laboratory, Building RC-1 North, Room P18-9100, 12800 East 19th Avenue, Aurora, CO 80045, USA; Corresponding author. Fax: +1 303 724 3817.Inhibitory chondroitin sulfate proteoglycans (CSPGs) and myelin-associated molecules are major impediments to axon regeneration within the adult central nervous system (CNS). Decorin infusion can however suppress the levels of multiple inhibitory CSPGs and promote axon growth across spinal cord injuries [Davies, J.E., Tang, X., Denning, J.W., Archibald, S.J., and Davies, S.J., 2004. Decorin suppresses neurocan, brevican, phosphacan and NG2 expression and promotes axon growth across adult rat spinal cord injuries. Eur. J. Neurosci. 19, 1226–1242]. A question remained as to whether decorin can also increase axon growth on inhibitory CSPGs and myelin via a direct effect on neurons. We have therefore conducted an in vitro analysis of neurite extension by decorin-treated adult dorsal root ganglion (DRG) neurons cultured on substrates of inhibitory CSPGs or myelin membranes mixed with laminin. Decorin treatment promoted 14.5 and 5-fold increases in average neurite length/neuron over untreated controls on CSPGs or myelin membranes respectively. In addition to suppressing inhibitory scar formation, our present data shows that decorin can directly boost the ability of neurons to extend axons within CSPG or myelin rich environments.http://www.sciencedirect.com/science/article/pii/S0969996108001344DecorinChondroitin surfate proteoglycansMyelin associated inhibitorsMagNOGO-AOMgp |
| spellingShingle | Kenneth Minor Xiufeng Tang Genevieve Kahrilas Simon J. Archibald Jeannette E. Davies Stephen J. Davies Decorin promotes robust axon growth on inhibitory CSPGs and myelin via a direct effect on neurons Neurobiology of Disease Decorin Chondroitin surfate proteoglycans Myelin associated inhibitors Mag NOGO-A OMgp |
| title | Decorin promotes robust axon growth on inhibitory CSPGs and myelin via a direct effect on neurons |
| title_full | Decorin promotes robust axon growth on inhibitory CSPGs and myelin via a direct effect on neurons |
| title_fullStr | Decorin promotes robust axon growth on inhibitory CSPGs and myelin via a direct effect on neurons |
| title_full_unstemmed | Decorin promotes robust axon growth on inhibitory CSPGs and myelin via a direct effect on neurons |
| title_short | Decorin promotes robust axon growth on inhibitory CSPGs and myelin via a direct effect on neurons |
| title_sort | decorin promotes robust axon growth on inhibitory cspgs and myelin via a direct effect on neurons |
| topic | Decorin Chondroitin surfate proteoglycans Myelin associated inhibitors Mag NOGO-A OMgp |
| url | http://www.sciencedirect.com/science/article/pii/S0969996108001344 |
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