Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Abstract Background Bacteria of the genus Photorhabdus and Xenorhabdus are motile, Gram-negative bacteria that live in symbiosis with entomopathogenic nematodes. Due to their complex life cycle, they produce a large number of specialized metabolites (natural products) encoded in biosynthetic gene cl...

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Bibliographic Details
Main Authors: Alexander Rill, Lei Zhao, Helge B. Bode
Format: Article
Language:English
Published: BMC 2024-04-01
Series:Microbial Cell Factories
Subjects:
CRISPR/Cpf1
Xenorhabdus
Photorhabdus
Genome editing
Safracin
Natural products
Online Access:https://doi.org/10.1186/s12934-024-02363-8

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