Enchytraeus albidus microarray: enrichment, design, annotation and database (EnchyBASE).
Enchytraeus albidus (Oligochaeta) is an ecologically relevant species used as standard test organisms for risk assessment. Effects of stressors in this species are commonly determined at the population level using reproduction and survival as endpoints. The assessment of transcriptomic responses can...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3338728?pdf=render |
| _version_ | 1818906307422322688 |
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| author | Sara C Novais Joel Arrais Pedro Lopes Tine Vandenbrouck Wim De Coen Dick Roelofs Amadeu M V M Soares Mónica J B Amorim |
| author_facet | Sara C Novais Joel Arrais Pedro Lopes Tine Vandenbrouck Wim De Coen Dick Roelofs Amadeu M V M Soares Mónica J B Amorim |
| author_sort | Sara C Novais |
| collection | DOAJ |
| description | Enchytraeus albidus (Oligochaeta) is an ecologically relevant species used as standard test organisms for risk assessment. Effects of stressors in this species are commonly determined at the population level using reproduction and survival as endpoints. The assessment of transcriptomic responses can be very useful e.g. to understand underlying mechanisms of toxicity with gene expression fingerprinting. In the present paper the following is being addressed: 1) development of suppressive subtractive hybridization (SSH) libraries enriched for differentially expressed genes after metal and pesticide exposures; 2) sequencing and characterization of all generated cDNA inserts; 3) development of a publicly available genomic database on E. albidus. A total of 2100 Expressed Sequence Tags (ESTs) were isolated, sequenced and assembled into 1124 clusters (947 singletons and 177 contigs). From these sequences, 41% matched known proteins in GenBank (BLASTX, e-value ≤ 10(-5)) and 37% had at least one Gene Ontology (GO) term assigned. In total, 5.5% of the sequences were assigned to a metabolic pathway, based on KEGG. With this new sequencing information, an Agilent custom oligonucleotide microarray was designed, representing a potential tool for transcriptomic studies. EnchyBASE (http://bioinformatics.ua.pt/enchybase/) was developed as a web freely available database containing genomic information on E. albidus and will be further extended in the near future for other enchytraeid species. The database so far includes all ESTs generated for E. albidus from three cDNA libraries. This information can be downloaded and applied in functional genomics and transcription studies. |
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| format | Article |
| id | doaj.art-921d3a15bc744a2d9476f2af49853fd2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T21:37:09Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-921d3a15bc744a2d9476f2af49853fd22022-12-21T20:04:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3426610.1371/journal.pone.0034266Enchytraeus albidus microarray: enrichment, design, annotation and database (EnchyBASE).Sara C NovaisJoel ArraisPedro LopesTine VandenbrouckWim De CoenDick RoelofsAmadeu M V M SoaresMónica J B AmorimEnchytraeus albidus (Oligochaeta) is an ecologically relevant species used as standard test organisms for risk assessment. Effects of stressors in this species are commonly determined at the population level using reproduction and survival as endpoints. The assessment of transcriptomic responses can be very useful e.g. to understand underlying mechanisms of toxicity with gene expression fingerprinting. In the present paper the following is being addressed: 1) development of suppressive subtractive hybridization (SSH) libraries enriched for differentially expressed genes after metal and pesticide exposures; 2) sequencing and characterization of all generated cDNA inserts; 3) development of a publicly available genomic database on E. albidus. A total of 2100 Expressed Sequence Tags (ESTs) were isolated, sequenced and assembled into 1124 clusters (947 singletons and 177 contigs). From these sequences, 41% matched known proteins in GenBank (BLASTX, e-value ≤ 10(-5)) and 37% had at least one Gene Ontology (GO) term assigned. In total, 5.5% of the sequences were assigned to a metabolic pathway, based on KEGG. With this new sequencing information, an Agilent custom oligonucleotide microarray was designed, representing a potential tool for transcriptomic studies. EnchyBASE (http://bioinformatics.ua.pt/enchybase/) was developed as a web freely available database containing genomic information on E. albidus and will be further extended in the near future for other enchytraeid species. The database so far includes all ESTs generated for E. albidus from three cDNA libraries. This information can be downloaded and applied in functional genomics and transcription studies.http://europepmc.org/articles/PMC3338728?pdf=render |
| spellingShingle | Sara C Novais Joel Arrais Pedro Lopes Tine Vandenbrouck Wim De Coen Dick Roelofs Amadeu M V M Soares Mónica J B Amorim Enchytraeus albidus microarray: enrichment, design, annotation and database (EnchyBASE). PLoS ONE |
| title | Enchytraeus albidus microarray: enrichment, design, annotation and database (EnchyBASE). |
| title_full | Enchytraeus albidus microarray: enrichment, design, annotation and database (EnchyBASE). |
| title_fullStr | Enchytraeus albidus microarray: enrichment, design, annotation and database (EnchyBASE). |
| title_full_unstemmed | Enchytraeus albidus microarray: enrichment, design, annotation and database (EnchyBASE). |
| title_short | Enchytraeus albidus microarray: enrichment, design, annotation and database (EnchyBASE). |
| title_sort | enchytraeus albidus microarray enrichment design annotation and database enchybase |
| url | http://europepmc.org/articles/PMC3338728?pdf=render |
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