Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower
The present study involved the bio-fabrication of silver nanoparticles (AgNPs) by using the <i>Euphorbia helioscopia</i> L. leaves aqueous extract to improve the production of secondary metabolites in industrially important sunflower (<i>Helianthus annuus</i> L.) plants. Phyt...
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MDPI AG
2021-07-01
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| Series: | Nanomaterials |
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| author | Syeda Umber Batool Bilal Javed Sohail Syeda Sadaf Zehra Zia-ur-Rehman Mashwani Naveed Iqbal Raja Tariq Khan Haifa Abdulaziz Sakit ALHaithloul Suliman Mohammed Alghanem Amina A. M. Al-Mushhin Mohamed Hashem Saad Alamri |
| author_facet | Syeda Umber Batool Bilal Javed Sohail Syeda Sadaf Zehra Zia-ur-Rehman Mashwani Naveed Iqbal Raja Tariq Khan Haifa Abdulaziz Sakit ALHaithloul Suliman Mohammed Alghanem Amina A. M. Al-Mushhin Mohamed Hashem Saad Alamri |
| author_sort | Syeda Umber Batool |
| collection | DOAJ |
| description | The present study involved the bio-fabrication of silver nanoparticles (AgNPs) by using the <i>Euphorbia helioscopia</i> L. leaves aqueous extract to improve the production of secondary metabolites in industrially important sunflower (<i>Helianthus annuus</i> L.) plants. Phyto-fabrication of AgNPs was confirmed by using spectrophotometry, SEM imaging and X-ray diffraction analysis. The morphological and optical characterization manifested that the AgNPs are crystalline and exist in the size range of 30–100 nm. Various concentrations (10, 20, 40, 60, 80 and 100 mg/L) of AgNPs were applied in combinations on sunflower seeds and crop plants. The effects of biosynthesized AgNPs were evaluated for agro-morphological parameters (plant height, flowering initiation and seed weight), biochemical metabolites (chlorophyll, proline, soluble sugar, amino acid and protein contents) and enzymatic activities (superoxide dismutase and ascorbate peroxidase) in sunflower and 60 mg/L concentration of AgNPs on sunflower seeds and foliar sprays on plants in combination were found to be effective to elicit biochemical modifications to improve secondary metabolites. It was also observed experimentally that 60 mg/L concentration of AgNPs improved the biochemical, fatty acid and enzymatic attributes of sunflower plants, which in turn improved the plant agro-morphological parameters. Near-infrared spectroscopic analysis results confirmed the improvement in the seed quality, oil contents and fatty acid composition (palmitic acid, oleic acid and linoleic acid) after the applications of AgNPs. The findings of the present investigation confirm the exogenous applications of bio-fabricated AgNPs in combinations on seeds and plants to improve the plant yield, seed quality and secondary metabolite contents of the sunflower plants. |
| first_indexed | 2024-03-10T09:30:40Z |
| format | Article |
| id | doaj.art-9210bbfed4cf425897b6884d8f6d5bca |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T09:30:40Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-9210bbfed4cf425897b6884d8f6d5bca2023-11-22T04:33:21ZengMDPI AGNanomaterials2079-49912021-07-01117175010.3390/nano11071750Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of SunflowerSyeda Umber Batool0Bilal Javed1Sohail2Syeda Sadaf Zehra3Zia-ur-Rehman Mashwani4Naveed Iqbal Raja5Tariq Khan6Haifa Abdulaziz Sakit ALHaithloul7Suliman Mohammed Alghanem8Amina A. M. Al-Mushhin9Mohamed Hashem10Saad Alamri11Department of Chemical and Life Sciences, Qurtuba University of Science & Information Technology, Peshawar 25000, Khyber Pakhtunkhwa, PakistanDepartment of Botany, PMAS Arid Agriculture University, Rawalpindi 46300, Punjab, PakistanInstitute of Biology/Plant Physiology, Humboldt-University Zü Berlin, 10115 Berlin, GermanyDepartment of Botany, The Islamia University of Bahawalpur, Bahawalpur 63100, Punjab, PakistanDepartment of Botany, PMAS Arid Agriculture