Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case Study
The aim of this work was to inspect the presence of asbestos fibers in colon tissue from a patient, with history of indirect exposure to asbestos and affected by colon cancer, who underwent surgery. Variable pressure scanning electron microscopy, coupled with energy dispersive spectroscopy (VP-SEM/E...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Minerals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-163X/11/6/658 |
| _version_ | 1797529300513062912 |
|---|---|
| author | Caterina Rinaudo Alessandro Croce Stefania Erra Elisabetta Nada Marinella Bertolotti Federica Grosso Antonio Maconi Marco Amisano |
| author_facet | Caterina Rinaudo Alessandro Croce Stefania Erra Elisabetta Nada Marinella Bertolotti Federica Grosso Antonio Maconi Marco Amisano |
| author_sort | Caterina Rinaudo |
| collection | DOAJ |
| description | The aim of this work was to inspect the presence of asbestos fibers in colon tissue from a patient, with history of indirect exposure to asbestos and affected by colon cancer, who underwent surgery. Variable pressure scanning electron microscopy, coupled with energy dispersive spectroscopy (VP-SEM/EDS), was used for identification of inorganic fibers and for their morphological—chemical characterization. Fresh tissue samples from both, healthy area close to the neoplasia and from the neoplastic regions, were separately digested to eliminate the biological matrix. The precipitate was analyzed by VP-SEM/EDS, identifying in samples from healthy tissue asbestos bodies and small asbestos fibers, and in samples from neoplastic tissue long fibers of asbestos, free from covering. A quantification of the asbestos bodies and the free fibers in the two type of specimens is proposed. Moreover, to locate the fibers in the biological medium, histological sections from the colon of the same patient were also examined. Free asbestos fibers appeared concentrated in the tissue bridge between the healthy and the neoplastic areas. Immuno-histochemical investigation performed on the neoplasia seems to exclude a role of microsatellite instability in the carcinogenesis process, suggesting an influence of the fibers. |
| first_indexed | 2024-03-10T10:12:48Z |
| format | Article |
| id | doaj.art-920fdfcb17534778b2559ffe1fd44e84 |
| institution | Directory Open Access Journal |
| issn | 2075-163X |
| language | English |
| last_indexed | 2024-03-10T10:12:48Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Minerals |
| spelling | doaj.art-920fdfcb17534778b2559ffe1fd44e842023-11-22T01:06:00ZengMDPI AGMinerals2075-163X2021-06-0111665810.3390/min11060658Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case StudyCaterina Rinaudo0Alessandro Croce1Stefania Erra2Elisabetta Nada3Marinella Bertolotti4Federica Grosso5Antonio Maconi6Marco Amisano7Department of Science and Technological Innovation, University of Eastern Piedmont, Viale T. Michel 11, 15121 Alessandria, ItalyDepartment of Science and Technological Innovation, University of Eastern Piedmont, Viale T. Michel 11, 15121 Alessandria, ItalyDepartment of Anatomy and Pathology, S. Spirito Hospital, Viale Giolitti 2, 15033 Casale Monferrato, ItalyDepartment of General Surgery, S. Spirito Hospital, Viale Giolitti 2, 15033 Casale Monferrato, ItalyInfrastruttura Ricerca Formazione Innovazione, Dipartimento Attività Integrate Ricerca Innovazione, Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo, Via Venezia 16, 15121 Alessandria, ItalyMesothelioma Unit, Oncology, SS Antonio e Biagio e Cesare Arrigo, General Hospital, Via Venezia 16, 15121 Alessandria, ItalyInfrastruttura Ricerca Formazione Innovazione, Dipartimento Attività Integrate Ricerca Innovazione, Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo, Via Venezia 16, 15121 Alessandria, ItalyDepartment of General Surgery, S. Spirito Hospital, Viale Giolitti 2, 15033 Casale Monferrato, ItalyThe aim of this work was to inspect the presence of asbestos fibers in colon tissue from a patient, with history of indirect exposure to asbestos and affected by colon cancer, who underwent surgery. Variable pressure scanning electron microscopy, coupled with energy dispersive spectroscopy (VP-SEM/EDS), was used for identification of inorganic fibers and for their morphological—chemical characterization. Fresh tissue samples from both, healthy area close to the neoplasia and from the neoplastic regions, were separately digested to eliminate the biological matrix. The precipitate was analyzed by VP-SEM/EDS, identifying in samples from healthy tissue asbestos bodies and small asbestos fibers, and in samples from neoplastic tissue long fibers of asbestos, free from covering. A quantification of the asbestos bodies and the free fibers in the two type of specimens is proposed. Moreover, to locate the fibers in the biological medium, histological sections from the colon of the same patient were also examined. Free asbestos fibers appeared concentrated in the tissue bridge between the healthy and the neoplastic areas. Immuno-histochemical investigation performed on the neoplasia seems to exclude a role of microsatellite instability in the carcinogenesis process, suggesting an influence of the fibers.https://www.mdpi.com/2075-163X/11/6/658asbestosferruginous bodiescolon cancersVP-SEM/EDS |
| spellingShingle | Caterina Rinaudo Alessandro Croce Stefania Erra Elisabetta Nada Marinella Bertolotti Federica Grosso Antonio Maconi Marco Amisano Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case Study Minerals asbestos ferruginous bodies colon cancers VP-SEM/EDS |
| title | Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case Study |
| title_full | Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case Study |
| title_fullStr | Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case Study |
| title_full_unstemmed | Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case Study |
| title_short | Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case Study |
| title_sort | asbestos fibers and ferruginous bodies detected by vp sem eds in colon tissues of a patient affected by colon rectum cancer a case study |
| topic | asbestos ferruginous bodies colon cancers VP-SEM/EDS |
| url | https://www.mdpi.com/2075-163X/11/6/658 |
| work_keys_str_mv | AT caterinarinaudo asbestosfibersandferruginousbodiesdetectedbyvpsemedsincolontissuesofapatientaffectedbycolonrectumcanceracasestudy AT alessandrocroce asbestosfibersandferruginousbodiesdetectedbyvpsemedsincolontissuesofapatientaffectedbycolonrectumcanceracasestudy AT stefaniaerra asbestosfibersandferruginousbodiesdetectedbyvpsemedsincolontissuesofapatientaffectedbycolonrectumcanceracasestudy AT elisabettanada asbestosfibersandferruginousbodiesdetectedbyvpsemedsincolontissuesofapatientaffectedbycolonrectumcanceracasestudy AT marinellabertolotti asbestosfibersandferruginousbodiesdetectedbyvpsemedsincolontissuesofapatientaffectedbycolonrectumcanceracasestudy AT federicagrosso asbestosfibersandferruginousbodiesdetectedbyvpsemedsincolontissuesofapatientaffectedbycolonrectumcanceracasestudy AT antoniomaconi asbestosfibersandferruginousbodiesdetectedbyvpsemedsincolontissuesofapatientaffectedbycolonrectumcanceracasestudy AT marcoamisano asbestosfibersandferruginousbodiesdetectedbyvpsemedsincolontissuesofapatientaffectedbycolonrectumcanceracasestudy |