Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection
The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-10-01
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| Series: | Biosensors |
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| Online Access: | http://www.mdpi.com/2079-6374/8/4/91 |
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| author | Francesco Milano Livia Giotta Daniela Chirizzi Simos Papazoglou Christina Kryou Annarita De Bartolomeo Vincenzo De Leo Maria Rachele Guascito Ioanna Zergioti |
| author_facet | Francesco Milano Livia Giotta Daniela Chirizzi Simos Papazoglou Christina Kryou Annarita De Bartolomeo Vincenzo De Leo Maria Rachele Guascito Ioanna Zergioti |
| author_sort | Francesco Milano |
| collection | DOAJ |
| description | The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition of various materials (even labile materials like enzymes) that results in intimate and stable contact with target surface. In this work, Pt, Au, and glassy carbon screen printed electrodes (SPEs) treated by LIFT with phosphate buffer have been characterized by scanning electron microscopy and atomic force microscopy to reveal a flattening effect of all surfaces. The electrochemical characterization by cyclic voltammetry shows significant differences depending on the electrode material. The electroactivity of Au is reduced while that of glassy carbon and Pt is greatly enhanced. In particular, the electrochemical behavior of a phosphate LIFT treated Pt showed a marked enrichment of hydrogen adsorbed layer, suggesting an elevated electrocatalytic activity towards glucose oxidation. When Pt electrodes modified in this way were used as an effective glucose sensor, a 1–10 mM linear response and a 10 µM detection limit were obtained. A possible role of phosphate that was securely immobilized on a Pt surface, as evidenced by XPS analysis, enhancing the glucose electrooxidation is discussed. |
| first_indexed | 2024-04-13T03:06:42Z |
| format | Article |
| id | doaj.art-92153dbb0e6f45e18f3b997b590beae0 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-04-13T03:06:42Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-92153dbb0e6f45e18f3b997b590beae02022-12-22T03:05:13ZengMDPI AGBiosensors2079-63742018-10-01849110.3390/bios8040091bios8040091Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose DetectionFrancesco Milano0Livia Giotta1Daniela Chirizzi2Simos Papazoglou3Christina Kryou4Annarita De Bartolomeo5Vincenzo De Leo6Maria Rachele Guascito7Ioanna Zergioti8Istituto per i Processi Chimico Fisici, UOS Bari, Via Orabona 4, 70126 Bari, ItalyDipartimento di Scienze e Tecnologie Biologiche e Ambientali, Università del Salento, S.P. Lecce-Monteroni, 73100 Lecce, ItalyIZS Puglia e Basilicata, U.O. Putignano. Via Chiancolla 1, C.da. S. Pietro Piturno, 70017 Putignano (BA), ItalyDepartment of Physics, National Technical University of Athens, Iroon Polytehneiou 9, Zografou, 15780 Athens, GreeceDepartment of Physics, National Technical University of Athens, Iroon Polytehneiou 9, Zografou, 15780 Athens, GreeceDipartimento di Beni Culturali, Università del Salento, S.P. Lecce-Monteroni, 73100 Lecce, ItalyIstituto per i Processi Chimico Fisici, UOS Bari, Via Orabona 4, 70126 Bari, ItalyDipartimento di Scienze e Tecnologie Biologiche e Ambientali, Università del Salento, S.P. Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Physics, National Technical University of Athens, Iroon Polytehneiou 9, Zografou, 15780 Athens, GreeceThe design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition of various materials (even labile materials like enzymes) that results in intimate and stable contact with target surface. In this work, Pt, Au, and glassy carbon screen printed electrodes (SPEs) treated by LIFT with phosphate buffer have been characterized by scanning electron microscopy and atomic force microscopy to reveal a flattening effect of all surfaces. The electrochemical characterization by cyclic voltammetry shows significant differences depending on the electrode material. The electroactivity of Au is reduced while that of glassy carbon and Pt is greatly enhanced. In particular, the electrochemical behavior of a phosphate LIFT treated Pt showed a marked enrichment of hydrogen adsorbed layer, suggesting an elevated electrocatalytic activity towards glucose oxidation. When Pt electrodes modified in this way were used as an effective glucose sensor, a 1–10 mM linear response and a 10 µM detection limit were obtained. A possible role of phosphate that was securely immobilized on a Pt surface, as evidenced by XPS analysis, enhancing the glucose electrooxidation is discussed.http://www.mdpi.com/2079-6374/8/4/91screen printed electrodeslaser printingLIFTglucosebiosensor |
| spellingShingle | Francesco Milano Livia Giotta Daniela Chirizzi Simos Papazoglou Christina Kryou Annarita De Bartolomeo Vincenzo De Leo Maria Rachele Guascito Ioanna Zergioti Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection Biosensors screen printed electrodes laser printing LIFT glucose biosensor |
| title | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
| title_full | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
| title_fullStr | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
| title_full_unstemmed | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
| title_short | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
| title_sort | phosphate modified screen printed electrodes by lift treatment for glucose detection |
| topic | screen printed electrodes laser printing LIFT glucose biosensor |
| url | http://www.mdpi.com/2079-6374/8/4/91 |
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