Plasma-derived exosomes contributes to endothelial-to-mesenchymal transition in Moyamoya disease
Background: Moyamoya disease (MMD) is a cerebrovascular disease with a high disability rate; however, its pathogenesis remains unknown. Endothelial-mesenchymal transition (EndMT) is the pathological basis of many vascular diseases; however, the key role of EndMT in MMD has not yet been reported. Met...
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| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024027798 |
| _version_ | 1797259875207610368 |
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| author | Jilan Liu Chao Chen Xianyun Qin Jingtong Wang Bin Zhang Feng Jin |
| author_facet | Jilan Liu Chao Chen Xianyun Qin Jingtong Wang Bin Zhang Feng Jin |
| author_sort | Jilan Liu |
| collection | DOAJ |
| description | Background: Moyamoya disease (MMD) is a cerebrovascular disease with a high disability rate; however, its pathogenesis remains unknown. Endothelial-mesenchymal transition (EndMT) is the pathological basis of many vascular diseases; however, the key role of EndMT in MMD has not yet been reported. Method: We collected vascular tissues from three control samples and six patients with MMD to detect the expression of EndMT-related genes. To elucidate the mechanism of EndMT in MMD, we performed in vitro cell experiments. Plasma-derived exosomes (PDEs) can transmit information between cells and tissues and are of considerable importance in several disease studies. PDEs were used to stimulate EndMT phenotype in cerebrovascular endothelial cells. Results: Multiplex fluorescent immunohistochemistry staining confirmed that CD31, VE-cadherin and E-cadherin down-regulated, whereas α-SMA and vimentin were significantly up-regulated in moyamoya vascular endothelial cells than in control samples. PDEs from MMD patients significantly promoted cell proliferation and migration, resulting in slender cells. PDEs induce EndMT-related phenotype changes in cerebral vascular endothelial cells, including decreased endothelial cell marker expression and increased mesenchymal cell marker expression. We demonstrated that EndMT phenotypic alterations are mediated, in part, by microRNA(miRNAs). Conclusion: This study was the first to propose that EndMT may exist in the vessels of patients with MMD. PDEs induce the EndMT phenotype to promote the development of MMD. This study aimed to provide a new theoretical basis for elucidating the pathogenesis of MMD. |
| first_indexed | 2024-03-07T20:04:16Z |
| format | Article |
| id | doaj.art-921be50d951444b8be4d47d120b8d44d |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:16:22Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-921be50d951444b8be4d47d120b8d44d2024-03-17T07:56:09ZengElsevierHeliyon2405-84402024-03-01105e26748Plasma-derived exosomes contributes to endothelial-to-mesenchymal transition in Moyamoya diseaseJilan Liu0Chao Chen1Xianyun Qin2Jingtong Wang3Bin Zhang4Feng Jin5Department of Medical Research Center, Affiliated Hospital of Jining Medical University, Jining, Shandong, 272029, PR ChinaDepartment of Neurosurgery, Qingdao Central Hospital, University of Health and Rehabilitation Sciences ＆Qingdao Central Hospital Medical Group, Qingdao, Shandong, 266042, PR ChinaDepartment of Medical Research Center, Affiliated Hospital of Jining Medical University, Jining, Shandong, 272029, PR ChinaDepartment of Emergency Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong, 272029, PR ChinaDepartment of Laboratory Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong, 272029, PR ChinaDepartment of Neurosurgery, Qingdao Central Hospital, University of Health and Rehabilitation Sciences ＆Qingdao Central Hospital Medical Group, Qingdao, Shandong, 266042, PR China; Corresponding author.Background: Moyamoya disease (MMD) is a cerebrovascular disease with a high disability rate; however, its pathogenesis remains unknown. Endothelial-mesenchymal transition (EndMT) is the pathological basis of many vascular diseases; however, the key role of EndMT in MMD has not yet been reported. Method: We collected vascular tissues from three control samples and six patients with MMD to detect the expression of EndMT-related genes. To elucidate the mechanism of EndMT in MMD, we performed in vitro cell experiments. Plasma-derived exosomes (PDEs) can transmit information between cells and tissues and are of considerable importance in several disease studies. PDEs were used to stimulate EndMT phenotype in cerebrovascular endothelial cells. Results: Multiplex fluorescent immunohistochemistry staining confirmed that CD31, VE-cadherin and E-cadherin down-regulated, whereas α-SMA and vimentin were significantly up-regulated in moyamoya vascular endothelial cells than in control samples. PDEs from MMD patients significantly promoted cell proliferation and migration, resulting in slender cells. PDEs induce EndMT-related phenotype changes in cerebral vascular endothelial cells, including decreased endothelial cell marker expression and increased mesenchymal cell marker expression. We demonstrated that EndMT phenotypic alterations are mediated, in part, by microRNA(miRNAs). Conclusion: This study was the first to propose that EndMT may exist in the vessels of patients with MMD. PDEs induce the EndMT phenotype to promote the development of MMD. This study aimed to provide a new theoretical basis for elucidating the pathogenesis of MMD.http://www.sciencedirect.com/science/article/pii/S2405844024027798Moyamoya diseasePlasmaExosomesEndothelial-to-mesenchymal transitionmicroRNA |
| spellingShingle | Jilan Liu Chao Chen Xianyun Qin Jingtong Wang Bin Zhang Feng Jin Plasma-derived exosomes contributes to endothelial-to-mesenchymal transition in Moyamoya disease Heliyon Moyamoya disease Plasma Exosomes Endothelial-to-mesenchymal transition microRNA |
| title | Plasma-derived exosomes contributes to endothelial-to-mesenchymal transition in Moyamoya disease |
| title_full | Plasma-derived exosomes contributes to endothelial-to-mesenchymal transition in Moyamoya disease |
| title_fullStr | Plasma-derived exosomes contributes to endothelial-to-mesenchymal transition in Moyamoya disease |
| title_full_unstemmed | Plasma-derived exosomes contributes to endothelial-to-mesenchymal transition in Moyamoya disease |
| title_short | Plasma-derived exosomes contributes to endothelial-to-mesenchymal transition in Moyamoya disease |
| title_sort | plasma derived exosomes contributes to endothelial to mesenchymal transition in moyamoya disease |
| topic | Moyamoya disease Plasma Exosomes Endothelial-to-mesenchymal transition microRNA |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024027798 |
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