Effects of Short-Term Intermittent Fasting on Growth Performance, Fatty Acids Profile, Glycolysis and Cholesterol Synthesis Gene Expression in European Seabass <i>Dicentrarchus labrax</i>
The present study was applied to evaluate the effects of alternate feeding and feed restriction on gene expression, growth, proximate composition and biochemical indices in European seabass, <i>Dicentrarchus labrax</i>. Fish were randomly divided into six indoor tanks with 90 fish per ta...
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MDPI AG
2023-11-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/8/12/582 |
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| author | Olga Ntantali Emmanouil E. Malandrakis Wout Abbink John Bastiaansen Evanthia Chatzoglou Ioannis T. Karapanagiotidis Eleni Golomazou Panagiota Panagiotaki |
| author_facet | Olga Ntantali Emmanouil E. Malandrakis Wout Abbink John Bastiaansen Evanthia Chatzoglou Ioannis T. Karapanagiotidis Eleni Golomazou Panagiota Panagiotaki |
| author_sort | Olga Ntantali |
| collection | DOAJ |
| description | The present study was applied to evaluate the effects of alternate feeding and feed restriction on gene expression, growth, proximate composition and biochemical indices in European seabass, <i>Dicentrarchus labrax</i>. Fish were randomly divided into six indoor tanks with 90 fish per tank in a recirculating aquaculture system. Two feeding strategies were applied, in which the first group was fed daily to satiation and the second was intermittently fed (8 days feeding to satiation–2 days starvation) for 40 days. At the end of the experiment, outlier fish were sorted as fast growers (FG) and slow growers (SG) according to their final body weight. The differential gene expression tested was related to glycolysis (<i>pk</i>, <i>ldha</i>, <i>hk</i>, <i>g3pdh</i>, <i>eno1</i> and <i>alda</i>), fatty acid metabolism (<i>lpl</i> and <i>acc</i>) and cholesterol synthesis (<i>7dhcr</i> and <i>sqle</i>). In addition, muscle <i>ldha</i> and <i>gpi</i> expressions were positively correlated with fish weight. The concentrations of glucose, triglycerides, cholesterol and non-esterified fatty acids (NEFA) were not affected by the dietary treatments. Glucose and NEFA differed significantly between SG and FG fed groups. Overall, the physiological responses of glucose and fatty acid metabolism in fish, as recorded by gene expression assays, were triggered by minor interventions in feeding rather than the different growth rates. Expression of specific genes and biochemical parameters could be used as potential biomarkers to improve aquaculture practices and benefit fish husbandry through selective breeding, feeding strategies and farm management. The study provides new insights on the impact of intermittent feeding of European seabass, with gene markers and their potential effects, for European seabass aquaculture. |
| first_indexed | 2024-03-08T20:46:57Z |
| format | Article |
| id | doaj.art-92192e4213394eb6b228615c1acb721b |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-08T20:46:57Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-92192e4213394eb6b228615c1acb721b2023-12-22T14:08:12ZengMDPI AGFishes2410-38882023-11-0181258210.3390/fishes8120582Effects of Short-Term Intermittent Fasting on Growth Performance, Fatty Acids Profile, Glycolysis and Cholesterol Synthesis Gene Expression in European Seabass <i>Dicentrarchus labrax</i>Olga Ntantali0Emmanouil E. Malandrakis1Wout Abbink2John Bastiaansen3Evanthia Chatzoglou4Ioannis T. Karapanagiotidis5Eleni Golomazou6Panagiota Panagiotaki7Laboratory of Aquaculture, Department of Ichthyology and Aquatic Environment, University of Thessaly, Fitokou Street, 38446 Volos, GreeceLaboratory of Applied Hydrobiology, Department of Animal Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceAnimal Breeding & Genomics, Wageningen Livestock Research, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The NetherlandsAnimal Breeding & Genomics, Wageningen Livestock Research, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The NetherlandsLaboratory of Applied Hydrobiology, Department of Animal Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceLaboratory of Aquaculture, Department of Ichthyology and