Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins
Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing an...
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| Formato: | Artículo |
| Lenguaje: | English |
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2018-12-01
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| Colección: | Polish Journal of Food and Nutrition Sciences |
| Materias: | |
| Acceso en línea: | http://www.degruyter.com/view/j/pjfns.2018.68.issue-4/pjfns-2018-000b/pjfns-2018-000b.xml?format=INT |
| _version_ | 1826830174668193792 |
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| author | Joehnke Marcel Skejovic Sørensen Susanne Bjergegaard Charlotte Markedal Keld Ejdrup Sørensen Jens Christian |
| author_facet | Joehnke Marcel Skejovic Sørensen Susanne Bjergegaard Charlotte Markedal Keld Ejdrup Sørensen Jens Christian |
| author_sort | Joehnke Marcel Skejovic |
| collection | DOAJ |
| description | Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level. |
| first_indexed | 2024-04-12T04:27:50Z |
| format | Article |
| id | doaj.art-921f1121938745afacad06a79960f16d |
| institution | Directory Open Access Journal |
| issn | 2083-6007 |
| language | English |
| last_indexed | 2025-02-16T09:30:44Z |
| publishDate | 2018-12-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj.art-921f1121938745afacad06a79960f16d2025-02-03T02:36:55ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072018-12-0168433534510.2478/pjfns-2018-0005pjfns-2018-000bEffect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin ProteinsJoehnke Marcel Skejovic0Sørensen Susanne1Bjergegaard Charlotte2Markedal Keld Ejdrup3Sørensen Jens Christian4Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958Frederiksberg C, DenmarkDepartment of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958Frederiksberg C, DenmarkDepartment of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958Frederiksberg C, DenmarkDepartment of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958Frederiksberg C, DenmarkDepartment of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958Frederiksberg C, DenmarkProtein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.http://www.degruyter.com/view/j/pjfns.2018.68.issue-4/pjfns-2018-000b/pjfns-2018-000b.xml?format=INTin vitroprotein digestibilitytotal dietary fibrerapeseed napin proteinsplant food ingredients |
| spellingShingle | Joehnke Marcel Skejovic Sørensen Susanne Bjergegaard Charlotte Markedal Keld Ejdrup Sørensen Jens Christian Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins Polish Journal of Food and Nutrition Sciences in vitro protein digestibility total dietary fibre rapeseed napin proteins plant food ingredients |
| title | Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins |
| title_full | Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins |
| title_fullStr | Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins |
| title_full_unstemmed | Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins |
| title_short | Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins |
| title_sort | effect of dietary fibre fractions on in vitro digestibility of rapeseed napin proteins |
| topic | in vitro protein digestibility total dietary fibre rapeseed napin proteins plant food ingredients |
| url | http://www.degruyter.com/view/j/pjfns.2018.68.issue-4/pjfns-2018-000b/pjfns-2018-000b.xml?format=INT |
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