Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks
Objective: To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks. Methods: Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10-day intervals for...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.apjr.net/article.asp?issn=2305-0500;year=2021;volume=10;issue=4;spage=187;epage=194;aulast=Lukusa |
| _version_ | 1818649712957325312 |
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| author | Kambulu Lukusa John Kabuba |
| author_facet | Kambulu Lukusa John Kabuba |
| author_sort | Kambulu Lukusa |
| collection | DOAJ |
| description | Objective: To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks.
Methods: Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10-day intervals for three months. Sperm kinematic parameters, morphological parameters, mitochondrial membrane potential, plasma membrane functionality, and sperm DNA integrity were evaluated weekly pre and post-cryopreservation.
Results: The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method (P<0.05). Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation (P<0.05). Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups (P<0.05). The sperm kinematic parameters such as curvilinear velocity, average path velocity, and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05). The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups (P<0.05). In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups, respectively (P<0.05).
Conclusions: Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck. |
| first_indexed | 2024-12-17T01:38:41Z |
| format | Article |
| id | doaj.art-922534f6ab3f4967b9cafbd144a24c96 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 2305-0519 |
| language | English |
| last_indexed | 2024-12-17T01:38:41Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-922534f6ab3f4967b9cafbd144a24c962022-12-21T22:08:22ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002305-05192021-01-0110418719410.4103/2305-0500.321126Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucksKambulu LukusaJohn KabubaObjective: To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks. Methods: Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10-day intervals for three months. Sperm kinematic parameters, morphological parameters, mitochondrial membrane potential, plasma membrane functionality, and sperm DNA integrity were evaluated weekly pre and post-cryopreservation. Results: The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method (P<0.05). Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation (P<0.05). Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups (P<0.05). The sperm kinematic parameters such as curvilinear velocity, average path velocity, and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05). The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups (P<0.05). In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups, respectively (P<0.05). Conclusions: Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.http://www.apjr.net/article.asp?issn=2305-0500;year=2021;volume=10;issue=4;spage=187;epage=194;aulast=Lukusapost thaw; cryopreservation; saanen buck; selenium supplementation; semen collection method; sperm kinematic parameters; sperm motility; sperm quality |
| spellingShingle | Kambulu Lukusa John Kabuba Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks Asian Pacific Journal of Reproduction post thaw; cryopreservation; saanen buck; selenium supplementation; semen collection method; sperm kinematic parameters; sperm motility; sperm quality |
| title | Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks |
| title_full | Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks |
| title_fullStr | Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks |
| title_full_unstemmed | Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks |
| title_short | Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks |
| title_sort | oral supplementation of selenium improves post thaw sperm quality in saanen bucks |
| topic | post thaw; cryopreservation; saanen buck; selenium supplementation; semen collection method; sperm kinematic parameters; sperm motility; sperm quality |
| url | http://www.apjr.net/article.asp?issn=2305-0500;year=2021;volume=10;issue=4;spage=187;epage=194;aulast=Lukusa |
| work_keys_str_mv | AT kambululukusa oralsupplementationofseleniumimprovespostthawspermqualityinsaanenbucks AT johnkabuba oralsupplementationofseleniumimprovespostthawspermqualityinsaanenbucks |