Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro
Abstract Staphylococcus pseudintermedius is an opportunistic pathogen causing a variety of infections that are difficult to treat, especially because of the development of antimicrobial resistance. It has a clonal distribution around the world. To have a better understanding of the MRSP population,...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47549-z |
| _version_ | 1797576711364149248 |
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| author | Izabel Mello Teixeira Yasmim de Moraes Assumpção Ana Clara Cabral Paletta Louise Aguiar Luciana Guimarães Isabella Thomaz da Silva Marina F. Côrtes Ana Maria Nunes Botelho Lauren Hubert Jaeger Renata Fernandes Ferreira Eliane de Oliveira Ferreira Bruno Penna |
| author_facet | Izabel Mello Teixeira Yasmim de Moraes Assumpção Ana Clara Cabral Paletta Louise Aguiar Luciana Guimarães Isabella Thomaz da Silva Marina F. Côrtes Ana Maria Nunes Botelho Lauren Hubert Jaeger Renata Fernandes Ferreira Eliane de Oliveira Ferreira Bruno Penna |
| author_sort | Izabel Mello Teixeira |
| collection | DOAJ |
| description | Abstract Staphylococcus pseudintermedius is an opportunistic pathogen causing a variety of infections that are difficult to treat, especially because of the development of antimicrobial resistance. It has a clonal distribution around the world. To have a better understanding of the MRSP population, we search the presence of MRSP in colonized or infected dogs. Samples from 99 dogs with infections and 35 from asymptomatic dogs were collected. Isolates were identified by mass spectrometry and Multiplex-PCR. The mecA gene was confirmed by conventional PCR. MRSP strains were analyzed by whole-genome sequencing. 75 S. pseudintermedius were identified, most from infection cases. The species were isolated from 70 out of the 135 dogs. Penicillin and Trimethoprim/Sulfamethoxazole presented higher resistance rates. Forty-seven strains were classified as multi-drug resistant (MDR), and were more isolated from dogs with infection (P < 0.05). Eighteen samples were classified as MRSP, representing 24.0% of the population. Six of 16 MRSP sequenced samples belonged to the world spread clone ST71; others belonged to unknown clones. Most samples carried the SCCmec type IIIA. Twenty-one different genetic resistance determinants were found among MRPS strains. MRSP is circulating among infected and colonized dogs in Rio de Janeiro, Brazil. |
| first_indexed | 2024-03-10T21:57:37Z |
| format | Article |
| id | doaj.art-9211f56e1f474f5796b08b276706d4c5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:57:37Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9211f56e1f474f5796b08b276706d4c52023-11-19T13:04:03ZengNature PortfolioScientific Reports2045-23222023-11-0113111010.1038/s41598-023-47549-zInvestigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de JaneiroIzabel Mello Teixeira0Yasmim de Moraes Assumpção1Ana Clara Cabral Paletta2Louise Aguiar3Luciana Guimarães4Isabella Thomaz da Silva5Marina F. Côrtes6Ana Maria Nunes Botelho7Lauren Hubert Jaeger8Renata Fernandes Ferreira9Eliane de Oliveira Ferreira10Bruno Penna11Laboratório de Biologia de Anaeróbios, Departamento Microbiologia Médica, IMPPG, UFRJLaboratório de Biologia de Anaeróbios, Departamento Microbiologia Médica, IMPPG, UFRJLaboratório de Cocos Gram Positivos, Departamento de Microbiologia e Parasitologia, UFFLaboratório de Cocos Gram Positivos, Departamento de Microbiologia e Parasitologia, UFFLaboratório de Cocos Gram Positivos, Departamento de Microbiologia e Parasitologia, UFFLaboratório de Cocos Gram Positivos, Departamento de Microbiologia e Parasitologia, UFFLaboratório de Investigação Médica, Instituto de Medicina Tropical, USPLaboratório de Cocos Gram Positivos, Departamento de Microbiologia e Parasitologia, UFFLaboratório de Células-Tronco e Parasitologia Molecular, Departamento de Ciências Farmacêuticas, UFJFUniversidade de VassourasLaboratório de Biologia de Anaeróbios, Departamento Microbiologia Médica, IMPPG, UFRJLaboratório de Cocos Gram Positivos, Departamento de Microbiologia e Parasitologia, UFFAbstract Staphylococcus pseudintermedius is an opportunistic pathogen causing a variety of infections that are difficult to treat, especially because of the development of antimicrobial resistance. It has a clonal distribution around the world. To have a better understanding of the MRSP population, we search the presence of MRSP in colonized or infected dogs. Samples from 99 dogs with infections and 35 from asymptomatic dogs were collected. Isolates were identified by mass spectrometry and Multiplex-PCR. The mecA gene was confirmed by conventional PCR. MRSP strains were analyzed by whole-genome sequencing. 75 S. pseudintermedius were identified, most from infection cases. The species were isolated from 70 out of the 135 dogs. Penicillin and Trimethoprim/Sulfamethoxazole presented higher resistance rates. Forty-seven strains were classified as multi-drug resistant (MDR), and were more isolated from dogs with infection (P < 0.05). Eighteen samples were classified as MRSP, representing 24.0% of the population. Six of 16 MRSP sequenced samples belonged to the world spread clone ST71; others belonged to unknown clones. Most samples carried the SCCmec type IIIA. Twenty-one different genetic resistance determinants were found among MRPS strains. MRSP is circulating among infected and colonized dogs in Rio de Janeiro, Brazil.https://doi.org/10.1038/s41598-023-47549-z |
| spellingShingle | Izabel Mello Teixeira Yasmim de Moraes Assumpção Ana Clara Cabral Paletta Louise Aguiar Luciana Guimarães Isabella Thomaz da Silva Marina F. Côrtes Ana Maria Nunes Botelho Lauren Hubert Jaeger Renata Fernandes Ferreira Eliane de Oliveira Ferreira Bruno Penna Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro Scientific Reports |
| title | Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro |
| title_full | Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro |
| title_fullStr | Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro |
| title_full_unstemmed | Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro |
| title_short | Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro |
| title_sort | investigation of antimicrobial susceptibility and genetic diversity among staphylococcus pseudintermedius isolated from dogs in rio de janeiro |
| url | https://doi.org/10.1038/s41598-023-47549-z |
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