University, Rawalpindi 46300, Punjab, PakistanDepartment of Botany, PMAS Arid Agriculture University, Rawalpindi 46300, Punjab, PakistanDepartment of Biotechnology, University of Malakand, Chakdara, Lower Dir 18800, Khyber Pakhtunkhwa, PakistanBiology Department, College of Science, Jouf University, Sakaka 2014, Saudi ArabiaBiology Department, Faculty of Science, Tabuk University, Tabuk 71491, Saudi ArabiaDepartment of Biology, College of Sciences and Humanities in AlKharj, Prince Sattam Bin Abdulaziz University, AlKharj 16278, Saudi ArabiaDepartment of Biology, College of Science, King Khalid University, Abha 61413, Saudi ArabiaDepartment of Biology, College of Science, King Khalid University, Abha 61413, Saudi ArabiaThe present study involved the bio-fabrication of silver nanoparticles (AgNPs) by using the <i>Euphorbia helioscopia</i> L. leaves aqueous extract to improve the production of secondary metabolites in industrially important sunflower (<i>Helianthus annuus</i> L.) plants. Phyto-fabrication of AgNPs was confirmed by using spectrophotometry, SEM imaging and X-ray diffraction analysis. The morphological and optical characterization manifested that the AgNPs are crystalline and exist in the size range of 30–100 nm. Various concentrations (10, 20, 40, 60, 80 and 100 mg/L) of AgNPs were applied in combinations on sunflower seeds and crop plants. The effects of biosynthesized AgNPs were evaluated for agro-morphological parameters (plant height, flowering initiation and seed weight), biochemical metabolites (chlorophyll, proline, soluble sugar, amino acid and protein contents) and enzymatic activities (superoxide dismutase and ascorbate peroxidase) in sunflower and 60 mg/L concentration of AgNPs on sunflower seeds and foliar sprays on plants in combination were found to be effective to elicit biochemical modifications to improve secondary metabolites. It was also observed experimentally that 60 mg/L concentration of AgNPs improved the biochemical, fatty acid and enzymatic attributes of sunflower plants, which in turn improved the plant agro-morphological parameters. Near-infrared spectroscopic analysis results confirmed the improvement in the seed quality, oil contents and fatty acid composition (palmitic acid, oleic acid and linoleic acid) after the applications of AgNPs. The findings of the present investigation confirm the exogenous applications of bio-fabricated AgNPs in combinations on seeds and plants to improve the plant yield, seed quality and secondary metabolite contents of the sunflower plants.https://www.mdpi.com/2079-4991/11/7/1750silver nanoparticlesagro-morphological parametersenzymatic activitiesbiochemical analysissecondary metabolites |
| spellingShingle | Syeda Umber Batool Bilal Javed Sohail Syeda Sadaf Zehra Zia-ur-Rehman Mashwani Naveed Iqbal Raja Tariq Khan Haifa Abdulaziz Sakit ALHaithloul Suliman Mohammed Alghanem Amina A. M. Al-Mushhin Mohamed Hashem Saad Alamri Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower Nanomaterials silver nanoparticles agro-morphological parameters enzymatic activities biochemical analysis secondary metabolites |
| title | Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower |
| title_full | Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower |
| title_fullStr | Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower |
| title_full_unstemmed | Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower |
| title_short | Exogenous Applications of Bio-fabricated Silver Nanoparticles to Improve Biochemical, Antioxidant, Fatty Acid and Secondary Metabolite Contents of Sunflower |
| title_sort | exogenous applications of bio fabricated silver nanoparticles to improve biochemical antioxidant fatty acid and secondary metabolite contents of sunflower |
| topic | silver nanoparticles agro-morphological parameters enzymatic activities biochemical analysis secondary metabolites |
| url | https://www.mdpi.com/2079-4991/11/7/1750 |
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