Aquatic Environment, University of Thessaly, Fitokou Street, 38446 Volos, GreeceLaboratory of Aquaculture, Department of Ichthyology and Aquatic Environment, University of Thessaly, Fitokou Street, 38446 Volos, GreeceLaboratory of Aquaculture, Department of Ichthyology and Aquatic Environment, University of Thessaly, Fitokou Street, 38446 Volos, GreeceThe present study was applied to evaluate the effects of alternate feeding and feed restriction on gene expression, growth, proximate composition and biochemical indices in European seabass, <i>Dicentrarchus labrax</i>. Fish were randomly divided into six indoor tanks with 90 fish per tank in a recirculating aquaculture system. Two feeding strategies were applied, in which the first group was fed daily to satiation and the second was intermittently fed (8 days feeding to satiation–2 days starvation) for 40 days. At the end of the experiment, outlier fish were sorted as fast growers (FG) and slow growers (SG) according to their final body weight. The differential gene expression tested was related to glycolysis (<i>pk</i>, <i>ldha</i>, <i>hk</i>, <i>g3pdh</i>, <i>eno1</i> and <i>alda</i>), fatty acid metabolism (<i>lpl</i> and <i>acc</i>) and cholesterol synthesis (<i>7dhcr</i> and <i>sqle</i>). In addition, muscle <i>ldha</i> and <i>gpi</i> expressions were positively correlated with fish weight. The concentrations of glucose, triglycerides, cholesterol and non-esterified fatty acids (NEFA) were not affected by the dietary treatments. Glucose and NEFA differed significantly between SG and FG fed groups. Overall, the physiological responses of glucose and fatty acid metabolism in fish, as recorded by gene expression assays, were triggered by minor interventions in feeding rather than the different growth rates. Expression of specific genes and biochemical parameters could be used as potential biomarkers to improve aquaculture practices and benefit fish husbandry through selective breeding, feeding strategies and farm management. The study provides new insights on the impact of intermittent feeding of European seabass, with gene markers and their potential effects, for European seabass aquaculture.https://www.mdpi.com/2410-3888/8/12/582<i>Dicentrarchus labrax</i>intermittent feedinggene expressiongrowthbody compositionbiochemical indices |
| spellingShingle | Olga Ntantali Emmanouil E. Malandrakis Wout Abbink John Bastiaansen Evanthia Chatzoglou Ioannis T. Karapanagiotidis Eleni Golomazou Panagiota Panagiotaki Effects of Short-Term Intermittent Fasting on Growth Performance, Fatty Acids Profile, Glycolysis and Cholesterol Synthesis Gene Expression in European Seabass <i>Dicentrarchus labrax</i> Fishes <i>Dicentrarchus labrax</i> intermittent feeding gene expression growth body composition biochemical indices |
| title | Effects of Short-Term Intermittent Fasting on Growth Performance, Fatty Acids Profile, Glycolysis and Cholesterol Synthesis Gene Expression in European Seabass <i>Dicentrarchus labrax</i> |
| title_full | Effects of Short-Term Intermittent Fasting on Growth Performance, Fatty Acids Profile, Glycolysis and Cholesterol Synthesis Gene Expression in European Seabass <i>Dicentrarchus labrax</i> |
| title_fullStr | Effects of Short-Term Intermittent Fasting on Growth Performance, Fatty Acids Profile, Glycolysis and Cholesterol Synthesis Gene Expression in European Seabass <i>Dicentrarchus labrax</i> |
| title_full_unstemmed | Effects of Short-Term Intermittent Fasting on Growth Performance, Fatty Acids Profile, Glycolysis and Cholesterol Synthesis Gene Expression in European Seabass <i>Dicentrarchus labrax</i> |
| title_short | Effects of Short-Term Intermittent Fasting on Growth Performance, Fatty Acids Profile, Glycolysis and Cholesterol Synthesis Gene Expression in European Seabass <i>Dicentrarchus labrax</i> |
| title_sort | effects of short term intermittent fasting on growth performance fatty acids profile glycolysis and cholesterol synthesis gene expression in european seabass i dicentrarchus labrax i |
| topic | <i>Dicentrarchus labrax</i> intermittent feeding gene expression growth body composition biochemical indices |
| url | https://www.mdpi.com/2410-3888/8/12/582 |
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