Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy
While extracellular vesicles (EVs) are extensively studied by various practical applications in biomedicine, there is still little information on their biomechanical properties due to their nanoscale size. We identified isolated blood plasma vesicles that carried on biomarkers associated with exosom...
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MDPI AG
2020-12-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/1/4 |
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| author | Viktor Bairamukov Anton Bukatin Sergey Landa Vladimir Burdakov Tatiana Shtam Irina Chelnokova Natalia Fedorova Michael Filatov Maria Starodubtseva |
| author_facet | Viktor Bairamukov Anton Bukatin Sergey Landa Vladimir Burdakov Tatiana Shtam Irina Chelnokova Natalia Fedorova Michael Filatov Maria Starodubtseva |
| author_sort | Viktor Bairamukov |
| collection | DOAJ |
| description | While extracellular vesicles (EVs) are extensively studied by various practical applications in biomedicine, there is still little information on their biomechanical properties due to their nanoscale size. We identified isolated blood plasma vesicles that carried on biomarkers associated with exosomes and exomeres and applied atomic force microscopy (AFM) to study them at single particle level in air and in liquid. Air measurements of exosomes revealed a mechanically indented internal cavity in which highly adhesive sites were located. In contrast, the highly adhesive sites of exomeres were located at the periphery and the observed diameter of the particles was ~35 nm. In liquid, the reversible deformation of the internal cavity of exosomes was observed and a slightly deformed lipid bi-layer was identified. In contrast, exomeres were not deformed and their observed diameter was ~16 nm. The difference in diameters might be associated with a higher sorption of water film in air. The parameters we revealed correlated with the well-known structure and function for exosomes and were observed for exomeres for the first time. Our data provide a new insight into the biomechanical properties of nanoparticles and positioned AFM as an exclusive source of in situ information about their biophysical characteristics. |
| first_indexed | 2024-03-10T13:51:07Z |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T13:51:07Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-92103fe43a904198b650f684800be6272023-11-21T02:09:02ZengMDPI AGBiology2079-77372020-12-01101410.3390/biology10010004Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force MicroscopyViktor Bairamukov0Anton Bukatin1Sergey Landa2Vladimir Burdakov3Tatiana Shtam4Irina Chelnokova5Natalia Fedorova6Michael Filatov7Maria Starodubtseva8Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 1, Orlova Roshcha, 188300 Gatchina, RussiaAlferov Saint Petersburg National Research Academic University of the Russian Academy of Sciences, 8/3, Khlopina St., 194021 Saint Petersburg, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 1, Orlova Roshcha, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 1, Orlova Roshcha, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 1, Orlova Roshcha, 188300 Gatchina, RussiaInstitute of Radiobiology of NAS of Belarus, 4, Fedyuninskogo St., 246007 Gomel, BelarusPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 1, Orlova Roshcha, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 1, Orlova Roshcha, 188300 Gatchina, RussiaInstitute of Radiobiology of NAS of Belarus, 4, Fedyuninskogo St., 246007 Gomel, BelarusWhile extracellular vesicles (EVs) are extensively studied by various practical applications in biomedicine, there is still little information on their biomechanical properties due to their nanoscale size. We identified isolated blood plasma vesicles that carried on biomarkers associated with exosomes and exomeres and applied atomic force microscopy (AFM) to study them at single particle level in air and in liquid. Air measurements of exosomes revealed a mechanically indented internal cavity in which highly adhesive sites were located. In contrast, the highly adhesive sites of exomeres were located at the periphery and the observed diameter of the particles was ~35 nm. In liquid, the reversible deformation of the internal cavity of exosomes was observed and a slightly deformed lipid bi-layer was identified. In contrast, exomeres were not deformed and their observed diameter was ~16 nm. The difference in diameters might be associated with a higher sorption of water film in air. The parameters we revealed correlated with the well-known structure and function for exosomes and were observed for exomeres for the first time. Our data provide a new insight into the biomechanical properties of nanoparticles and positioned AFM as an exclusive source of in situ information about their biophysical characteristics.https://www.mdpi.com/2079-7737/10/1/4extracellular vesiclesexosomesexomeresatomic-force microscopyquantitative nanomechanical mapping |
| spellingShingle | Viktor Bairamukov Anton Bukatin Sergey Landa Vladimir Burdakov Tatiana Shtam Irina Chelnokova Natalia Fedorova Michael Filatov Maria Starodubtseva Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy Biology extracellular vesicles exosomes exomeres atomic-force microscopy quantitative nanomechanical mapping |
| title | Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy |
| title_full | Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy |
| title_fullStr | Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy |
| title_full_unstemmed | Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy |
| title_short | Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy |
| title_sort | biomechanical properties of blood plasma extracellular vesicles revealed by atomic force microscopy |
| topic | extracellular vesicles exosomes exomeres atomic-force microscopy quantitative nanomechanical mapping |
| url | https://www.mdpi.com/2079-7737/10/1/4 |
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