Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat
Objective-To evaluate effect of chitosan conduit for peripheral nerve regeneration using sciatic nerve transection model in rat<br /> Design- Experimental <em>in vivo</em> study.<br /> Animals- Sixty healthy male Wistar rats.<br /> Procedures-The rats were divided into...
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Iranian Veterinary Surgery Association (IVSA)
2010-08-01
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| Series: | Iranian Journal of Veterinary Surgery |
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| Online Access: | http://www.ivsajournals.com/article_3147_17a3df6ccb202dbc442715aca697a1c8.pdf |
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| author | Abbas Raisi Saeed Azizi Nowruz Delirezh Behnam Heshmatian Keyvan Amini |
| author_facet | Abbas Raisi Saeed Azizi Nowruz Delirezh Behnam Heshmatian Keyvan Amini |
| author_sort | Abbas Raisi |
| collection | DOAJ |
| description | Objective-To evaluate effect of chitosan conduit for peripheral nerve regeneration using sciatic nerve transection model in rat<br /> Design- Experimental <em>in vivo</em> study.<br /> Animals- Sixty healthy male Wistar rats.<br /> Procedures-The rats were divided into four experimental groups (n=15) randomly. In sham group the left sciatic nerve was exposed through a gluteal muscle incision and after careful homeostasis the wound was sutured. In transected control group the left sciatic nerve was exposed the same way, transected proximal to the tibio-peroneal bifurcation leaving a 10 mm gap and the nerve ends were sutured to the adjacent muscles. In silicone or chitosan groups the left sciatic nerve was transected the same way and proximal and distal stumps were each inserted into a silicone or chitosan tube. Each group was further subdivided into three subgroups of five animals each and were studied 4, 8, 12 weeks post operatively.<br /> Results- Functional and electrophysiological analyses showed significant improvement of nerve function in chitosan than in silicone group (<em>P < 0.05</em>). Morphometric indices and immuohistochemistry indicated that there were significant differences (<em>P < 0.05</em>) between chitosan and silicone with transected control groups 12 weeks after surgery.<br /> Conclusion and Clinical Relevance- Chitosan conduit could be considered clinically as an effective biodegradable tube for peripheral nerve regeneration in the least harmful way that is available, easily performed and affordable. It also averts the need for foreign materials that are likely to provoke a foreign body reaction. |
| first_indexed | 2024-12-11T20:44:44Z |
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| id | doaj.art-92114ea575e7408ca4f3737f279f3afc |
| institution | Directory Open Access Journal |
| issn | 2008-3033 2008-3033 |
| language | English |
| last_indexed | 2024-12-11T20:44:44Z |
| publishDate | 2010-08-01 |
| publisher | Iranian Veterinary Surgery Association (IVSA) |
| record_format | Article |
| series | Iranian Journal of Veterinary Surgery |
| spelling | doaj.art-92114ea575e7408ca4f3737f279f3afc2022-12-22T00:51:25ZengIranian Veterinary Surgery Association (IVSA)Iranian Journal of Veterinary Surgery2008-30332008-30332010-08-01051-2891003147Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of RatAbbas Raisi0Saeed Azizi1Nowruz Delirezh2Behnam Heshmatian3Keyvan Amini4Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Cellular and Molecular Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran.Neurophysiology research center, Urmia University of Medical Sciences, Urmia, Iran.Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon SK, Canada.Objective-To evaluate effect of chitosan conduit for peripheral nerve regeneration using sciatic nerve transection model in rat<br /> Design- Experimental <em>in vivo</em> study.<br /> Animals- Sixty healthy male Wistar rats.<br /> Procedures-The rats were divided into four experimental groups (n=15) randomly. In sham group the left sciatic nerve was exposed through a gluteal muscle incision and after careful homeostasis the wound was sutured. In transected control group the left sciatic nerve was exposed the same way, transected proximal to the tibio-peroneal bifurcation leaving a 10 mm gap and the nerve ends were sutured to the adjacent muscles. In silicone or chitosan groups the left sciatic nerve was transected the same way and proximal and distal stumps were each inserted into a silicone or chitosan tube. Each group was further subdivided into three subgroups of five animals each and were studied 4, 8, 12 weeks post operatively.<br /> Results- Functional and electrophysiological analyses showed significant improvement of nerve function in chitosan than in silicone group (<em>P < 0.05</em>). Morphometric indices and immuohistochemistry indicated that there were significant differences (<em>P < 0.05</em>) between chitosan and silicone with transected control groups 12 weeks after surgery.<br /> Conclusion and Clinical Relevance- Chitosan conduit could be considered clinically as an effective biodegradable tube for peripheral nerve regeneration in the least harmful way that is available, easily performed and affordable. It also averts the need for foreign materials that are likely to provoke a foreign body reaction.http://www.ivsajournals.com/article_3147_17a3df6ccb202dbc442715aca697a1c8.pdfPeripheral Nerve RegenerationChitosan conduitRat |
| spellingShingle | Abbas Raisi Saeed Azizi Nowruz Delirezh Behnam Heshmatian Keyvan Amini Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat Iranian Journal of Veterinary Surgery Peripheral Nerve Regeneration Chitosan conduit Rat |
| title | Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat |
| title_full | Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat |
| title_fullStr | Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat |
| title_full_unstemmed | Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat |
| title_short | Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat |
| title_sort | use of chitosan conduit for bridging small gap peripheral nerve defect in sciatic nerve transection model of rat |
| topic | Peripheral Nerve Regeneration Chitosan conduit Rat |
| url | http://www.ivsajournals.com/article_3147_17a3df6ccb202dbc442715aca697a1c8.pdf |
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