Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler model
ABSTRACT: In order to study the prevention and control EHEC disease measures in poultry, the infection process and development of this disease and the pathological changes of various organs were to be observed. In this study, chickens were infected with different doses of enterohemorrhagic Escherich...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001408 |
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| author | Yan-Na Guo Rui-Wei Mou Meng-Han Lu Shao-Shan Liang Yong-Ming He Lu-Ping Tang |
| author_facet | Yan-Na Guo Rui-Wei Mou Meng-Han Lu Shao-Shan Liang Yong-Ming He Lu-Ping Tang |
| author_sort | Yan-Na Guo |
| collection | DOAJ |
| description | ABSTRACT: In order to study the prevention and control EHEC disease measures in poultry, the infection process and development of this disease and the pathological changes of various organs were to be observed. In this study, chickens were infected with different doses of enterohemorrhagic Escherichia coli (EHEC) O157:H7 using different routes of administration to establish EHEC broiler model. A total of 195 14-day-old broilers were randomly divided into 13 groups: including control group, Enema-drip groups (1010, 1011, 1012, 1013 CFUs E. coli O157:H7), gavage groups (P.O) (1011, 1012, 1013, 1014 CFUs E. coli O157:H7), and intraperitoneal injection group (I.P.) (108, 109, 1010, 1011 CFUs E. coli O157:H7). Escherichia coli (E. coli) was given using enema-drip, gavage or intraperitoneal infection. Then the feed intake, weight changes, stool and clinical symptoms of the chicks were recorded during the experiment. 7 d after E. coli infection, blood was collected from the jugular vein and serological tests were carried out. The liver, spleen, and colon of the chicks were extracted to get the organ index, bacteria load, and their histopathological changes. After infection with E. coli, some chicks feces were green or red watery stool, sometimes accompanied by foam, and the material to weight ratio of broilers in I.P. group increased significantly (P < 0.05), the 108 CFUs group were 1.3 times as large as control group. Three modeling methods can result in abnormal serum lipid metabolism and liver function indexes (increase of AST, TBA, T-Bil and TC level; decrease of ALB, TG, and TP level). Infection of chicks with O157:H7 by all 3 methods resulted in its detection in the liver, spleen, and colon. Three modeling methods significantly decreased liver index, and inflammatory cell infiltration and hyperemia were observed in liver. The spleen index in E. coli broilers by gavage and enema-drip was significantly decreased, splenic hyperemia and periarteriolar hyalinosis were observed. The spleen was enlarged with purplish-black spheroids in I.P. group broilers, and the spleen histological changes was more serious. The colon villi of broilers in gavage and enema-drip groups were thinner, more prone to rupture, intestinal lamina propria hyperemia, and inflammatory cell infiltration. Moreover, the number of goblet cells in the mucosal epithelium increased. E. coli O157:H7 can induce liver, spleen and intestinal damage and reduce growth performance of chicks. By comparing these 3 methods, we found that chicks infected with O157:H7 by gavage had more severe liver and intestinal damage, the enema-drip method caused most serious intestinal damage, and I.P. method significantly damaged the liver and spleen of chickens. |
| first_indexed | 2024-03-07T20:06:12Z |
| format | Article |
| id | doaj.art-922758ae052d4e569434064d643284d8 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T22:22:36Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj.art-922758ae052d4e569434064d643284d82024-03-20T06:08:05ZengElsevierPoultry Science0032-57912024-04-011034103561Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler modelYan-Na Guo0Rui-Wei Mou1Meng-Han Lu2Shao-Shan Liang3Yong-Ming He4Lu-Ping Tang5School of Life Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaCorresponding author.; School of Life Science and Engineering, Foshan University, Foshan 528225, ChinaABSTRACT: In order to study the prevention and control EHEC disease measures in poultry, the infection process and development of this disease and the pathological changes of various organs were to be observed. In this study, chickens were infected with different doses of enterohemorrhagic Escherichia coli (EHEC) O157:H7 using different routes of administration to establish EHEC broiler model. A total of 195 14-day-old broilers were randomly divided into 13 groups: including control group, Enema-drip groups (1010, 1011, 1012, 1013 CFUs E. coli O157:H7), gavage groups (P.O) (1011, 1012, 1013, 1014 CFUs E. coli O157:H7), and intraperitoneal injection group (I.P.) (108, 109, 1010, 1011 CFUs E. coli O157:H7). Escherichia coli (E. coli) was given using enema-drip, gavage or intraperitoneal infection. Then the feed intake, weight changes, stool and clinical symptoms of the chicks were recorded during the experiment. 7 d after E. coli infection, blood was collected from the jugular vein and serological tests were carried out. The liver, spleen, and colon of the chicks were extracted to get the organ index, bacteria load, and their histopathological changes. After infection with E. coli, some chicks feces were green or red watery stool, sometimes accompanied by foam, and the material to weight ratio of broilers in I.P. group increased significantly (P < 0.05), the 108 CFUs group were 1.3 times as large as control group. Three modeling methods can result in abnormal serum lipid metabolism and liver function indexes (increase of AST, TBA, T-Bil and TC level; decrease of ALB, TG, and TP level). Infection of chicks with O157:H7 by all 3 methods resulted in its detection in the liver, spleen, and colon. Three modeling methods significantly decreased liver index, and inflammatory cell infiltration and hyperemia were observed in liver. The spleen index in E. coli broilers by gavage and enema-drip was significantly decreased, splenic hyperemia and periarteriolar hyalinosis were observed. The spleen was enlarged with purplish-black spheroids in I.P. group broilers, and the spleen histological changes was more serious. The colon villi of broilers in gavage and enema-drip groups were thinner, more prone to rupture, intestinal lamina propria hyperemia, and inflammatory cell infiltration. Moreover, the number of goblet cells in the mucosal epithelium increased. E. coli O157:H7 can induce liver, spleen and intestinal damage and reduce growth performance of chicks. By comparing these 3 methods, we found that chicks infected with O157:H7 by gavage had more severe liver and intestinal damage, the enema-drip method caused most serious intestinal damage, and I.P. method significantly damaged the liver and spleen of chickens.http://www.sciencedirect.com/science/article/pii/S0032579124001408EHEC O157:H7broilermultiple infection methodsliverspleenintestinal |
| spellingShingle | Yan-Na Guo Rui-Wei Mou Meng-Han Lu Shao-Shan Liang Yong-Ming He Lu-Ping Tang Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler model Poultry Science EHEC O157:H7 broiler multiple infection methods liver spleen intestinal |
| title | Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler model |
| title_full | Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler model |
| title_fullStr | Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler model |
| title_full_unstemmed | Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler model |
| title_short | Three different routes of EHEC O157:H7 infection were used to establish EHEC broiler model |
| title_sort | three different routes of ehec o157 h7 infection were used to establish ehec broiler model |
| topic | EHEC O157:H7 broiler multiple infection methods liver spleen intestinal |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124001408 |
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