Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses
Laser-induced shockwaves (LIS) can be utilized as a method to subject cells to conditions similar to those occurring during a blast-induced traumatic brain injury. The pairing of LIS with genetically encoded biosensors allows researchers to monitor the immediate molecular events resulting from such...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.598896/full |
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| author | Veronica Gomez Godinez Vikash Morar Christopher Carmona Yingli Gu Kijung Sung Linda Z. Shi Chengbiao Wu Daryl Preece Daryl Preece Michael W. Berns Michael W. Berns Michael W. Berns |
| author_facet | Veronica Gomez Godinez Vikash Morar Christopher Carmona Yingli Gu Kijung Sung Linda Z. Shi Chengbiao Wu Daryl Preece Daryl Preece Michael W. Berns Michael W. Berns Michael W. Berns |
| author_sort | Veronica Gomez Godinez |
| collection | DOAJ |
| description | Laser-induced shockwaves (LIS) can be utilized as a method to subject cells to conditions similar to those occurring during a blast-induced traumatic brain injury. The pairing of LIS with genetically encoded biosensors allows researchers to monitor the immediate molecular events resulting from such an injury. In this study, we utilized the genetically encoded Ca2+ FRET biosensor D3CPV to study the immediate Ca2+ response to laser-induced shockwave in cortical neurons and Schwann cells. Our results show that both cell types exhibit a transient Ca2+ increase irrespective of extracellular Ca2+ conditions. LIS allows for the simultaneous monitoring of the effects of shear stress on cells, as well as nearby cell damage and death. |
| first_indexed | 2024-12-14T23:47:10Z |
| format | Article |
| id | doaj.art-920ee3d1862a49d794d75ff90d2dab8c |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-14T23:47:10Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-920ee3d1862a49d794d75ff90d2dab8c2022-12-21T22:43:20ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-02-01910.3389/fbioe.2021.598896598896Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ ResponsesVeronica Gomez Godinez0Vikash Morar1Christopher Carmona2Yingli Gu3Kijung Sung4Linda Z. Shi5Chengbiao Wu6Daryl Preece7Daryl Preece8Michael W. Berns9Michael W. Berns10Michael W. Berns11Institute of Engineering in Medicine, University of California, San Diego, San Diego, CA, United StatesInstitute of Engineering in Medicine, University of California, San Diego, San Diego, CA, United StatesInstitute of Engineering in Medicine, University of California, San Diego, San Diego, CA, United StatesDepartment of Neurosciences, University of California, San Diego, San Diego, CA, United StatesDepartment of Neurosciences, University of California, San Diego, San Diego, CA, United StatesInstitute of Engineering in Medicine, University of California, San Diego, San Diego, CA, United StatesDepartment of Neurosciences, University of California, San Diego, San Diego, CA, United StatesBeckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, CA, United StatesDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, United StatesBeckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, CA, United StatesDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, United StatesDepartment of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, Irvine, CA, United StatesLaser-induced shockwaves (LIS) can be utilized as a method to subject cells to conditions similar to those occurring during a blast-induced traumatic brain injury. The pairing of LIS with genetically encoded biosensors allows researchers to monitor the immediate molecular events resulting from such an injury. In this study, we utilized the genetically encoded Ca2+ FRET biosensor D3CPV to study the immediate Ca2+ response to laser-induced shockwave in cortical neurons and Schwann cells. Our results show that both cell types exhibit a transient Ca2+ increase irrespective of extracellular Ca2+ conditions. LIS allows for the simultaneous monitoring of the effects of shear stress on cells, as well as nearby cell damage and death.https://www.frontiersin.org/articles/10.3389/fbioe.2021.598896/fullneuronal calciumcavitation bubbletraumatic brain injuryshockwaveblast induced traumalaser induced shockwave |
| spellingShingle | Veronica Gomez Godinez Vikash Morar Christopher Carmona Yingli Gu Kijung Sung Linda Z. Shi Chengbiao Wu Daryl Preece Daryl Preece Michael W. Berns Michael W. Berns Michael W. Berns Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses Frontiers in Bioengineering and Biotechnology neuronal calcium cavitation bubble traumatic brain injury shockwave blast induced trauma laser induced shockwave |
| title | Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses |
| title_full | Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses |
| title_fullStr | Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses |
| title_full_unstemmed | Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses |
| title_short | Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses |
| title_sort | laser induced shockwave lis to study neuronal ca2 responses |
| topic | neuronal calcium cavitation bubble traumatic brain injury shockwave blast induced trauma laser induced shockwave |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.598896/full |
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